Multiple origins of a single point mutation in the cotton bollworm tetraspanin gene confers dominant resistance to Bt cotton

Guan, Fang Xia; Hou, Bofeng; Dai, Xiaofeng; Liu, Sitong; Liu, Juanjuan; Gu, Yan; Jin, Lin; Yang, Yihua; Fabrick, Jeffrey A.; Wu, Yi

doi:10.1002/ps.6192

Cited by 15 publications

(21 citation statements)

References 51 publications

(114 reference statements)

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“…We produced SCD-KI by using CRISPR/Cas9 to knock into SCD a single-base pair substitution (T92C) in a gene (HaTSPAN1) encoding a tetraspanin protein (Jin et al, 2018). The T92C mutation causes non-recessive resistance to Cry1Ac in SCD-KI and occurs naturally in field populations of this pest in China (Jin et al, 2018;Guan et al, 2021). We created C2/C3-KO by using CRISPR/Cas9 to knock out two genes (HaABCC2 and HaABCC3) in SCD encoding the ABC transporter proteins ABCC2 and ABCC3 .…”

Section: Introductionmentioning

confidence: 99%

Evaluating Cross-Resistance to Cry and Vip Toxins in Four Strains of Helicoverpa armigera With Different Genetic Mechanisms of Resistance to Bt Toxin Cry1Ac

Hanyang

Zeng

et al. 2021

Front. Microbiol.

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Evolution of resistance by pests has diminished the efficacy of transgenic crops producing insecticidal proteins from Bacillus thuringiensis (Bt). In China, where transgenic cotton producing Bt toxin Cry1Ac has been planted since 1997, field control failures have not been reported but the frequency of resistance to Cry1Ac has increased in the cotton bollworm, Helicoverpa armigera. This provides incentive to switch to multi-toxin Bt cotton, which is grown in many other countries. Previous work created four laboratory strains of H. armigera with >100-fold resistance to Cry1Ac, with the genetic basis of resistance known in all but the LF256 strain. Here, we analyzed the genetic basis of resistance in Cry1Ac in LF256 and evaluated cross-resistance of all four strains to three toxins produced by widely planted multi-toxin Bt cotton: Cry1Fa, Cry2Ab, and Vip3Aa. DNA sequencing revealed that LF256 lacked the mutations in three genes (HaTSPAN1, HaABCC2, and HaABCC3) that confer resistance to Cry1Ac in two other strains of H. armigera we analyzed. Together with previous results, the data reported here show that each of the four strains examined has a different genetic basis of resistance to Cry1Ac. Significant positive cross-resistance occurred to Cry1Fa in three of the four strains tested but not to Cry2Ab or Vip3Aa in any strain. Thus, Cry2Ab and Vip3Aa are likely to be especially valuable for increasing the efficacy and durability of Bt cotton against H. armigera populations that have some resistance to Cry1Ac.

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Section: Introductionmentioning

confidence: 99%

Evaluating Cross-Resistance to Cry and Vip Toxins in Four Strains of Helicoverpa armigera With Different Genetic Mechanisms of Resistance to Bt Toxin Cry1Ac

Hanyang

Zeng

et al. 2021

Front. Microbiol.

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“…Currently, based on its high reliability and low costs, amplicon sequencing has been applied in a series of entomological research, 32–40,46–53 particularly in the detection of the mutation frequency of genes associated with pesticide resistance 30,31 . We detected the frequencies of the L925I and T929V mutations using amplicon sequencing in this study, and found that there was no significant difference in the frequencies of the two mutations in the SX population obtained by Sanger sequencing and amplicon sequencing.…”

Section: Discussionmentioning

confidence: 81%

“…Recently, the frequencies of the T92C mutation in tetraspanin HaTSPAN1 linked with Cry1Ac‐resistance in a geographic population of Helicoverpa armigera in 4 years were detected by the two methods and no significant difference was discovered between the two sequencing data sets 30 . Subsequently, to study the multiple origins of T92C mutation in H. armigera , frequencies of the mutation in 28 field‐collected populations from China were detected by amplicon sequencing and Sanger sequencing, and pair‐wise comparisons between the two data sets showed no significant difference 31 . These findings and our study revealed that amplicon sequencing could be a reliable approach to detect the resistant allele frequencies in insects.…”

Section: Discussionmentioning

confidence: 99%

“…The whole‐genome sequencing technique was conducted to detect mutations involved in resistance to pyrethroids in Halotydeus destructor and Bt protein in Spodoptera frugiperda 28,29 . Recently, amplicon sequencing had been used to detect the frequency of T92C mutation in a tetraspanin associated with resistance to the Cry1Ac protein in populations of Helicoverpa armigera 30,31 . Before being applied in mutation detection, amplicon sequencing had been widely used in in insect–microbial interaction.…”

