Multiple roles of phosphoinositide-specific phospholipase C isozymes

Suh, Pann‐Ghill; Park, Jae-Il; Manzoli, Lucia; Cocco, Lucio; Peak, Joanna; Katan, Matilda; Fukami, Kiyoko; Kataoka, Tohru; Yun, Sanguk; Ryu, Sung Ho

doi:10.5483/bmbrep.2008.41.6.415

Cited by 439 publications

(485 citation statements)

References 199 publications

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“…21 We detected five PLC isozymes belonging to b (B1, 3), e (E1), and Z (H1, 2) subtypes, of which, PLC-b, and -e are activated by agonist (including vasoactive substances) binding to plasma membrane G protein-coupled receptors. 21 All PLC isozymes detected are known generators of second messengers, inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol. 21 Binding of IP3 to sarcoplasmic reticulum surface receptors (IP3Rs or ITPRs) activates Ca 2 þ release.…”

Section: Cerebrovascular Proteomics a Badhwar Et Almentioning

confidence: 95%

“…21 All PLC isozymes detected are known generators of second messengers, inositol 1,4,5-trisphosphate (IP3) and 1,2-diacylglycerol. 21 Binding of IP3 to sarcoplasmic reticulum surface receptors (IP3Rs or ITPRs) activates Ca 2 þ release. 26 We detected three ITPRs, namely ITPR1, 2, and 3, all previously showed to be present in rat cerebral artery SMCs with ITPR1 being the most abundant.…”

Section: Cerebrovascular Proteomics a Badhwar Et Almentioning

confidence: 99%

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The Proteome of Mouse Cerebral Arteries

Badhwar

Stanimirovic

Hamel

et al. 2014

J Cereb Blood Flow Metab

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The cerebral vasculature ensures proper cerebral function by transporting oxygen, nutrients, and other substances to the brain. Distribution of oxygenated blood throughout the neuroaxis takes place at the level of the circle of Willis (CW). While morphologic and functional alterations in CW arteries and its main branches have been reported in cerebrovascular and neurodegenerative diseases, accompanying changes in protein expression profiles remain largely uncharacterized. In this study, we performed proteomics to compile a novel list of proteins present in mouse CW arteries and its ramifications. Circle of Willis arteries were surgically removed from 6-month-old wild-type mice, proteins extracted and analyzed by two proteomics approaches, gel-free nanoLC-mass spectrometry (MS)/MS and gel-based GelLC-MS/MS, using nanoAcquity UPLC coupled with ESI-LTQ Orbitrap XL. The two approaches helped maximize arterial proteome coverage. Six biologic and two technical replicates were performed. In all, 2,188 proteins with at least 2 unique high-scoring peptides were identified (6,630 proteins total). Proteins were classified according to vasoactivity, blood-brain barrier specificity, tight junction and adhesion molecules, membrane transporters/channels, and extracellular matrix/basal lamina proteins. Furthermore, we compared the identified CW arterial proteome with the published brain microvascular proteome. Our database provides a vital resource for the study of CW cerebral arterial protein expression profiles in health and disease. Keywords: circle of Willis; cerebral artery; cerebral microvessels; proteomics; vascular reactivity INTRODUCTION A healthy cerebral circulation is essential for maintaining brain perfusion and function. 1 In human, oxygenated blood to the brain is delivered by the internal carotid and vertebrobasilar arteries, and is distributed throughout the neuroaxis at the level of the circle of Willis (CW), a ringlike arterial structure located in the subarachnoid space at the base of the brain. The CW is formed by the confluence of the anastomotic branches of the two internal carotid arteries, rostral portion of the vertebrobasilar artery, and the anterior and posterior communicating arteries. 2 The arterial wreath allows for communications between the anterior and posterior circulations providing blood to the forebrain and the hindbrain, respectively, and insures redundancies in the cerebral circulation. 3 The three principal brain arteries arising from the CW are the left and right anterior, middle, and posterior cerebral arteries, cortical branches of which penetrate the brain parenchyma to irrigate the cerebral cortex and deep structures of the brain. 2 In mice, the CW is similarly located along the ventral aspect of the brain extending from the pons-midbrain junction to the anterior cerebrum and involves the same major arteries. 4 Several studies have reported morphologic and protein expression changes in the CW and its surface branches in healthy aging 5 as well as pathologic conditions, such as cereb...

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Section: Cerebrovascular Proteomics a Badhwar Et Almentioning

confidence: 95%

Section: Cerebrovascular Proteomics a Badhwar Et Almentioning

confidence: 99%

The Proteome of Mouse Cerebral Arteries

Badhwar

Stanimirovic

Hamel

et al. 2014

J Cereb Blood Flow Metab

View full text Add to dashboard Cite

show abstract

“…The presence of distinct regulatory domains renders each sub-family susceptible to different mechanisms of activation (Suh et al 2008). Previous studies placed selected PI-PLC enzymes at the convergence point of pathways involved in the Ras GTPase mediated signalling (Hao et al 2009), which also regulates ezrin activation.…”

Section: Introductionmentioning

confidence: 99%

Ezrin-related Phosphoinositide pathway modifies RhoA and Rac1 in human osteosarcoma cell lines

Vasco

Leopizzi²,

Rocca³

2015

J. Cell Commun. Signal.

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Selected Phosphoinositide-specific Phospholipase C (PI-PLC) enzymes occupy the convergence point of the broad range of pathways that promote Rho and Ras GTPase mediated signalling, which also regulate the activation of ezrin, a member of the ezrin-radixin-moesin (ERM) proteins family involved in the metastatic osteosarcoma spread. Previous studies described that in distinct human osteosarcoma cell lines ezrin networks the PI-PLC with complex interplay controlling the expression of the PLC genes, which codify for PI-PLC enzymes. In the present study, we analyzed the expression and the sub-cellular distribution of RhoA and Rac1 respectively after ezrin silencing and after PI-PLC ε silencing, in order to investigate whether ezrin-RhoGTPAses signalling might involve one or more specific PI-PLC isoforms in cultured 143B and Hs888 human osteosarcoma cell lines. In the present experiments, both ezrin and PLCE gene silencing had different effects upon RhoA and Rac1 expression and subcellular localization. Displacements of Ezrin and of RhoA localization were observed, probably playing functional roles.

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“…Phosphoinositide-specific phospholipase C (PLC) enzymes are involved in a number of cellular responses to receptor activation (Rebecchi and Pentyala, 2000;Rhee, 2001;Suh et al, 2008). Among six families of PLC enzymes (β, γ, δ, ε, ζ and η), PLCβ family members (PLCβ1-4) are driven by a wide range of G q/11 -coupled receptors including M 1 /M 3 muscarinic acetylcholine receptors, group I metabotropic glutamate receptors (I-mGluRs), α 1 adrenergic receptors, and the receptors for cholecystokinin, bradykinin, vasopressin, angiotensin II and thromboxane A 2 (Oude Weernink et al, 2007;Rebecchi and Pentyala, 2000;Rhee, 2001;Werry et al, 2003).…”

Section: Introductionmentioning

confidence: 99%