Multiple Infections with Viruses of the Family Tymoviridae in Czech Grapevines

Komínková, Marcela; Ben Mansour, Karima; Komínek, Petr; Brožová, Jana; Střalková, Radomíra

doi:10.3390/v16030343

Cited by 2 publications

(1 citation statement)

References 66 publications

(102 reference statements)

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“…Mixed infections with two or more viruses occur naturally in plants [9,45]. Infection with SCSMV, SCMV, and SrMV in sugarcane results in the manifestation of mosaic-like symptoms, which involve the appearance of irregular light and dark green patterns on leaves [8].…”

Section: Discussionmentioning

confidence: 99%

Mixed Mosaic Virus Infection and Its Impact on Photosynthetic Gene Expression in Sugarcane Fields in Indonesia

Neliana,

Sugiharto,

Soleha

et al. 2024

Preprint

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Sugarcane mosaic virus (SCMV) and sugarcane streak mosaic virus (SCSMV) are the primary viruses responsible for mosaic diseases in sugarcane. In this study, the mosaic viruses SCMV and SCSMV with the involvement of sorghum mosaic virus (SrMV) were surveyed from eight commercial sugarcane cultivars grown in fields at four locations in East Java, Indonesia. Sugarcane leaves with mosaic symptoms were assessed for disease incidence and severity, and symptomatic leaves were collected for analysis. The incidence and severity of mosaic symptoms were the highest in the PS881 and NX04 cultivars grown in Kediri compared to the other cultivars. RT-PCR analysis showed that SCSMV was present in all sugarcane cultivars and locations. Interestingly, double mixed infections with SCMV were found in PS881, PS882, and Cening, whereas triple mixed infections with SrMV were found in the PS881 cultivar. Phylogenetic analysis grouped SCSMV, SCMV, and SrMV together with isolates from Thailand (KP987832.1), Florida, USA (MT725538.1), and Agentina (EU189037.1). The expression of ascorbate peroxidase gene (Apx) was increased in the virus-infected sugarcane cultivars and prominently elevated in the triple mixed infection of PS881 cultivars. However, the catalase gene (Cat) was slightly upregulated in the mixed infection group. The chlorophyll content was significantly reduced in the leaves of PS58, PS881, PS882, NX04, and HW Merah, in parallel with the expression of the photosystem I gene (PsaA). The expression of photosynthetic genes, such as ribulose-1,5 bisphosphate carboxylase small sub-unit (RbcS), phosphoenolpyruvate carboxylase (Pepc), sucrose-phosphate synthase (Sps1), and PsaA, was significantly reduced and almost depleted in the triple mixed infection of PS881, except for Pepc expression, which remained approximately half of that in the control healthy sugarcane. The decrease in photosynthetic activity was confirmed by the decrease in ribulose-1,5 bisphosphate carboxylase (Rubisco) and PEPC proteins, as detected by immunoblot analysis. The decrease in photosynthetic parameters indicated that the mosaic viral infection substantially reduced the growth and yield of sugarcane.

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Section: Discussionmentioning

confidence: 99%

Mixed Mosaic Virus Infection and Its Impact on Photosynthetic Gene Expression in Sugarcane Fields in Indonesia

Neliana,

Sugiharto,

Soleha

et al. 2024

Preprint

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Alteration of Photosynthetic and Antioxidant Gene Expression in Sugarcane Infected by Multiple Mosaic Viruses

Neliana,

Soleha,

Suherman

et al. 2024

IJPB

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Sugarcane mosaic virus (SCMV), sugarcane streak mosaic virus (SCSMV), and sorghum mosaic virus (SrMV) are the causative pathogens of mosaic disease. This study aimed to identify mosaic virus infection and its impact on photosynthetic and antioxidant gene expression in eight commercial sugarcane cultivars grown on sugarcane plantations in East Java, Indonesia. The disease incidence and severity were observed in symptomatic leave samples, and then the virus was identified. A high incidence and severity of mosaic symptoms were observed in the PS881 and NX04 cultivars compared with the other cultivars. RT-PCR analysis detected SCSMV infection in all cultivars; double infections with SCSMV and SCMV in the PS881, PS882, and Cening cultivars; and triple infections with SCSMV, SCMV, and SrMV in the PS881 cultivar. Ascorbate peroxidase (Apx) expression was upregulated in all virus-infected cultivars and significantly increased in the triple-infected PS881 cultivar. However, catalase (Cat) expression was only slightly increased in the PS881 cultivar. The chlorophyll content was reduced, and the PsaA gene was downregulated in all cultivars. The expression of PsaA, RbcS, and Sps was significantly suppressed in the triple-infected PS881 cultivar. Moreover, the downregulation of both the RbcS and Pepc genes was concomitant with that of their protein levels.

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Multiple Infections with Viruses of the Family Tymoviridae in Czech Grapevines

Cited by 2 publications

References 66 publications

Mixed Mosaic Virus Infection and Its Impact on Photosynthetic Gene Expression in Sugarcane Fields in Indonesia

Mixed Mosaic Virus Infection and Its Impact on Photosynthetic Gene Expression in Sugarcane Fields in Indonesia

Alteration of Photosynthetic and Antioxidant Gene Expression in Sugarcane Infected by Multiple Mosaic Viruses

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