Multiple lectins as major proteins in the coelomic fluid of the acorn barnacle Megabalanus rosa

Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii

“…The presence of naturally occurring agglutinins in the hemolymph of several crustaceans has been well known since the beginning of the 20th century [34]. An evaluation of the literature revealed that purification of lectin from the hemolymph of crustaceans was most successful by affinity chromatography as it gave a higher fold of purification and percentage of recovery [20,24,27,35–39]. The lectin was purified from the hemolymph of P. jacquemontii by affinity chromatography.…”

“…The presence of a single lectin is a unique feature among brachyuran crabs [12,24,36]. The other crustaceans such as the barnacles [47,48], the freshwater prawn [26], marine prawn [50] and the lobsters [34,37], are distinctly marked by the presence of multiple lectins in its hemolymph. At present, the most popular belief is that lectins function primarily as recognition molecules [51].…”

Tn/T Specific Agglutinin from Estuarine Crab Scylla Serrata with Potent Mitogenic Activity on Mouse Splenocytes and Antiproliferative Effect on Hepatocellular Carcinoma (HepG2) Cell

“…This was confirmed by abolition of its inhibitory capacity by sialidase treatment and de-O-acetylation. Like the occurrence of multiple lectins in sponges, Axinella polypoides, 20 Pelina semitubulosa, 48 Halichondria okadii, 49 and blood clam Anadara granosa, 50,51 crustacean hemolymph contains multiple lectins 52,53 whose detailed carbohydrate specificity at the macromolecular level has not been worked out. The hemolymph of the edible crab Scylla serrata contains multiple lectins, the phenomenon was ascertained by its cross-adsorption test with erythrocytes (unpublished).We purified earlier a low molecular weight (5 kD) lectin scyllin from S. serrata collected from estuary of Gangetic delta that showed binding affinity to complex sialooligosaccharides of mammalian glycoproteins like fetuin, ceruloplasmin, porcine mucin, human glycophorin.…”

Βιοχημικη Μελετη Των Λεκτινων Της Αιμολεμφου Απο Τρια Ειδη Καρκινοειδων. Η Σημασια Τους Στην Αναγνωριση Κυτταρων Απο Θηλαστικα

“…Οι λεκτίνες στα καρκινοειδή εντοπίζονται στην αιμολέμφο των ζώων αυτών. Από τα κιοιπέδια έχει μελετηθεί το είδος Megabalanus rosa το οποίο φέρει πολλαπλές λεκτίνες οι οποίες αναγνωρίζουν γαλακτόζη [170,171]. Περισσότερα είδη έχουν μελετηθεί απο την τάξη των δεκαπόδων.…”