Multiple roles for BMP signaling in cardiac development

Yuasa, Shinsuke; Fukuda, Keiichi

doi:10.1016/j.ddstr.2008.12.001

Cited by 14 publications

(10 citation statements)

References 54 publications

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“…There is evidence that the TGF-β receptor superfamily (Yuasa and Fukuda, 2008) and receptor-related proteins (Conley et al, 2000) exist in vascular cells such as endothelial cells and smooth muscle cells. Thus, vascular cells are also the target cells of BMP-2.…”

Section: Discussionmentioning

confidence: 99%

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

Liu

Zhong²,

Liang³

et al. 2010

J. Zhejiang Univ. Sci. B

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Abstract:In this paper, we investigate the effect and the possible mechanism of high glucose levels on the calcification of human aortic smooth muscle cells (HASMCs). HASMCs were divided into four groups: normal glucose group (NG), osmolality control group (OC), high glucose group (HG, HASMCs culture medium containing 30 mmol/L glucose), and high glucose plus recombinant human Noggin protein (bone morphogenetic protein-2 (BMP-2) antagonist) group (HN). The mRNA levels and the protein expressions of BMP-2 and core binding factor alpha-1 (Cbfα-1) were measured by real-time quantitative polymerase chain reaction (PCR) and Western blot. After induced by 10 mmol/L β-glycerol phosphoric acid, cells were harvested for assessments of alkaline phosphatase (ALP) activities at Days 1, 2, and 3, and intracellular calcium contents at Days 7 and 14, respectively. High glucose levels increased the mRNA levels and the protein expressions of BMP-2 and Cbfα-1 (P<0.05). The expression of Cbfα-1 was partially blocked by Noggin protein (P<0.05), while BMP-2 was not (P>0.05). After being induced by β-glycerol phosphoric acid, high glucose levels increased the ALP activity [(48.63±1.03)

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Section: Discussionmentioning

confidence: 99%

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

Liu

Zhong²,

Liang³

et al. 2010

J. Zhejiang Univ. Sci. B

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“…Genetic manipulation of Drosophila , zebrafish, and mouse embryos has identified a complex milieu of signaling molecules (morphogens) and their cognate receptors that are required for proper cardiovascular development. These include Notch [11,12], sonic hedgehog (Shh) [13,14], and bone morphogenic protein (BMP) signaling (reviewed in [15]) among many others. Increasingly studied, but less well understood, are the effects of mechanical forces on cells as they divide, migrate, and differentiate [16].…”

Section: Mechanical Forces and Mechanotransduction In Cardiovascular mentioning

confidence: 99%

Pulling on my heartstrings

McCormick

Tzima

2016

Current Opinion in Hematology

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Purpose of review Endothelial cells line the surface of the cardiovascular system and display a large degree of heterogeneity due to developmental origin and location. Despite this heterogeneity, all endothelial cells are exposed to wall shear stress (WSS) imparted by the frictional force of flowing blood, which plays an important role in determining the endothelial cell phenotype. Although the effects of WSS have been greatly studied in vascular endothelial cells, less is known about the role of WSS in regulating cardiac function and cardiac endothelial cells. Recent findings Recent advances in genetic and imaging technologies have enabled a more thorough investigation of cardiac hemodynamics. Using developmental models, shear stress sensing by endocardial endothelial cells has been shown to play an integral role in proper cardiac development including morphogenesis and formation of the conduction system. In the adult, less is known about hemodynamics and endocardial endothelial cells, but a clear role for WSS in the development of coronary and valvular disease is increasingly appreciated. Summary Future research will further elucidate a role for WSS in the developing and adult heart, and understanding this dynamic relationship may represent a potential therapeutic target for the treatment of cardiomyopathies.

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“…Among the ∼20 Bmp ligands, Bmp2, 4, 7 and 10 are expressed in the SHF (Liu et al, 2004;Wang et al, 2010;Yuasa and Fukuda, 2008). We examined the expression levels of these Bmp ligands and their receptors in the SHF via RT-qPCR, and the results revealed significantly reduced levels of Bmp7 in Pten deletion mice compared with those of the controls (Fig.…”

Section: Pten-akt Signaling Suppresses Bmp-smad Signaling Through Foxomentioning

confidence: 99%

Akt1 signaling coordinates BMP signaling and β-catenin activity to regulate second heart field progenitor development

et al. 2015

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Second heart field (SHF) progenitors exhibit continued proliferation and delayed differentiation, which are modulated by FGF4/8/10, BMP and canonical Wnt/β-catenin signaling. PTEN-Akt signaling regulates the stem cell/progenitor cell homeostasis in several systems, such as hematopoietic stem cells, intestinal stem cells and neural progenitor cells. To address whether PTEN-Akt signaling is involved in regulating cardiac progenitors, we deleted Pten in SHF progenitors. Deletion of Pten caused SHF expansion and increased the size of the SHF derivatives, the right ventricle and the outflow tract. Cell proliferation of cardiac progenitors was enhanced, whereas cardiac differentiation was unaffected by Pten deletion. Removal of Akt1 rescued the phenotype and early lethality of Pten deletion mice, suggesting that Akt1 was the key downstream target that was negatively regulated by PTEN in cardiac progenitors. Furthermore, we found that inhibition of FOXO by Akt1 suppressed the expression of the gene encoding the BMP ligand (BMP7), leading to dampened BMP signaling in the hearts of Pten deletion mice. Cardiac activation of Akt also increased the Ser552 phosphorylation of β-catenin, thus enhancing its activity. Reducing β-catenin levels could partially rescue heart defects of Pten deletion mice. We conclude that Akt signaling regulates the cell proliferation of SHF progenitors through coordination of BMP signaling and β-catenin activity.

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Multiple roles for BMP signaling in cardiac development

Cited by 14 publications

References 54 publications

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

Effect of high glucose levels on the calcification of vascular smooth muscle cells by inducing osteoblastic differentiation and intracellular calcium deposition via BMP-2/Cbfα-1 pathway

Pulling on my heartstrings

Akt1 signaling coordinates BMP signaling and β-catenin activity to regulate second heart field progenitor development

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