Multiple Lipid Compartments Slow Vesicle Contents Release in Lipases and Serum

Abstract

Unilamellar vesicles or "liposomes" are commonly used as simple cell models and as drug delivery vehicles. A major limitation of unilamellar liposomes in these applications has been premature contents release in physiological environments. This premature release is likely due to enzyme degradation or protein insertion into the liposome membrane, which significantly increases the bilayer permeability. Encapsulating unilamellar liposomes within a second bilayer to form multicompartment "vesosomes" extends contents retention by 2 orders of magnitude by preventing enzymes and/or proteins from reaching the interior bilayers. The multicompartment structure of the vesosome can also allow for independent optimization of the interior compartments and exterior bilayer; however, just the bilayer-within-a-bilayer structure of the vesosome is sufficient to increase drug retention from minutes to hours. The vesosome is a better mimic of eukaryotic cell structure and demonstrates the benefits of multiple internal bilayer-enclosed compartments.

Self-Assembled Multicompartment Liquid Crystalline Lipid Carriers for Protein, Peptide, and Nucleic Acid Drug Delivery

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17] The investigations, by means of time-resolved SAXS, of the dynamic topological transitions of selfassembled amphiphilic mixtures have allowed the discovery of lipid-based nanocarriers with tunable internal organizations and nanochannel sizes. [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32] The phase states of nonlamellar lipids, amphiphilic mixtures with lipopolymers or lipophilic conjugates, such as peptide surfactants, squalenoyl (SQ) derivatives, and acylated and peptidolipidyl-modified cyclodextrins, have been studied toward the design of novel controlled drug delivery nanocarriers with enhanced performance.…”

Application of nucleic acid–lipid conjugates for the programmable organisation of liposomal modules

“…These include careful assembly protocols for encapsulation of smaller vesicles within larger ones to create multicompartmental vesosomes [17][18][19], within layer-by-layer polymer shells [20][21][22], encapsulation of aqueous two phase systems within single liposomes [23][24][25] and association of lipid vesicles via site-specific ligand-receptor interactions such as biotin-avidin bonds [26][27][28]. Many of these approaches were discussed in a recent review [29].…”

High-intensity focused ultrasound-triggered nanoscale bubble-generating liposomes for efficient and safe tumor ablation under photoacoustic imaging monitoring

“…Liposomes formulated in the presence of aqueous PBS instead of ABC were used as the control (Lip-PBS). Using the method of phospholipid hydration followed by sequential extrusion and dialysis, 15 it was not difficult to incorporate ABC solution into liposomes, measure the diameter with a liposome extrusion apparatus, and remove the excess ABC solution with dialysate. Both Lip-ABC and Lip-PBS had nanoscale particle diameters and good uniformity (Table 1; Figure 1A and B) and would be able to pass through the tumor vascular endothelial space.…”