Multiple-Pathogen Inoculation of Cucumber (Cucumis sativus) Seedlings

Wyszogrodzka, Anna J.; Williams, Paul H.; Peterson, C. E.

doi:10.1094/pd-71-0275

Cited by 18 publications

(12 citation statements)

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“…priority effects, in which, for example, inoculation by a virulent pathogen facilitates infection in a subsequent inoculation by a normally avirulent pathogen (41)(42)(43). However, when a virulent and an avirulent pathogen are simultaneously inoculated, such priority effects are generally absent (44)(45)(46). Because in our study different genotypes in an inoculum were introduced to a plant simultaneously, it seems unlikely that there would have been sufficient time for defenses to be activated to prevent at least some infection by the virulent genotypes.…”

Section: Discussionmentioning

confidence: 99%

Evolution of host range inColeosporium ipomoeae, a plant pathogen with multiple hosts

Chappell

Rausher

2016

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

Evolution of host range inColeosporium ipomoeae, a plant pathogen with multiple hosts

Chappell

Rausher

2016

Proc. Natl. Acad. Sci. U.S.A.

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“…Several studies have also investigated the genetic basis of these resistances. For example, the widely used cucumber germplasm lines Gy14 and WI 2757 are resistant to DM, ALS and AR with the resistances derived from PI 197087 (Peterson et al, 1982;Wyszogrodzka et al, 1987). Barnes & Epps (1952, 1954 found the nearly immune resistance to AR of PI 197087 in field tests.…”

Section: Introductionmentioning

confidence: 99%

STAYGREEN, STAY HEALTHY: a loss‐of‐susceptibility mutation in the STAYGREEN gene provides durable, broad‐spectrum disease resistances for over 50 years of US cucumber production

Wang

Tan

et al. 2018

New Phytologist

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The Gy14 cucumber (Cucumis sativus) is resistant to oomyceteous downy mildew (DM), bacterial angular leaf spot (ALS) and fungal anthracnose (AR) pathogens, but the underlying molecular mechanisms are unknown. Quantitative trait locus (QTL) mapping for the disease resistances in Gy14 and further map-based cloning identified a candidate gene for the resistant loci, which was validated and functionally characterized by spatial-temporal gene expression profiling, allelic diversity and phylogenetic analysis, as well as local association studies. We showed that the triple-disease resistances in Gy14 were controlled by the cucumber STAYGREEN (CsSGR) gene. A single nucleotide polymorphism (SNP) in the coding region resulted in a nonsynonymous amino acid substitution in the CsSGR protein, and thus disease resistance. Genes in the chlorophyll degradation pathway showed differential expression between resistant and susceptible lines in response to pathogen inoculation. The causal SNP was significantly associated with disease resistances in natural and breeding populations. The resistance allele has undergone selection in cucumber breeding. The durable, broad-spectrum disease resistance is caused by a loss-of-susceptibility mutation of CsSGR. Probably, this is achieved through the inhibition of reactive oxygen species over-accumulation and phytotoxic catabolite over-buildup in the chlorophyll degradation pathway. The CsSGR-mediated host resistance represents a novel function of this highly conserved gene in plants.

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“…However, susceptibility to multiple pathogens hinders their production increase and quality improvement [1-5] The NBS-LRR resistance (R) genes, which encode proteins containing nucleotide binding sites (NBS) and leucine-rich repeat (LRR) domains, form the largest R-gene family among plant genomes [6]. Therefore, a systematic evaluation of NBS-encoding genes is required in order to better understand cucumber resistance and susceptibility.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of NBS-encoding disease resistance genes in Cucumis sativusand phylogenetic study of NBS-encoding genes in Cucurbitaceae crops

Wan

Wei²,

Bo³

et al. 2013

BMC Genomics

102

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BackgroundPlant nucleotide-binding site (NBS)-leucine-rich repeat (LRR) proteins encoded by resistance genes play an important role in the responses of plants to various pathogens, including viruses, bacteria, fungi, and nematodes. In this study, a comprehensive analysis of NBS-encoding genes within the whole cucumber genome was performed, and the phylogenetic relationships of NBS-encoding resistance gene homologues (RGHs) belonging to six species in five genera of Cucurbitaceae crops were compared.ResultsCucumber has relatively few NBS-encoding genes. Nevertheless, cucumber maintains genes belonging to both Toll/interleukine-1 receptor (TIR) and CC (coiled-coil) families. Eight commonly conserved motifs have been established in these two families which support the grouping into TIR and CC families. Moreover, three additional conserved motifs, namely, CNBS-1, CNBS-2 and TNBS-1, have been identified in sequences from CC and TIR families. Analyses of exon/intron configurations revealed that some intron loss or gain events occurred during the structural evolution between the two families. Phylogenetic analyses revealed that gene duplication, sequence divergence, and gene loss were proposed as the major modes of evolution of NBS-encoding genes in Cucurbitaceae species. Compared with NBS-encoding sequences from the Arabidopsis thaliana genome, the remaining seven TIR familes of NBS proteins and RGHs from Cucurbitaceae species have been shown to be phylogenetically distinct from the TIR family of NBS-encoding genes in Arabidopsis, except for two subfamilies (TIR4 and TIR9). On the other hand, in the CC-NBS family, they grouped closely with the CC family of NBS-encoding genes in Arabidopsis. Thus, the NBS-encoding genes in Cucurbitaceae crops are shown to be ancient, and NBS-encoding gene expansions (especially the TIR family) may have occurred before the divergence of Cucurbitaceae and Arabidopsis.ConclusionThe results of this paper will provide a genomic framework for the further isolation of candidate disease resistance NBS-encoding genes in cucumber, and contribute to the understanding of the evolutionary mode of NBS-encoding genes in Cucurbitaceae crops.

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Multiple-Pathogen Inoculation of Cucumber (Cucumis sativus) Seedlings

Cited by 18 publications

References 0 publications

Evolution of host range inColeosporium ipomoeae, a plant pathogen with multiple hosts

Evolution of host range inColeosporium ipomoeae, a plant pathogen with multiple hosts

STAYGREEN, STAY HEALTHY: a loss‐of‐susceptibility mutation in the STAYGREEN gene provides durable, broad‐spectrum disease resistances for over 50 years of US cucumber production

Genome-wide analysis of NBS-encoding disease resistance genes in Cucumis sativusand phylogenetic study of NBS-encoding genes in Cucurbitaceae crops

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