Multiple regulators and their interactions in vivo and in vitro with the cbb regulons of Rhodobacter capsulatus11Edited by N.-H. Chua

Vichivanives, Padungsri; Bird, Terry H.; Bauer, Carl E.; Tabita, F. Robert

doi:10.1006/jmbi.2000.3914

Cited by 58 publications

(91 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The gel shift assay showed that CbbR1 bound to the overlapping promoter region between cbbLS-1 and cbbR1, suggesting that the expression of CbbR1 is subjected to autoregulation. Negative autoregulation has also been demonstrated for the LTTR including CbbR (Schell, 1993;Vichivanives et al, 2000;van Keulen et al, 2003). The amount of the cbbRm transcripts did not vary significantly under any of the CO 2 conditions.…”

Section: Expression Analyses Of Cbbr1 and Cbbrmmentioning

confidence: 72%

“…CbbR, one of the LysR-type transcriptional regulators (LTTRs), has been found in many photo-and chemoautotrophic bacteria, and has been shown to be required to activate expression of RubisCO (Windhövel & Bowien, 1991;Falcone & Tabita, 1993;Gibson & Tabita, 1993;van den Bergh et al, 1993;Paoli et al, 1998;Dubbs et al, 2000Dubbs et al, , 2004Vichivanives et al, 2000). Although the cbbR genes are located upstream of genes encoding RubisCO in a divergent orientation in most bacteria, the number of cbbR genes is not equivalent to that of genes for RubisCO (Windhövel & Bowien, 1991;Gibson & Tabita, 1993;Meijer et al, 1996;Kusian & Bowien, 1997;Smith & Tabita, 2002;Dubbs & Tabita, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…These results indicated that H. marinus has a hierarchical interactive regulatory mechanism among the three RubisCOs. R. capsulatus possesses two cbb operons, and the expression of each operon is activated by the cognate CbbR regulators (Vichivanives et al, 2000). An interactive regulation of the two cbb operons by the cross-reactions of the CbbR regulators is indicated in R. capsulatus.…”

mentioning

confidence: 99%

See 2 more Smart Citations

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

et al. 2005

View full text Add to dashboard Cite

Hydrogenovibrio marinus MH-110 possesses three different sets of genes for ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO): two form I (cbbLS-1 and cbbLS-2) and one form II (cbbM). We have previously shown that the expression of these RubisCO genes is dependent on the ambient CO 2 concentration. LysR-type transcriptional regulators, designated CbbR1 and CbbRm, are encoded upstream of the cbbLS-1 and cbbM genes, respectively. In this study, we revealed by gel shift assay that CbbR1 and CbbRm bind with higher affinity to the promoter regions of cbbLS-1 and cbbM, respectively, and with lower affinity to the other RubisCO gene promoters. The expression patterns of the three RubisCOs in the cbbR1 and the cbbRm gene mutants showed that CbbR1 and CbbRm were required to activate the expression of cbbLS-1 and cbbM, respectively, and that neither CbbR1 nor CbbRm was required for the expression of cbbLS-2. The expression of cbbLS-1 was significantly enhanced under high-CO 2 conditions in the cbbRm mutant, in which the expression of cbbM was decreased. Although cbbLS-2 was not expressed under high-CO 2 conditions in the wild-type strain or the single cbbR mutants, the expression of cbbLS-2 was observed in the cbbR1 cbbRm double mutant, in which the expression of both cbbLS-1 and cbbM was decreased. These results indicate that there is an interactive regulation among the three RubisCO genes.

show abstract

Section: Expression Analyses Of Cbbr1 and Cbbrmmentioning

confidence: 72%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

et al. 2005

View full text Add to dashboard Cite

show abstract

“…Maximal cbb expression during phototrophic growth has been shown to be dependent on cbbR, as well as reg (12,13,18). Thus far, the involvement of upstream activating sequences appears to be unique to R. sphaeroides as such sequences are not involved with the regulation of the cbb I and cbb II operons of the related organism R. capsulatus (19).…”

