Multiple Responsive Polymer-Peptide Bioconjugate

Abstract

A novel multiple responsive polymer-peptide bioconjugate, which exhibits responses to calcium ion, pH, and temperature, was presented. In our study, a peptide with calcium ion response was conjugated with a thermoresponsive polymer at the N terminus to generate a polymer-peptide bioconjugate. Several experiments were conducted to characterize the environmental response of the bioconjugate. Results suggest that electrostatic interaction and hydrogen bonding are the driving forces for peptide self-assembly. Additionally, the incorporation of polymer endows new property to the peptide, though it may disrupt the inner-molecular interactions between peptides in some extent. This study will highlight the fundamental understanding of polymer-peptides that are of potential utility as stimuli-responsive biomaterials.

Supramolecular Peptide/Polymer Hybrid Hydrogels for Biomedical Applications

“…using the LLEELLEELEELLEEA (LE) peptide, inspired from the sequence reported by Kuhnle et al . LE forms α‐helix structures in the presence of calcium ions . The rationale behind their conjugate design was to incorporate pH sensitivity through the peptide sequence (endowed by glutamic acid residues, E) and thermo‐responsiveness through NIPAAm chains.…”

“…Reversible hydrogel formation was shown upon exposure to HCl (gel) and ammonium hydroxide (solution) vapors. The second responsive stimulus, the LCST of the NIPAAm, was studied over different pHs and a slight change from 33 to 38 °C for pH 7 and pH 9 was observed, respectively …”

“…[40,72] Alternative peptide-PNIPAAm conjugates were also described by Zhou et al using the LLEELLEELEELLEEA (LE) peptide, inspired from the sequence reported by Kuhnle et al [70] LE forms α-helix structures in the presence of calcium ions. [73] The rationale behind their conjugate design was to incorporate pH sensitivity through the peptide sequence (endowed by glutamic acid residues, E) and thermo-responsiveness through NIPAAm chains. In ATRP reaction, the common NIPAAm polymerization technique, the modified peptide acted as initiator.…”

“…www.mbs-journal.de over different pHs and a slight change from 33 to 38 °C for pH 7 and pH 9 was observed, respectively. [73] Peptide-polymer conjugates were also explored to form hydrogels through a double-network (DN) approach aimed at mimicking the mechanical properties of natural articular cartilage. Polyacrylamide (PAM) was used to provide a chemically crosslinked polymer network whereas a short ionic complementary octapeptide (KFEFKFEF, named as EFK) enabled the formation of supramolecular network (self-assembled nanofibers).…”

Novel Peptide–Polymer Conjugate with pH-Responsive Targeting/Disrupting Effects on Biomembranes

“…Polymers , have been widely used in nanoscale agents for their adjustable molecular weight distribution and alterable functionality under relevant physiological conditions. Modifying polymers with peptides will be a strategy to improve the functionality of AMPs. , Researchers have proved that some peptides modified with PEG protect them from proteolysis. , Kumar et al developed the novel conjugates AMP–HPG to improve their antimicrobial activity. The Tamerler group conjugated methacrylate to AMP, and the resulting MA–AMP demonstrated significant antimicrobial activity against S.…”