Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression

Reveal, Brad; García, Carlos Arturo López; Ellington, Andrew D.; Macdonald, Paul M.

doi:10.4161/rna.8.6.17542

Cited by 21 publications

(32 citation statements)

References 51 publications

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“…Inclusion of these Bru-binding sites confers Brudependent translational repression on the GFP-4xBRE mRNA (Reveal et al, 2011). These binding sites also resulted in enrichment of the mRNA in the oocyte (Fig.…”

Section: Resultsmentioning

confidence: 86%

“…These sites are clustered in the AB and C regions, positioned near the 5′ and 3′ ends of the osk 3′ UTR, respectively (Fig. 1A) (Kim-Ha et al, 1995;Reveal et al, 2010Reveal et al, , 2011. GFP reporter mRNAs bearing either AB or C regions (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…These anti-Bru aptamers were selected in vitro for their ability to bind recombinant Bru protein, and they confer translational repression on the GFP reporter mRNA (Reveal et al, 2011). The GFP-anti-Bru aptamer mRNA was enriched in the oocyte (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Multiple cis-acting signals, some weak by necessity, collectively direct robust transport of oskar mRNA to the oocyte

Ryu

Kenny

Gim

et al. 2017

Journal of Cell Science

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Localization of mRNAs can involve multiple steps, each with its own cisacting localization signals and transport factors. How is the transition between different steps orchestrated? We show that the initial step in localization of Drosophila oskar mRNA − transport from nurse cells to the oocyte − relies on multiple cis-acting signals. Some of these are binding sites for the translational control factor Bruno, suggesting that Bruno plays an additional role in mRNA transport. Although transport of oskar mRNA is essential and robust, the localization activity of individual transport signals is weak. Notably, increasing the strength of individual transport signals, or adding a strong transport signal, disrupts the later stages of oskar mRNA localization. We propose that the oskar transport signals are weak by necessity; their weakness facilitates transfer of the oskar mRNA from the oocyte transport machinery to the machinery for posterior localization.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Multiple cis-acting signals, some weak by necessity, collectively direct robust transport of oskar mRNA to the oocyte

Ryu

Kenny

Gim

et al. 2017

Journal of Cell Science

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“…1D). The UAS-GFP-4xBRE transgene has four copies of an isolated 12 bp BRE-type binding site, but no other osk mRNA sequences, and the GFP4xBRE mRNA is translationally repressed in ovaries (Reveal et al 2011). Germline expression of this mRNA with a GAL4 driver resulted in a substantial increase in the rate of egg laying relative to osk ABC BRE − alone.…”

Section: Bru Binding Sites Are Required For Osk Rna Functionmentioning

confidence: 99%

“…Scanning mutations in the osk C region were introduced by PCR, and incorporated into genomic osk transgenes essentially the same as for the Bru binding site mutations. Transgenes UAS-GFP and UAS-GFP-4xBRE (four copies of the BRE sequence TGTTTTATATGT) have been described (Reveal et al 2011). For these transgenes the GFP is mGFP6 (Haseloff 1999).…”

Section: Flies and Transgenesmentioning

confidence: 99%

oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction

Kanke

Jambor

Reich

et al. 2015

RNA

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The Drosophila oskar (osk) mRNA is unusual in that it has both coding and noncoding functions. As an mRNA, osk encodes a protein required for embryonic patterning and germ cell formation. Independent of that function, the absence of osk mRNA disrupts formation of the karyosome and blocks progression through oogenesis. Here we show that loss of osk mRNA also affects the distribution of regulatory proteins, relaxing their association with large RNPs within the germline, and allowing them to accumulate in the somatic follicle cells. This and other noncoding functions of the osk mRNA are mediated by multiple sequence elements with distinct roles. One role, provided by numerous binding sites in two distinct regions of the osk 3 ′ UTR, is to sequester the translational regulator Bruno (Bru), which itself controls translation of osk mRNA. This defines a novel regulatory circuit, with Bru restricting the activity of osk, and osk in turn restricting the activity of Bru. Other functional elements, which do not bind Bru and are positioned close to the 3 ′ end of the RNA, act in the oocyte and are essential. Despite the different roles played by the different types of elements contributing to RNA function, mutation of any leads to accumulation of the germline regulatory factors in the follicle cells.

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Post-transcriptional gene regulation regulates germline stem cell to oocyte transition during Drosophila oogenesis

Blatt

Martin

Breznak

et al. 2020

Current Topics in Developmental Biology

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Multiple RNA binding domains of Bruno confer recognition of diverse binding sites for translational repression

Cited by 21 publications

References 51 publications

Multiple cis-acting signals, some weak by necessity, collectively direct robust transport of oskar mRNA to the oocyte

Multiple cis-acting signals, some weak by necessity, collectively direct robust transport of oskar mRNA to the oocyte

oskar RNA plays multiple noncoding roles to support oogenesis and maintain integrity of the germline/soma distinction

Post-transcriptional gene regulation regulates germline stem cell to oocyte transition during Drosophila oogenesis

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