Multiple Selection Criteria for Probiotic Strains with High Potential for Obesity Management

Abstract

Since alterations of the gut microbiota have been shown to play a major role in obesity, probiotics have attracted attention. Our aim was to identify probiotic candidates for the management of obesity using a combination of in vitro and in vivo approaches. We evaluated in vitro the ability of 23 strains to limit lipid accumulation in adipocytes and to enhance the secretion of satiety-promoting gut peptide in enteroendocrine cells. Following the in vitro screening, selected strains were further investigated in vivo, single, or as mixtures, using a murine model of diet-induced obesity. Strain Bifidobacterium longum PI10 administrated alone and the mixture of B. animalis subsp. lactis LA804 and Lactobacillus gasseri LA806 limited body weight gain and reduced obesity-associated metabolic dysfunction and inflammation. These protective effects were associated with changes in the hypothalamic gene expression of leptin and leptin receptor as well as with changes in the composition of gut microbiota and the profile of bile acids. This study provides crucial clues to identify new potential probiotics as effective therapeutic approaches in the management of obesity, while also providing some insights into their mechanisms of action.

Is There a Link between Oropharyngeal Microbiome and Schizophrenia? A Narrative Review

“…A recent study on the management of obesity [ 46 ] using the gut microbiota of mouse models showed that a combination of B. animalis subsp. lactis with Lactobacillus gasseri significantly limited the body weight gain and increase in body fat induced by a high-fat diet (HFD) ( p < 0.05).…”

Lactic Acid Bacteria Strains Differently Modulate Gut Microbiota and Metabolic and Immunological Parameters in High-Fat Diet-Fed Mice

“…There is considerable evidence in pre-clinical and clinical interventions that demonstrates the use of probiotics as an alternative to improve the treatment and prevention of obesityrelated disorders (10,23). The different anti-obesity effects of traditional or not traditional probiotics strains are needed to be well-defined since its benefits are strains and/or dosedependent (24). It is crucial to understand the strain-specificity and mechanisms involved in probiotics.…”

Effects and Mechanisms of Probiotics, Prebiotics, Synbiotics, and Postbiotics on Metabolic Diseases Targeting Gut Microbiota: A Narrative Review

“…The in vitro study showed that the mixture of Bifidobacterium lactis and Lactobacillus salivarius could promote the production of butyrate and propionate in the fermentation system [ 47 ]. Moreover, after comparing 23 bacteria strains, an experimental study revealed that Bifidobacterium longum PI10 and Ligilactobacillus salivarius PI2 could restrict lipid accumulation in adipocytes [ 70 ]. Furthermore, the Lactobacillus plantarum NK3 and Bifidobacterium longum NK49 could mitigate obesity and osteoporosis in mice by suppressing the production of lipopolysaccharide (LPS) from gut microbiota and downregulating nuclear factor kappa B (NF-κB)-linked tumor necrosis factor α (TNF-α) expression [ 71 ].…”

“…Besides, the single administration of Lactobacillus fermentum MCC2759 and MCC2760 was both beneficial to diabetic rats, but the MCC2760 strain showed a slightly better improvement in the intestinal barrier and the expression of GLUT4, GLP1, and zonula occludens-1 (ZO-1) than the MCC2759 strain [ 72 ]. Moreover, Bifidobacterium longum PI10 was a highly potent inducer for GLP1 and interleukin-10 (IL-10), which could strengthen the intestinal barrier [ 70 ].…”