Multiple RNAs expressed from the int-2 gene in mouse embryonal carcinoma cell lines encode a protein with homology to fibroblast growth factors.

Smith, Rosalind; Peters, Gordon; Dickson, Clive

doi:10.1002/j.1460-2075.1988.tb02908.x

Cited by 120 publications

(78 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These include human FLG (hFGFR-1), originally cloned from a human endothelial cell cDNA library (31)(32)(33), its mouse homologue (34), and the corresponding chicken gene cek-J (39,40) and another member of the FGFR family, the mouse bek gene (41). The presence of several FGFRrelated mRNA sequences in the K-562 cells is particularly exciting, as there is also a gene family encoding several different fibroblast growth factors (35)(36)(37)(42)(43)(44)(45)(46).…”

Section: Resultsmentioning

confidence: 99%

Putative tyrosine kinases expressed in K-562 human leukemia cells.

Partanen

Mäkelä

Alitalo

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Tyrosine phosphorylation is important in the transmission of growth and differentiation signals; known tyrosine kinases include several oncoproteins and growth factor receptors. Interestingly, some differentiated cell types, such as erythrocytes and platelets contain high amounts of phosphotyrosine. We analyzed tyrosine kinases expressed in the K-562 chronic myelogenous leukemia cell line, which has a bipotential erythroid and megakaryoblastoid differentiation capacity.

show abstract

Section: Resultsmentioning

confidence: 99%

Putative tyrosine kinases expressed in K-562 human leukemia cells.

Partanen

Mäkelä

Alitalo

et al. 1990

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Signal intensities were estimated by densitometry scanning (Scan Jet 4P, Hewlett-Packard). The fgf-3 probe was the XbaI ± EcoRI fragment from the SP65 int2 plasmid (Smith et al, 1988). To control RNA loading, the membranes were rehybridized following the same technique (except that hybridization was at 378C and washing was twice at room temperature and once at 378C, all in 26SSC, 0.1% SDS) with an actin cDNA probe (insert fragment from Gem-bactin).…”

Section: Ef43fgf-3 Clones and Dot Blot Analysis Of Fgf-3 Expressionmentioning

confidence: 99%

FGF-3 and FGF-4 elicit distinct oncogenic properties in mouse mammary myoepithelial cells

et al. 1998

View full text Add to dashboard Cite

Fibroblast Growth Factors 3 (FGF-3) and 4 (FGF-4) were compared for the e ects they each exert on EF43 mouse cells. This non-transformed mammary cell line appears to be myoepithelial mainly because it expresses a-smooth muscle actin. The EF43 cells were infected with similar vectors that carry either the short fgf-3 sequence (the product of which goes into the secretory pathway), fgf-4 or the selection gene only as control. In syngeneic animals, EF43.fgf-3 cells were tumorigenic only when orthotopically implanted whereas EF43.fgf-4 cells invariably gave rise to aggressive tumors. However, both tumor types were metastatic as evidenced by the blue micrometastases observed when the implanted cells expressed lacZ. In vitro, the FGF-3 producing cells were strongly invasive in matrigel coated chambers whereas the EF43.fgf-4 cells only were invasive in type I-collagen gels. Interestingly, FGF-3 production greatly stimulated the synthesis of pro-MMP-9 (Matrix Metalloprotease-9) and, to a lesser extent, that of pro-MMP-2. FGF-3 also up-regulated the production of plasminogen activators. In contrast, FGF-4 had no e ect on these secretions and the medium conditioned by the EF43.fgf-4 cells displayed the largest plasminogen activator ± inhibitor activity. These results show that FGF-3 and FGF-4 have distinct mechanisms of action on myoepithelial cells.

show abstract

“…F9 embryonal carcinoma cells can differentiate into several early cell lineages in cell culture (15). Treatment with retinoic acid (RA) and dibutyryl cAMP (Bt 2 cAMP) preferentially induces F9 cells to differentiate in parietal endoderm-like cells, which is accompanied by the induction of Fgf-3 (16,17). By using differentiated F9 cells, we previously identified positive and negative regulatory elements within the 5Ј-proximal region of the Fgf-3 promoter, and we concluded that an element designated PS4A was essential for promoter activity (18,19).…”

mentioning

confidence: 99%

SOX7 and GATA-4 Are Competitive Activators of Fgf-3 Transcription

Murakami

Shen

Ishida

et al. 2004

Journal of Biological Chemistry

View full text Add to dashboard Cite

Fgf-3 is expressed in a dynamic and complex spatiotemporal pattern during mouse development. Previous studies identified GATA-4 as a transcription factor that binds the key regulatory element PS4A of the Fgf-3 promoter and stimulates transcription. Here we show that members of the SOX family of transcription factors also bind PS4A and differentially modulate transcription. At least five SOX genes, Sox2, Sox6, Sox7, Sox13, and Sox17, were expressed in F9 cells, and of these, Sox7 and Sox17 were dramatically induced in parallel with Fgf-3 following differentiation into parietal endoderm-like cells with retinoic acid and dibutyryl cAMP. Complexes could be detected on PS4A with SOX2, SOX7, and SOX17 by using nuclear extracts from differentiated F9 cells. However, only Sox7 expression markedly activated the Fgf-3 promoter in these cells. By contrast, SOX2 was a poor activator of Fgf-3 transcription, and when Sox2 was coexpressed with Gata4, it negatively modulated the strong activation mediated by GATA-4. More detailed analyses showed that SOX7 competes with GATA-4 for PS4A occupancy and to activate the Fgf-3 promoter. In situ hybridization analysis showed that Sox7 is co-expressed with Fgf-3 and Gata4 in the parietal endoderm of E7.5 mouse embryos. In culture, GATA-4-deficient embryonal stem cells were shown to express Fgf-3 upon differentiation into embryoid bodies, although at lower levels than were found in wild type embryonal stem cells. This Fgf-3 expression was virtually abolished when Sox7 expression was suppressed by RNA interference. These results show that SOX7 is a potent activator of Fgf-3 transcription.Fibroblast growth factors (Fgfs) 1 compose a large family of related proteins that can induce or confer a range of biological responses on cells such as proliferation, differentiation, survival, and motility (reviewed in Refs.

show abstract

Multiple RNAs expressed from the int-2 gene in mouse embryonal carcinoma cell lines encode a protein with homology to fibroblast growth factors.

Cited by 120 publications

References 37 publications

Putative tyrosine kinases expressed in K-562 human leukemia cells.

Putative tyrosine kinases expressed in K-562 human leukemia cells.

FGF-3 and FGF-4 elicit distinct oncogenic properties in mouse mammary myoepithelial cells

SOX7 and GATA-4 Are Competitive Activators of Fgf-3 Transcription

Contact Info

Product

Resources

About