Section: Discussionmentioning

confidence: 99%

“…It was subsequently shown that regA is required for cbb I and cbb II expression during incubation under photoautotrophic growth conditions (18). It has also been demonstrated that RegA binds directly to cbb operon promoters in both R. capsulatus and R. sphaeroides (18,19). A growing number of studies have shown that the regA-regB (prrA-prrB) two-component system and its homologs regulate the expression of genes involved in a wide variety of metabolic processes such as nitrogen fixation and nitrogen metabolism (20 -22), hydrogen utilization and evolution (20,22), electron transport (23), and the oxidation of formaldehyde (24).…”

mentioning

confidence: 99%

Interactions of the cbbII Promoter-Operator Region with CbbR and RegA (PrrA) Regulators Indicate Distinct Mechanisms to Control Expression of the Two cbb Operons of Rhodobacter sphaeroides

Dubbs

Tabita

2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

In a previous study (Dubbs, J. M., Bird, T. H., Bauer, C. E., and Tabita, F. R. (2000) J. Biol. Chem. 275, 19224 -19230), it was demonstrated that the regulators CbbR and RegA (PrrA) interacted with both promoter proximal and promoter distal regions of the form I (cbb I ) promoter operon specifying genes of the Calvin-BensonBassham cycle of Rhodobacter sphaeroides. To determine how these regulators interact with the form II (cbb II ) promoter, three cbbF II ::lacZ translational fusion plasmids were constructed containing various lengths of sequence 5 to the cbb II operon of R. sphaeroides CAC. Expression of ␤-galactosidase was monitored under a variety of growth conditions in both the parental strain and knock-out strains that contain mutations that affect synthesis of CbbR and RegA. The binding sites for both CbbR and RegA were determined by DNase I footprinting. A region of the cbb II promoter from ؉38 to ؊227 bp contained a CbbR binding site and conferred low level regulated cbb II expression. The region from ؊227 to ؊1025 bp contained six RegA binding sites and conferred enhanced cbb II expression under all growth conditions. Unlike the cbb I operon, the region between ؊227 and ؊545 bp that contains one RegA binding site, was responsible for the majority of the observed enhancement. Both RegA and CbbR were required for maximal cbb II expression. Two potentially novel and specific cbb II promoter-binding proteins that did not interact with the cbb I promoter region were detected in crude extracts of R. sphaeroides. These results, combined with the observation that chemoautotrophic expression of the cbb I operon is RegA independent, indicated that the mechanisms controlling cbb I and cbb II operon expression during chemoautotrophic growth are quite different.The nonsulphur purple bacterium Rhodobacter sphaeroides utilizes the Calvin-Benson-Bassham (CBB) 1 reductive pentose cycle as its primary pathway for CO 2 fixation. In this metabolically diverse organism the CBB cycle plays two very different roles. Under autotrophic growth conditions, CO 2 serves as the sole carbon source, and the CBB cycle is the primary source for nearly all of the fixed carbon utilized by the cell. This may entail aerobic chemoautotrophic growth in the dark (i.e. in a minimal medium lacking organic carbon under an atmosphere of 5% CO 2 /45% H 2 /50% air) or anaerobic photoautotrophic growth in the light (i.e. in a minimal medium bubbled with 1.5% CO 2 /98.5% H 2 ). Photoheterotrophic growth in the presence of a fixed carbon source causes the role of the CBB cycle to shift, such that CO 2 serves primarily as an electron sink, with excess reducing equivalents generated by the oxidation of fixed carbon compounds funneled to CO 2 (1). When grown under conditions where the CBB cycle is required, R. sphaeroides maintains the appropriate level of CBB cycle activity through the coordinate expression of two CBB cycle operons, denoted cbb I and cbb II (2, 3). In addition to structural genes that encode CBB cycle enzymes, each operon encodes one ...

show abstract

The RegB/RegA two-component regulatory system controls synthesis of photosynthesis and respiratory electron transfer components in Rhodobacter capsulatus

Swem

Elsen

Bird

et al. 2001

Journal of Molecular Biology

103

134

View full text Add to dashboard Cite

Multiple regulators and their interactions in vivo and in vitro with the cbb regulons of Rhodobacter capsulatus11Edited by N.-H. Chua

Cited by 58 publications

References 53 publications

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

The role of two CbbRs in the transcriptional regulation of three ribulose-1,5-bisphosphate carboxylase/oxygenase genes in Hydrogenovibrio marinus strain MH-110

Interactions of the cbbII Promoter-Operator Region with CbbR and RegA (PrrA) Regulators Indicate Distinct Mechanisms to Control Expression of the Two cbb Operons of Rhodobacter sphaeroides

The RegB/RegA two-component regulatory system controls synthesis of photosynthesis and respiratory electron transfer components in Rhodobacter capsulatus

Contact Info

Product

Resources

About