Multiple Shoot Induction from Immature Inflorescence in Sorghum

Abstract

Three improved varieties of Sorghum bicolor (L) Moench SPV 475, CSV 13 and CSV 15 were studied for the regeneration and multiple shoot induction. Explants of young immature inflorescence were cultured on modified Murashige and Skoog medium supplemented with 2% sucrose, 0.8% agar and 1.5 mg/l 2,4-D for callus initiation. Within 9 to 11 d callus initiation was observed. During third week 2 types of calli were identified, one was white compact, nodular embryogenic callus, while the other was unorganized, non morphogenic and non embryogenic callus. After transferring the callus onto regeneration medium (MSϩBAP), somatic embryos were formed, shoot initiation was observed from somatic embryos and was maximum at 1 mg/l BAP (7-17 shoots per culture). For multiple shoot induction different combinations of plant growth regulators (MSϩBAPϩ2,4-D, MSϩGAϩNAA, MSϩBAPϩIAA, MSϩBAPϩGA) along with asparagine and proline (50 mg/l), were used. Among these combinations MSϩ1 mg/l BAPϩ1 mg/l IAA gave maximum shoot induction. In this combination about 11-69 shoots were obtained per culture. And subsequent root formation was obtained with half strength MSϩ1.0 mg/l NAA.

Leaf Discs as a Source Material for Plant Tissue Culture Studies of Sorghum bicolor (L.) Moench.

“…Although, tissue culture from Sorghum was reported previously (Indra and Krishnaveni 2009;Nguyen et al, 2007;Pola et al, 2007;. In many Sorghum tissue culture studies, inflorescence tissue was used as explants for initiating embryogenic cultures (Gupta et al, 2006;Jogeswar et al, 2007;Mani and Pola, 2003). Conversely, Sorghum flowering occurs only once in its life time and for few days only, thereby providing a small window of opportunity to initiate embryogenic cultures.…”