Multiple Receptors Contribute to the Attractive Response of Caenorhabditis elegans to Pathogenic Bacteria

Cheng, Wanli; Xue, Hua; Yang, Xue; Huang, Dian; Cai, Minmin; Huang, Feng; Zheng, Longyu; Peng, Donghai; Thomashow, Linda S.; Weller, David M.; Zhou, Yu; Zhang, Jibin

doi:10.1128/spectrum.02319-22

Cited by 2 publications

(1 citation statement)

References 49 publications

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“…Additionally, G proteins activate its Gα (ODR3) subunit, downstream to which transient receptor potential vanilloid or TRPV (OSM9/OCR2) channel is opened and transmembrane Ca 2+ influx occurs [ 28 ]. Opening of CNG and TRPV channels causes behavioral changes in nematode chemotaxis [ 15 , 29 ]. Only a few C. elegans chemosensory gene complements have been identified and functionally validated in PPNs including M. incognita and Heterodera glycines [ 16 , 30 , 31 ].…”

Section: Discussionmentioning

confidence: 99%

Induced knockdown of Mg-odr-1 and Mg-odr-3 perturbed the host seeking behavior of Meloidogyne graminicola in rice

Dutta,

Akhil,

Kundu

et al. 2024

Heliyon

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Section: Discussionmentioning

confidence: 99%

Induced knockdown of Mg-odr-1 and Mg-odr-3 perturbed the host seeking behavior of Meloidogyne graminicola in rice

Dutta,

Akhil,

Kundu

et al. 2024

Heliyon

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Molecular and functional characterization of chemosensory genes from the root-knot nematode Meloidogyne graminicola

Dutta,

Akhil,

Dash

et al. 2023

BMC Genomics

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Background Root-knot nematode Meloidogyne graminicola has emerged as a major threat in rice agroecosystems owing to climate change-induced changes in cultivation practices. Synthetic nematicides are continually being withdrawn from the nematode management toolbox because of their ill effects on the environment. A sustainable strategy would be to develop novel nematicides or resistant plants that would target nematode sensory perception, which is a key step in the host finding biology of plant-parasitic nematodes (PPNs). However, compared to the extensive literature on the free-living nematode Caenorhabditis elegans, negligible research has been performed on PPN chemosensory biology. Results The present study characterizes the five chemosensory genes (Mg-odr-7, Mg-tax-4, Mg-tax-4.1, Mg-osm-9, and Mg-ocr-2) from M. graminicola that are putatively associated with nematode host-finding biology. All the genes were highly transcribed in the early life stages, and RNA interference (RNAi)-induced downregulation of each candidate gene perturbed the normal behavioural phenotypes of M. graminicola, as determined by examining the tracking pattern of juveniles on Pluronic gel medium, attraction to and penetration in rice root tip, and developmental progression in rice root. In addition, a detrimental effect on nematode chemotaxis towards different volatile and nonvolatile organic compounds and host root exudates was documented. Conclusion Our findings enrich the existing literature on PPN chemosensory biology and can supplement future research aimed at identifying a comprehensive chemosensory signal transduction pathway in PPNs.

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Multiple Receptors Contribute to the Attractive Response of Caenorhabditis elegans to Pathogenic Bacteria

Abstract: Nematodes feed on nontoxic bacteria as a food resource and avoid toxic bacteria; they distinguish them through their volatile metabolites. However, the mechanism of how nematodes recognize bacteria by volatile metabolites is not fully understood.

Cited by 2 publications

References 49 publications

Induced knockdown of Mg-odr-1 and Mg-odr-3 perturbed the host seeking behavior of Meloidogyne graminicola in rice

Induced knockdown of Mg-odr-1 and Mg-odr-3 perturbed the host seeking behavior of Meloidogyne graminicola in rice

Molecular and functional characterization of chemosensory genes from the root-knot nematode Meloidogyne graminicola

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