Multiple Substrate Growth Kinetics of <i>Leptothrix discophora</i>SP‐6

Yurt, Nurdan; Sears, Joe; Lewandowski, Zbigniew

doi:10.1021/bp0255098

Cited by 13 publications

(14 citation statements)

References 13 publications

(15 reference statements)

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“…They also presented a K s with a 10% variability (between 0.45 and 0.5 mg·l -1 ) also quite similar to that presented in this work (ranging a 12% between 0.675 and 0.77 mg·l -1 ). The same can be said for the oxygen maintenance coefficient which is also consistent with the values presented for both attached growth systems (Zhou et al 2012) and suspended growth microorganisms (Yurt et al 2002). Thus, the methodology described in this current work represents progress in biofilm research, permitting the study and characterization of mass transport and biokinetics processes through in situ measurements while taking biofilm heterogeneity into consideration.…”

Section: Discussionsupporting

confidence: 83%

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Biofilm dynamics characterization using a novel DO-MEA sensor: mass transport and biokinetics

Guimerà

Moya

Dorado

et al. 2014

Appl Microbiol Biotechnol

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Biodegradation process modeling is an essential tool for the optimization of biotechnologies related to gaseous pollutants treatment. In these technologies, the predominant role of biofilm, particularly under conditions of no mass transfer limitations, results in a need to determine what processes are occurring within the same. By measuring the interior of the biofilms, an increased knowledge of mass transport and biodegradation processes may be attained. This information is useful in order to develop more reliable models that take biofilm heterogeneity into account. In this study, a new methodology, based on a novel dissolved oxygen (DO) and mass transport microelectronic array (MEA) sensor, is presented in order to characterize a biofilm. Utilizing the MEA sensor, designed to obtain DO and diffusivity profiles with a single measurement, it was possible to obtain distributions of oxygen diffusivity and biokinetic parameters along a biofilm grown in a flat plate bioreactor (FPB). The results obtained for oxygen diffusivity, estimated from oxygenation profiles and direct measurements, revealed that changes in its distribution were reduced when increasing the liquid flow rate. It was also possible to observe the effect of biofilm heterogeneity through biokinetic parameters, estimated using the DO profiles. Biokinetic parameters, including maximum specific growth rate, the Monod half-saturation coefficient of oxygen and the maintenance coefficient for oxygen which showed a marked variation across the biofilm, suggest that a tool that considers the heterogeneity of biofilms is essential for the optimization of biotechnologies.2

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Section: Discussionsupporting

confidence: 83%

“…In addition, various studies (Liu and Chen 2009;Yurt et al 2002) have demonstrated the viability of the Monod kinetic equation, widely used in suspended cultures growth models (Brouwer et al 1998), to describe immobilized microbial growth.…”

Section: Biofilm Modelingmentioning

confidence: 99%

Biofilm dynamics characterization using a novel DO-MEA sensor: mass transport and biokinetics

Guimerà

Moya

Dorado

et al. 2014

Appl Microbiol Biotechnol

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“…Minimizing this objective function was required to calculate the biokinetic parameters from the experimental data [16]. To improve the accuracy of the double parametric model, random data sets, similar to the experimental data, within the range of dissolved CO and H 2 tensions and calculated specific growth rates, were generated using standard Excel RAND function.…”

Section: Resultsmentioning

confidence: 99%

Kinetic Studies on Fermentative Production of Biofuel from Synthesis Gas UsingClostridium ljungdahlii

Mohammadi

Mohamed

Najafpour

et al. 2014

The Scientific World Journal

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The intrinsic growth, substrate uptake, and product formation biokinetic parameters were obtained for the anaerobic bacterium, Clostridium ljungdahlii, grown on synthesis gas in various pressurized batch bioreactors. A dual-substrate growth kinetic model using Luong for CO and Monod for H2 was used to describe the growth kinetics of the bacterium on these substrates. The maximum specific growth rate (μ max = 0.195 h−1) and Monod constants for CO (K s,CO = 0.855 atm) and H2 (K s,H2 = 0.412 atm) were obtained. This model also accommodated the CO inhibitory effects on cell growth at high CO partial pressures, where no growth was apparent at high dissolved CO tensions (P CO ∗ > 0.743 atm). The Volterra model, Andrews, and modified Gompertz were, respectively, adopted to describe the cell growth, substrate uptake rate, and product formation. The maximum specific CO uptake rate (q max = 34.364 mmol/gcell/h), CO inhibition constant (K I = 0.601 atm), and maximum rate of ethanol (R max = 0.172 mmol/L/h at P CO = 0.598 atm) and acetate (R max = 0.096 mmol/L/h at P CO = 0.539 atm) production were determined from the applied models.

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“…To prepare the inoculum, a MSVP plate (prepared with Agar Noble (Difco 0142)) was streaked with a frozen stock culture of Leptothrix discophora SP-6 as described by Yurt et al (31). Once the bacteria formed colonies, a single colony was transferred aseptically to 100 mL of MSVP medium in a flask.…”

Section: Methodsmentioning

confidence: 99%

Microbial Fuel Cell using Anaerobic Respiration as an Anodic Reaction and Biomineralized Manganese as a Cathodic Reactant

Rhoads

Beyenal

Lewandowski

2005

Environ. Sci. Technol.

285

135

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We have operated a microbial fuel cell in which glucose was oxidized by Klebsiella pneumoniae in the anodic compartment, and biomineralized manganese oxides, deposited by Leptothrix discophora, were electrochemically reduced in the cathodic compartment. In the anodic compartment, to facilitate the electron transfer from glucose to the graphite electrode, we added a redox mediator, 2-hydroxy-1,4-naphthoquinone. We did not add any redox mediator to the cathodic compartment because the biomineralized manganese oxides were deposited on the surface of a graphite electrode and were reduced directly by electrons from the electrode. We have demonstrated that biomineralized manganese oxides are superiorto oxygen when used as cathodic reactants in microbial fuel cells. The current density delivered by using biomineralized manganese oxides as the cathodic reactant was almost 2 orders of magnitude higher than that delivered using oxygen. Several fuel cells were operated for 500 h, reaching anodic potentials of -441.5 +/- 31 mVscE and cathodic potentials of +384.5 +/- 64 mVscE. When the electrodes were connected by a 50 Ohms resistor, the fuel cell delivered the peak power density of 126.7 +/- 31.5 mW/m2.

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Multiple Substrate Growth Kinetics of Leptothrix discophoraSP‐6

Cited by 13 publications

References 13 publications

Biofilm dynamics characterization using a novel DO-MEA sensor: mass transport and biokinetics

Biofilm dynamics characterization using a novel DO-MEA sensor: mass transport and biokinetics

Kinetic Studies on Fermentative Production of Biofuel from Synthesis Gas UsingClostridium ljungdahlii

Microbial Fuel Cell using Anaerobic Respiration as an Anodic Reaction and Biomineralized Manganese as a Cathodic Reactant

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