Multiple steps of leaf thickening during sun‐leaf formation in Arabidopsis

Hoshino, Rina; Yoshida, Yuki; Tsukaya, Hirokazu

doi:10.1111/tpj.14467

Cited by 35 publications

(41 citation statements)

References 63 publications

(127 reference statements)

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“…Similar increases in SLA and reductions in photosynthetic capacity were observed for 1a and all three double mutants. These observations are in line with previous growth analyses of Arabidopsis Rubisco activase antisense lines under high light (Eckardt et al, 1997), where a reallocation of resources to expand leaf area and reduce thickness was observed when photosynthetic capacity was limiting (Hoshino et al, 2019).…”

Section: Discussionsupporting

confidence: 91%

Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis

Khumsupan

Kozlowska

Orr

et al. 2020

Journal of Experimental Botany

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Abstract The primary CO2-fixing enzyme Rubisco limits the productivity of plants. The small subunit of Rubisco (SSU) can influence overall Rubisco levels and catalytic efficiency, and is now receiving increasing attention as a potential engineering target to improve the performance of Rubisco. However, SSUs are encoded for by a family of nuclear rbcS genes in plants, which makes them challenging to engineer and study. Here we have used CRISPR/Cas9 and T-DNA insertion lines to generate a suite of single and multiple gene knockout mutants for the four members of the rbcS family in Arabidopsis, including two novel mutants 2b3b and 1a2b3b. 1a2b3b contained very low levels of Rubisco (ca. 3% relative to WT) and is the first example of a mutant with a homogenous Rubisco pool consisting of a single SSU isoform (1B). Growth under near-outdoor levels of light demonstrated Rubisco-limited growth phenotypes for several SSU mutants and the importance of the 1A and 3B isoforms. We also identified 1a1b as a likely lethal mutation, suggesting a key contributory role for the least expressed 1B isoform during early development. The successful use of CRISPR/Cas here suggests this is a viable approach for exploring the functional roles of SSU isoforms in plants.

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Section: Discussionsupporting

confidence: 91%

Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis

Khumsupan

Kozlowska

Orr

et al. 2020

Journal of Experimental Botany

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“…Plant morphology and physiology exhibits plasticity in response to light (Hoshino, Yoshida, & Tsukaya, 2019; Niinemets, 2007). Growing under FL, leaves may show different acclimation traits compared to leaves grown under constant light (C) (Pao, Stutzel, & Chen, 2019; Vialet‐Chabrand et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

Salt stress and fluctuating light have separate effects on photosynthetic acclimation, but interactively affect biomass

Zhang

Kaiser

Marcelis

et al. 2020

Plant Cell & Environment

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In nature, soil salinity and fluctuating light (FL) often occur concomitantly. However, it is unknown whether salt stress interacts with FL on leaf photosynthesis, architecture, biochemistry, pigmentation, mineral concentrations, as well as whole-plant biomass. To elucidate this, tomato (Solanum lycopersicum) seedlings were grown under constant light (C, 200 μmol m −2 s −1) or FL (5-650 μmol m −2 s −1), in combination with no (0 mM NaCl) or moderate (80 mM NaCl) salinity, for 14 days, at identical photoperiods and daily light integrals. FL and salt stress had separate effects on leaf anatomy, biochemistry and photosynthetic capacity: FL reduced leaf thickness as well as nitrogen, chlorophyll and carotenoid contents per unit leaf area, but rarely affected steady-state and dynamic photosynthetic properties along with abundance of key proteins in the electron transport chain. Salt stress, meanwhile, mainly disorganized chloroplast grana stacking, reduced stomatal density, size and aperture as well as photosynthetic capacity. Plant biomass was affected interactively by light regime and salt stress: FL reduced biomass in salt stressed plants by 17%, but it did not affect biomass of non-stressed plants. Our results stress the importance of considering FL when inferring effects of salt-stress on photosynthesis and productivity under fluctuating light intensities.

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“…However, at a single leaf level, the net photosynthesis under diffuse light was found to be lower than under direct light [5,6]. This is because leaves fully exposed to high irradiance direct light are morphologically adapted by having a thick mesophyll layer, which is not the case for thinner shaded leaves [7]. In these fully exposed thick leaves, diffuse light has a lower penetration depth, which reduces the net photosynthetic capacity compared to direct light.…”

Section: Introductionmentioning

confidence: 96%

The Effect of Low-Haze Diffuse Glass on Greenhouse Tomato and Bell Pepper Production and Light Distribution Properties

Holsteens

Moerkens

Poel

et al. 2020

Plants

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Diffuse greenhouse glass can increase the production and growth of several crops, by scattering the incoming direct sunlight, which results in a better and more homogeneous light distribution in the crop canopy. Tomato and bell pepper growers in Belgium tend to install low-haze diffuse glass with a double anti-reflection (AR) coating. These glass types have a limited diffuse effect but have a higher light transmission compared to standard float glass. Therefore, tomato growers often increase stem density to maximize light interception. However, a denser crop could counteract the positive effects of diffuse glass on the vertical light distribution. In this study, the effect of low-haze diffuse glass with an AR coating was evaluated for different cropping densities for tomato and bell pepper taking into account the vertical light distribution throughout the crop canopy. Tomato plants with two stem densities (3.33 and 3.75 stems.m−2) and bell pepper plants (with only one stem density of 7.1 stems.m−2) were evaluated in a greenhouse compartment with diffuse and reference float glass during a full growing season. For tomato, a significant production increase of 7.5% was observed under diffuse glass during the second half of the growing season but only for the low stem density. The benefit of diffuse glass appears most relevant during sunny clear skies and on the sun-side-facing rows of the crop. For bell pepper, no significant production increases were noted between regular float or diffuse glass, because a bell pepper crop is typically covered with thermal screens to prevent sunburn on the fruits during sunny days. The vertical light distribution and the usefulness of AR-coated diffuse glass depends on the crop type and should be optimized accordingly by altering the stem density, leaf pruning strategy, row orientation, or crop variety.

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Multiple steps of leaf thickening during sun‐leaf formation in Arabidopsis

Cited by 35 publications

References 63 publications

Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis

Generating and characterizing single- and multigene mutants of the Rubisco small subunit family in Arabidopsis

Salt stress and fluctuating light have separate effects on photosynthetic acclimation, but interactively affect biomass

The Effect of Low-Haze Diffuse Glass on Greenhouse Tomato and Bell Pepper Production and Light Distribution Properties

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