Multiple occupancy of nodules by nodulating rhizobia on field-grown soybeans with attendance ofSinorhizobiumspp.

Abstract

This study analyzed the phenotypic and genotypic characters of nodulating rhizobia isolated from two soybean cultivars, Kyushu 151 and Sachiyutaka, in the same field of the Yamaguchi Prefectural Technology Center of Agriculture and Forestry in Japan. The isolates were classified into groups using phenotypic characteristics, such as growth rate, color change on Bromothymol blue-containing yeast extract-mannitol agar (YMA) plates and colony morphology on YMA plates, and by genotypic characteristics, such as polymerase chain reaction-restriction fragment length polymorphism patterns of the 16S ribosomal RNA genes (16S rDNA) and the internal transcribed sequence (ITS) regions. In Kyushu 151, single phenotypic and genotypic groups were isolated from every nodule examined. In Sachiyutaka, plural strains belonging to distinct groups were obtained frequently from single nodules, indicating that multiple occupancy was established at high frequency. No fixed combination of the groups was found in the composition of multiple occupancy. An increase in the relative abundance of isolates belonging to Sinorhizobium fredii (Ensifer fredii) occurred concomitantly with the increase in the proportion of nodules with multiple occupancy. Nearly 60% of the isolates from Sachiyutaka belonged to S. fredii; 75% of them were obtained from nodules with multiple occupancy.

Culturable bacterial communities on leaf sheaths and panicles of rice plants in Japan

“…Particularly, although Sinorhizobium was also isolated from washings in the 2002 survey, this genus tended to be obtained specifically from macerates of the tissues based on the isolation frequencies, suggesting that the bacterial genus more often inhabit the endophytic environments of the leaf sheaths. Sinorhizobium is a leguminous bacterial genus and has been found in mostly Leguminosae plants and soil (Trabelsi et al 2009, Nguyen et al 2010), but several studies revealed that the bacterial genus artificially inoculated to rice plants colonized and showed growth promotion of the plants (Chi et al 2005(Chi et al , 2010. The current findings supported the inhabitancy and the potential roles of the bacterial genus on rice plants.…”