Multiple tandem 18-kb sequences clustered in the region of the acute promyelocytic leukemia breakpoint on chromosome 17

Loss of heterozygosity on chromosomes 17 and 18 in breast carcinoma: two additional regions identified.

“…Moreover, it suggests that it may be possible to determine how the different sets of mutations might collaborate in effecting cancer development. Although an overall test of independence using the Bonferroni adjustment (in which each pairwise P value is multiplied by the number of tests done-i.e., 45) gives no evidence of significant associations, other reports are consistent with this working hypothesis. For instance, the association between LOH on chromosomes lip and 17p in primary breast cancers has also been reported by MacKay et al (35).…”

“…1) suggest that on chromosome 17q, such a gene is located between the centromere and the sequences defined by pRMU3. Within this large region there are several potential candidate targets (43), including the von Recklinghausen neurofibromatosis (NFI) gene (44) and acute promyelocytic leukemia translocation break point (45). The gene(s) affected by LOH on chromosome 18 appear to be located between 18q21.3 and 18q23, near the sequences defined by the anonymous DNA probe OS-4.…”

Flip and Flop: A Cell-Specific Functional Switch in Glutamate-Operated Channels of the CNS

“…The neuronal populations decorated by the cognate flipand flop-specific probes together make up the structures visualized by the respective pan probes (4). Differences in signal intensities relative to those of pan probes reflect the differential stringencies used for these probes (12). This finding is best illustrated for GluR-D mRNA: the flip version is only observed in the cerebellum (Fig.…”

“…Channel conductance and gating kinetics were estimated by fluctuation analysis of whole-cell currents. (12), as well as with an oligonucleotide (5'-CA-CTCTCGATGCCATATACGTTGTAACCTTCC-TTATAAGTTGC-3') complementary to sequences encoding residues 546-859 (ATYKEGYNVYGI-ES) of the mature GluR-B polypeptide (4). Four independent, overlapping clones were isolated, mapped, and sequenced across the specified region.…”

“…Olignucloie n longer. to procedures are,asin(12). CAl Emulsion autoradiographs showing differential disti GluR-A flip (A and C) and flop (B and D) mRNAs at boundary of rat hippocampus.…”