Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis

Morgan, Bruce; Ezeriņa, Daria; Amoako, Theresa N.E.; Riemer, Jan; Seedorf, Matthias; Dick, Tobias P.

doi:10.1038/nchembio.1142

Cited by 257 publications

(245 citation statements)

References 42 publications

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“…Measurement of S-glutathionylated proteins combined with depletion of reduced GSH and a decreased GSH:GSSG ratio are useful indicators of oxidative stress (76), especially in cancer (259). However, recently, the use of GSH levels to indicate whether a cell is under oxidative stress was challenged by a study showing that oxidized glutathione is transported to cellular vacuoles in yeast and mammalian cells (248).…”

Section: Measurement Of Antioxidantsmentioning

confidence: 99%

Nutritional Countermeasures Targeting Reactive Oxygen Species in Cancer: From Mechanisms to Biomarkers and Clinical Evidence

Samoylenko

Hossain²,

Mennerich³

et al. 2013

Antioxidants & Redox Signaling

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Reactive oxygen species (ROS) exert various biological effects and contribute to signaling events during physiological and pathological processes. Enhanced levels of ROS are highly associated with different tumors, a Western lifestyle, and a nutritional regime. The supplementation of food with traditional antioxidants was shown to be protective against cancer in a number of studies both in vitro and in vivo. However, recent largescale human trials in well-nourished populations did not confirm the beneficial role of antioxidants in cancer, whereas there is a well-established connection between longevity of several human populations and increased amount of antioxidants in their diets. Although our knowledge about ROS generators, ROS scavengers, and ROS signaling has improved, the knowledge about the direct link between nutrition, ROS levels, and cancer is limited. These limitations are partly due to lack of standardized reliable ROS measurement methods, easily usable biomarkers, knowledge of ROS action in cellular compartments, and individual genetic predispositions. The current review summarizes ROS formation due to nutrition with respect to macronutrients and antioxidant micronutrients in the context of cancer and discusses signaling mechanisms, used biomarkers, and its limitations along with large-scale human trials.

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Section: Measurement Of Antioxidantsmentioning

confidence: 99%

Nutritional Countermeasures Targeting Reactive Oxygen Species in Cancer: From Mechanisms to Biomarkers and Clinical Evidence

Samoylenko

Hossain²,

Mennerich³

et al. 2013

Antioxidants & Redox Signaling

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“…Increasing evidence suggests the subcellular partitioning of GSH and GSSG levels are of central importance for redox environment homeostasis specifically within organelles and the cytosol [30].…”

Section: A Rotifer Diet Disrupts Glutathione Homeostasis and Redox Pomentioning

confidence: 99%

“…E 0 ′ is the standard reduction potential at pH 7 and 25°C and was assumed to be À 0.240 V. The measurements are the average of whole larvae and do not take into account that the reduction potential varies between organs, and between organelles within the cells [16,30,31].…”

Section: Calculationsmentioning

confidence: 99%

Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae

Hamre¹,

Penglase²,

Edvardsen

et al. 2015

Free Radical Biology and Medicine

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a b s t r a c tThe growth and development of marine fish larvae fed copepods is superior to those fed rotifers, but the underlying molecular reasons for this are unclear. In the following study we compared the effects of such diets on redox regulation pathways during development of Atlantic cod (Gadus morhua) larvae. Cod larvae were fed a control diet of copepods or the typical rotifer/Artemia diet commonly used in commercial marine fish hatcheries, from first feeding until after metamorphosis. The oxidised and reduced glutathione levels, the redox potential, and the mRNA expression of 100 genes in redox system pathways were then compared between treatments during larval development. We found that rotifer/Artemia-fed cod larvae had lower levels of oxidised glutathione, a more reduced redox potential, and altered expression of approximately half of the redox system genes when compared to copepod-fed larvae. This rotifer/Artemia diet-induced differential regulation of the redox system was greatest during periods of suboptimal growth. Upregulation of the oxidative stress response transcription factor, nrf2, and NRF2 target genes in rotifer/Artemia fed larvae suggest this diet induced an NRF2-mediated oxidative stress response. Overall, the data demonstrate that nutritional intake plays a role in regulating the redox system in developing fish larvae. This may be a factor in dietary-induced differences observed in larval growth.

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“…Numerous cellular functions are closely correlated with small molecules, such as O 2 , 4 glucose, 5 glutathione, 6 NO, 7 CO 8 and H 2 O 2 , 9 for enzymatic co-factors, signaling and expression. Among them, H 2 O 2 is an important reactive oxygen species that can diffuse across cell membranes and lead to oxidative protein modification.…”

Section: Introductionmentioning

confidence: 99%

Bio-inspired porous antenna-like nanocube/nanowire heterostructure as ultra-sensitive cellular interfaces

Kong

Tang

et al. 2014

NPG Asia Mater

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In this study, an unconventional antenna-like heterostructure comprised of arrays of nanoporous Prussian blue (PB) nanocube heads/TiO 2 nanowire (NW) arms (PB-TiO 2 ) is developed for efficient three-dimensional interfacial sensing of small molecules and cellular activities. Inspired by insect tentacles, which are comprised of both target recognition and signal transduction units, one-dimensional TiO 2 NW arrays are grown, followed by selective growth of nanoporous PB nanocubes on the tips of the NW arrays. Due to their high selectivity and bioaffinity toward cells, long biostability under a cell culture adhesion condition (up to 108 h) is obtained, and with its inherent bio-mimetic enzymatic activity, the obtained nanoporous PB nanocubes (head segment) serve as robust substrates for site-selective cell adhesion and culture, which allows for sensitive detection of H 2 O 2 . Simultaneously, the single-crystalline TiO 2 NWs (arm segment) provide efficient charge transport for electrode substrates. Compared with PB-functionalized planar electrochemical interfaces, the PB-TiO 2 antenna NW biointerfaces exhibit a substantial enhancement in electrocatalytic activity and sensitivity for H 2 O 2 , which includes a low detection limit (B20 nM), broad detection range (10 À8 to 10 À5 M), short response time (B5 s) and long-term biocatalytic activity (up to 6 months). The direct cultivation of HeLa cells is demonstrated on the PB-TiO 2 antenna NW arrays, which are capable of sensitive electrochemical recording of cellular activity in real time, where the results suggest the uniqueness of the biomimic PB-TiO 2 antenna NWs for efficient cellular interfacing and molecular recognition.

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Multiple glutathione disulfide removal pathways mediate cytosolic redox homeostasis

Cited by 257 publications

References 42 publications

Nutritional Countermeasures Targeting Reactive Oxygen Species in Cancer: From Mechanisms to Biomarkers and Clinical Evidence

Nutritional Countermeasures Targeting Reactive Oxygen Species in Cancer: From Mechanisms to Biomarkers and Clinical Evidence

Diet affects the redox system in developing Atlantic cod (Gadus morhua) larvae

Bio-inspired porous antenna-like nanocube/nanowire heterostructure as ultra-sensitive cellular interfaces

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