Section: Introductionmentioning

confidence: 99%

“…28,29 Recently, amplicon sequencing had been used to detect the frequency of T92C mutation in a tetraspanin associated with resistance to the Cry1Ac protein in populations of Helicoverpa armigera. 30,31 Before being applied in mutation detection, amplicon sequencing had been widely used in in insect-microbial interaction. Microbial communities in insects and their host plants were determined by using amplicon sequencing to study intestinal physiology, life stage, diet, microbial transmission and microbe-mediated resistance.…”

Section: Introductionmentioning

confidence: 99%

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Amplicon sequencing detects mutations associated with pyrethroid resistance in Bemisia tabaci (Hemiptera: Aleyrodidae)

Wei¹,

Guan

Wang³

et al. 2021

Pest Management Science

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BACKGROUND Bemisia tabaci (Gennadius) is a major damaging agricultural pest that exhibits high resistance to pyrethroid insecticides. L925I (TTA to ATA) and T929V (ACT to GTT) mutations in the para‐type voltage‐gated sodium channel (VGSC) are associated with resistance of B. tabaci to pyrethroids. Amplicon sequencing is a reliable and highly efficient method to detect the frequency of mutations linked with insecticide resistance. RESULTS Similar frequencies of L925I and T929V mutations were obtained by amplicon sequencing and Sanger sequencing (L925I: 0.3548 vs 0.3619; T929V: 0.6140 vs 0.6381) with overlap of 95% confidence interval in the SX population of B. tabaci. In five populations of B. tabaci from China, the maximum and minimum frequencies of the two mutations were found in the LN (L925I: 0.1126; T929V: 0.8834) and JS (L925I: 0.8776; T929V: 0.1166) populations by amplicon sequencing. However, there was no significant difference in frequencies between the L925I and T929V mutations. The sum frequency of L925I and T929V exceeded 0.9688 in all populations. In addition, a combining mutation, L925 + T929V (L925I and T929V located in same allele), was found in five populations by amplicon sequencing even though its highest frequency was only 0.0157. CONCLUSION We established an efficient approach for detecting frequency of mutation by amplicon sequencing. The frequencies of L925I and T929V in VGSC associated with pyrethroid resistance were detected in this study, which could provide foundational data for resistance management of B. tabaci. © 2021 Society of Chemical Industry.

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A feasibility trial of genomics‐based diagnosis detecting insecticide resistance of the diamondback moth

Yamanaka

Kitabayashi

Jouraku

et al. 2022

Pest Management Science

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BACKGROUND Insecticide resistance management has been key for crop protection for over 70 years and is increasingly important because the development of new active ingredients has decreased in recent years. By monitoring the development of resistance in a timely manner, we can effectively prolong insecticide efficacy. Genomic‐based diagnosis can reliably predict resistance development if information on resistant mutations against major pesticides is available. Here, we developed a feasibility trial of genomics‐based diagnosis of insecticide resistance in diamondback moth (Plutella xylostella) populations in Nagano Prefecture, Japan. Amplicon sequencing analyses using a next‐generation sequencer (Illumina MiSeq) for major insecticides, including diamides, pyrethroids, Bacillus thuringiensis (Bt) toxin (Cry1Ac), organophosphates, and spinosyns, were conducted. RESULTS Mutations related to the resistance of pyrethroids, organophosphates, and diamides (flubendiamide and chlorantraniliprole) prevailed, while those of a diamide (cyantraniliprole), Bt (Cry1Ac), and spinosyns were scanty, suggesting that they are still effective. The results of the genomics‐based diagnosis were generally concordant with the results of bioassays. Resistance development tendencies were generally uniform across Nagano. CONCLUSION An insecticide‐resistance management campaign can be conducted in Nagano Prefecture with a quick genomic‐based diagnosis in early spring while bioassay is the only option for monitoring resistances whose mutations are unavailable. Our study is the first step in the future management of insecticide resistance in all significant pests. © 2022 Society of Chemical Industry.

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Multiple origins of a single point mutation in the cotton bollworm tetraspanin gene confers dominant resistance to Bt cotton

Cited by 15 publications

References 51 publications

Evaluating Cross-Resistance to Cry and Vip Toxins in Four Strains of Helicoverpa armigera With Different Genetic Mechanisms of Resistance to Bt Toxin Cry1Ac

Evaluating Cross-Resistance to Cry and Vip Toxins in Four Strains of Helicoverpa armigera With Different Genetic Mechanisms of Resistance to Bt Toxin Cry1Ac

Amplicon sequencing detects mutations associated with pyrethroid resistance in Bemisia tabaci (Hemiptera: Aleyrodidae)

A feasibility trial of genomics‐based diagnosis detecting insecticide resistance of the diamondback moth

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