Multiple-Stress Tolerance of Ionizing Radiation-Resistant Bacterial Isolates Obtained from Various Habitats: Correlation Between Stresses

Shukla, Manish; Chaturvedi, Ruchi; Tamhane, D. V.; Vyas, Pranav; Archana, G.; Apte, Shree Kumar; Bandekar, Jayant R.; Desai, Anjana J.

doi:10.1007/s00284-006-0311-3

Cited by 59 publications

(52 citation statements)

References 33 publications

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“…Environmental stresses of different types may result in similar damage to bacteria, and survival appears to involve recombination repair and oxidative-stress resistance (Mattimore and Battista, 1996;Billi et al, 2000;Battista et al, 2001;Shukla et al, 2007). In Deinococcus radiodurans, both desiccation and UV irradiation elicit the same dark DNA repair systems (Battista, 1997;Miller et al, 1999;Miller, 2000).…”

Section: Discussionmentioning

confidence: 99%

Bacterial Growth at the High Concentrations of Magnesium Sulfate Found in Martian Soils

et al. 2012

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The martian surface environment exhibits extremes of salinity, temperature, desiccation, and radiation that would make it difficult for terrestrial microbes to survive. Recent evidence suggests that martian soils contain high concentrations of MgSO 4 minerals. Through warming of the soils, meltwater derived from subterranean ice-rich regolith may exist for an extended period of time and thus allow the propagation of terrestrial microbes and create significant bioburden at the near surface of Mars. The current report demonstrates that halotolerant bacteria from the Great Salt Plains (GSP) of Oklahoma are capable of growing at high concentrations of MgSO 4 in the form of 2 M solutions of epsomite. The epsotolerance of isolates in the GSP bacterial collection was determined, with 35% growing at 2 M MgSO 4 . There was a complex physiological response to mixtures of MgSO 4 and NaCl coupled with other environmental stressors. Growth also was measured at 1 M concentrations of other magnesium and sulfate salts. The complex responses may be partially explained by the pattern of chaotropicity observed for high-salt solutions as measured by agar gelation temperature. Select isolates could grow at the high salt concentrations and low temperatures found on Mars. Survival during repetitive freezethaw or drying-rewetting cycles was used as other measures of potential success on the martian surface. Our results indicate that terrestrial microbes might survive under the high-salt, low-temperature, anaerobic conditions on Mars and present significant potential for forward contamination. Stringent planetary protection requirements are needed for future life-detection missions to Mars.

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Section: Discussionmentioning

confidence: 99%

Bacterial Growth at the High Concentrations of Magnesium Sulfate Found in Martian Soils

et al. 2012

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“…Although all known naturally occurring radiation-resistant bacteria are also desiccation resistant, radiation-resistant strains of E. coli evolved under laboratory conditions are not desiccation resistant (238; J. R. Battista, personal communication). Similarly, desiccation tolerance mechanisms do not always result in radiation resistance, as desiccation-tolerant bacteria from the Actinobacteria, Arthrobacter, and Rhodococcus families are not radiation resistant (569).…”

Section: Ionizing Radiation Resistance Of D Radioduransmentioning

confidence: 99%

Oxidative Stress Resistance inDeinococcus radiodurans

Slade

Radman

2011

Microbiol Mol Biol Rev

648

639

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SUMMARY Deinococcus radiodurans is a robust bacterium best known for its capacity to repair massive DNA damage efficiently and accurately. It is extremely resistant to many DNA-damaging agents, including ionizing radiation and UV radiation (100 to 295 nm), desiccation, and mitomycin C, which induce oxidative damage not only to DNA but also to all cellular macromolecules via the production of reactive oxygen species. The extreme resilience of D. radiodurans to oxidative stress is imparted synergistically by an efficient protection of proteins against oxidative stress and an efficient DNA repair mechanism, enhanced by functional redundancies in both systems. D. radiodurans assets for the prevention of and recovery from oxidative stress are extensively reviewed here. Radiation- and desiccation-resistant bacteria such as D. radiodurans have substantially lower protein oxidation levels than do sensitive bacteria but have similar yields of DNA double-strand breaks. These findings challenge the concept of DNA as the primary target of radiation toxicity while advancing protein damage, and the protection of proteins against oxidative damage, as a new paradigm of radiation toxicity and survival. The protection of DNA repair and other proteins against oxidative damage is imparted by enzymatic and nonenzymatic antioxidant defense systems dominated by divalent manganese complexes. Given that oxidative stress caused by the accumulation of reactive oxygen species is associated with aging and cancer, a comprehensive outlook on D. radiodurans strategies of combating oxidative stress may open new avenues for antiaging and anticancer treatments. The study of the antioxidation protection in D. radiodurans is therefore of considerable potential interest for medicine and public health.

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“…Analysis of transcriptome (27,28) and proteome (29,30) changes upon IR exposure, as well as careful analysis of how the genome is reconstructed over time (1,(31)(32)(33)(34), have provided some important insights into this bacterium's response to IR. However, broad genetic screens to identify all contributing processes are very difficult to perform with Deinococcus, reflecting its multigenomic status (1,2,19,24).…”

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confidence: 99%

Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation

Byrne

Chen

Wood

et al. 2014

Journal of Bacteriology

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To further an improved understanding of the mechanisms used by bacterial cells to survive extreme exposure to ionizing radiation (IR), we broadly screened nonessential Escherichia coli genes for those involved in IR resistance by using transposon-directed insertion sequencing (TraDIS). Forty-six genes were identified, most of which become essential upon heavy IR exposure. Most of these were subjected to direct validation. The results reinforced the notion that survival after high doses of ionizing radiation does not depend on a single mechanism or process, but instead is multifaceted. Many identified genes affect either DNA repair or the cellular response to oxidative damage. However, contributions by genes involved in cell wall structure/function, cell division, and intermediary metabolism were also evident. About half of the identified genes have not previously been associated with IR resistance or recovery from IR exposure, including eight genes of unknown function.

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Multiple-Stress Tolerance of Ionizing Radiation-Resistant Bacterial Isolates Obtained from Various Habitats: Correlation Between Stresses

Cited by 59 publications

References 33 publications

Bacterial Growth at the High Concentrations of Magnesium Sulfate Found in Martian Soils

Bacterial Growth at the High Concentrations of Magnesium Sulfate Found in Martian Soils

Oxidative Stress Resistance inDeinococcus radiodurans

Escherichia coli Genes and Pathways Involved in Surviving Extreme Exposure to Ionizing Radiation

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