Background: Several factors influence the development of the surgical wound healing process. Conventional incisions with a scalpel are always associated with greater haemorrhaging, resulting in increased surgical time and inflammatory reaction, in contrast to CO2-laser incisions. Objective: The study aimed to evaluate the influence of CO2-laser and scalpel skin incisions on the surgical wound healing process Animals: We used a sample of 70 dogs (N = 70) of both genders submitted to elective spay surgeries. Methods: The sample was divided in two groups according to the surgical method performed: group S (scalpel) and group L (CO2 laser). Assessment was made over three time points: T0 (before surgery), T1 (48 hours after surgery) and T2 (8 days after surgery). Blood samples were collected to quantify the variations in white blood cells (WBC), plasmatic protein total (PPT) and serum albumin (ALB). To rate a patient's pain level, we used the Melbourne Pain Scale (MPS). We followed up with the patients to evaluate the wound for closure time, dehiscence and scar appearance. Results: Skin incisions made with the CO2 laser are associated with lower WBC counts and minor tissue trauma because the endothelial wall does not incur as much injury as with scalpel incisions, thus decreasing the PPT and ALB extravasation levels and promoting the healing process. At T1, the individuals in group L had lower pain levels and consequently exhibited greater post-operative patient comfort. The cosmetic results were different between the methods, with 100% of the group L patients showing very good scar appearances eight days after surgery. Conclusion: These findings show that the method selected to perform skin incisions influences the healing process of the affected tissues.