Multiple Shoot Regeneration from Young Shoots of Kenaf (Hibiscus cannabinus)

Light and Scanning Electron Microscopic Analysis of Benzyl Adenine Induced Multiple Shoot Regeneration in Kenaf (Hibiscus cannabinus L.)

“…This result confirms the results of our previous study on multiple shoot regeneration in kenaf (Herath et al 2004). This indicates that in normal kenaf plants apical dominance prevents the axillary shoot development but the apical dominance is eliminated by the treatment Fig.…”

“…Surface sterilization and germination of seed, and the tissue culture conditions, unless otherwise stated, were carried out according to the earlier described method (Herath et al 2004). Shoot tips together with the cotyledonory nodes were isolated from 7-day-old seedlings and cultured on the medium containing MS (Murashige and Skoog 1962) basal salts and vitamins, 3% (w/v) sucrose, BA (2.2, 4.4, 8.8 or 22 µM) and solidified with 0.8% (w/v) agar (INA AGAR BA-30).…”

“…Herbicide-resistant kenaf cultivars would reduce seedling weed competition and make kenaf cultivation more profitable. Several studies were made to develop an in vitro regeneration method, which would facilitate the subsequent development of genetically transformed plants of kenaf (Zapata et al 1999, Srivatanakul et al 2000, Herath et al 2004. Multiple shoots were induced in a young shoot of kenaf by treatment with benzyladenine (BA) (Herath et al 2004).…”

In vitro propagation of Homalomena aromatica Schott., an endangered aromatic medicinal herb of Northeast India

“…In Kn containing medium, the number of shoots were greatly reduced compared to BAP. The inferiority of Kn to BAP has been reported in other plants such as Chlorophytum borivilianum (Purohit et al 1994) and Hibiscus cannabinus (Herath et al 2004). Though TDZ had similar effect as BAP with more or less the same number of shoot production, the explants with the initiated shoots in TDZ containing medium turned brown after shoot initiation and died with no further development.…”

Sterilization of <i>Hibiscus rosa-sinensis</i> L. Vegetative Explants Sourced from Plants Grown in Open Environment and Influences of Organic Ingredients on <i>In Vitro</i> Direct Regeneration

“…Due to the strikingly encouraging effects of plant growth regulator (PGR) in accelerating, inducing, and maintaining in vitro shoots, direct in vitro shoots establishment and multiplication from explants often involve the application of PGRs such as 6-benzylaminopurine (BAP) [12,13], zeatin [14], BAP plus kinetin (KIN) [15], BAP plus indole-3-acetic acid (IAA) [16], or BAP plus naphthalene-3-acetic acid (NAA) [17]. Christensen et al [13]) reported that the most suitable multiplication medium for H. rosa-sinensis L. was demonstrated to be a modified Murashige and Skoog (MS) medium containing 2.2 μM BAP and increased concentrations of calcium at 9 mM and iron at 295 μM provided as ethylenediamine di-2-hydroxyphenyl acetate ferric (Fe-EDDHA).…”