Multiple-sites of inhibition by intravenous metyrapone of human adrenal steroidogenesis

Schöneshöfer, M.; Claus, Maximilian

doi:10.1530/acta.0.1090378

Cited by 3 publications

(1 citation statement)

References 19 publications

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“…Under the present conditions of metyrapone infusion, plasma cortisol was found to be suppressed within two hours after Start of infusion (17). Plasma levels increased again from 15.00 h in the low dose experiment and from 16.00 h in the high dose experiment (17).…”

Section: Discussionmentioning

confidence: 51%

20-Dihydroisomers of Cortisol and Cortisone in Human Urine: Excretion Rates under Different Physiological Conditions

Eisenschmid¹,

Heilmann²,

Oelkers³

et al. 1987

Clinical Chemistry and Laboratory Medicine

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The urinary excretion rates of free cortisol and cortisone äs well äs of their 20-dihydroisomers have been studied in normal subjects under different physiological or pharmacological conditions. For the estimation of steroid excretion rates, a fully automated, liquid-chromatographic method was used.In normal subjects, the median steroid excretion rates of free cortisol, cortisone, 20-a-dihydrocortisol, 20-ß-dihydrocortisol, 20-a-dihydrocortisone and 20-ß-dihydrocortisone were 6.7, 8.0, 9.8, 5.2, 5.7 and 1.3 / mpl creatinine.The excretion rates measured at three different intervals of the day followed a circadian rhythm similar to that known for the cortisol secreting activity of the adrenal gland.After adrenal Stimulation by i. v. application of 250 g of tetracosactide hexaacetate, (Synacthen®, corticotro-) excretion of urinary cortisol was sigiiificantly higher than those of the other steroids.During a 24 h infusion of corticotropiii^..^, the excretion rates of cortisol and its C-20 reduced isomers increased to a significantly greater extent than those of cortisone and its C-20 reduced isomers.During a fourJiour infusion of hydrocortisone, the relative increase of cortisol excretion was greater than that of the other steroids.During a five-hour infusion of metyrapone at different dosages, the excretion of all steroids decreased in a ;dose-deperident manner.The present data indicate that the 20^dihydroisomers of cortisol and cortisone in human urine primarily originate from the peripheral metabolism of cortisol rather than from adrenal secretion. A partial secretion of the C-20-reduced isomers of cortisol by the adrenal gland and an Inhibition of 11-oxidoreductase by corticotropin^ >24 can not be excluded. A 24 h Stimulation by corticotropin^.24 is not sufficiently chronic to significantly induce the 20-oxidoreductase. -i J. Clin. Chem. Clin. Biochem. / Vol. 25,1987 / No. 6 JL Brought to you by | University of Manchester Authenticated Download Date | 5/25/15 10:43 PM346 Eisensohmid el al.: 20-Dihydroisomcrs of cortisol and cortisone in human urine

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Section: Discussionmentioning

confidence: 51%