Multiple Genome Sequences of Heteropolysaccharide-Forming Acetic Acid Bacteria

Brandt, Julia U.; Jakob, Frank; Geißler, Andreas; Behr, Jürgen; Vogel, Rudi F.

doi:10.1128/genomea.00185-17

Cited by 6 publications

(2 citation statements)

References 16 publications

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“…6 ? Supplementary File 2), an additional expressed b-fructosidase (WP077802344) (Brandt et al 2017;Jakob et al 2019) and/or the possible intrinsic levanase activity (Méndez-Lorenzo et al 2015) of the levansucrase at certain conditions. The presented experimental approach could hence be used to control and trigger the sizes of globular levan molecules.…”

Section: Discussionmentioning

confidence: 99%

Insights into the pH-dependent, extracellular sucrose utilization and concomitant levan formation by Gluconobacter albidus TMW 2.1191

Jakob

Gebrande

Bichler

et al. 2020

Antonie van Leeuwenhoek

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Many bacteria and archaea produce the polydisperse fructose polymer levan from sucrose upon biofilm formation via extracellular levansucrases (EC 2.4.1.10). We have investigated levansucraserelease and -activities as well as molecular size of the levan formed by the acetic acid bacterium Gluconobacter albidus TMW 2.1191 at varying environmental pH conditions to obtain insight in the ecological role of its constitutively expressed levansucrase and the produced levan. A buffer system was established enabling the recovery of levansucrasecontaining supernatants from preincubated cell suspensions at pH 4.3-pH 5.7. The enzyme solutions were used to produce levans at different pH values and sucrose concentrations. Finally, the amounts and size distributions of the produced levans as well as the corresponding levansucrase activities were determined and correlated with each other. The data revealed that the levansucrase was released into the environment independently of its substrate sucrose, and that more levansucrase was released at pH C 5.0.The glucose release and formation of high molecular weight levans ([ 3.5 kDa) from 0.1 M initial sucrose was comparable between pH * 4.3-5.7 using equal amounts of released levansucrase. Hence, this type of levansucrase appears to be structurally adapted to changes in the extracellular pH and to exhibit a similar total activity over a wide acidic pH range, while it produced higher amounts of larger levan molecules at higher production pH and sucrose concentrations. These findings indicate the physiological adaptation of G. albidus TMW 2.1191 to efficient colonisation of sucrose-rich habitats via released levansucrases despite changing extracellular pH conditions in course of acid formation.

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Section: Discussionmentioning

confidence: 99%

Insights into the pH-dependent, extracellular sucrose utilization and concomitant levan formation by Gluconobacter albidus TMW 2.1191

Jakob

Gebrande

Bichler

et al. 2020

Antonie van Leeuwenhoek

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“…Synthesis of levan was also confirmed for various strains of K. xylinus ( K. xylinus NCI 1005, NCIM 2526) [ 8 , 25 ]. N. chiangmaiensis (=BCC 15763 T = NBRC 101099 T ), isolated from red ginger flowers, is known to produce levan-like exopolysaccharides (on sucrose deficient medium) and heteropolysaccharides into their environments [ 30 , 31 ]. Previously, Jakob and co-workers quantified and characterized the levan produced by strains of the species G. frateurii , G. cerinus , N. chiangmaiensis , and Ko.…”

Section: Introductionmentioning

confidence: 99%

Candidate Acetic Acid Bacteria Strains for Levan Production

et al. 2022

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In this study, twelve strains of acetic acid bacteria (AAB) belonging to five different genera were tested for their ability to produce levan, at 70 and 250 g/L of sucrose concentration, respectively. The fructan produced by the bacterial strains was characterized as levan by NMR spectroscopy. Most of the strains produced levan, highlighting intra- and inter-species variability. High yield was observed for Neoasaia chiangmaiensis NBRC 101099 T, Kozakia baliensis DSM 14400 T and Gluconobacter cerinus DSM 9533 T at 70 g/L of sucrose. A 12-fold increase was observed for N. chiangmaiensis NBRC 101099 T at 250 g/L of sucrose concentration. Levan production was found to be affected by glucose accumulation and pH reduction, especially in Ko. baliensis DSM 14400 T. All the Gluconobacter strains showed a negative correlation with the increase in sucrose concentration. Among strains of Komagataeibacter genus, no clear effect of sucrose on levan yield was found. Results obtained in this study highlighted the differences in levan yield among AAB strains and showed interdependence between culture conditions, carbon source utilization, and time of incubation. On the contrary, the levan yield was not always related to the sucrose concentration.

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Genome sequences and description of novel exopolysaccharides producing species Komagataeibacter pomaceti sp. nov. and reclassification of Komagataeibacter kombuchae (Dutta and Gachhui 2007) Yamada et al., 2013 as a later heterotypic synonym of Komagataeibacter hansenii (Gosselé et al. 1983) Yamada et al., 2013

Škraban

Cleenwerck

Vandamme

et al. 2018

Systematic and Applied Microbiology

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Multiple Genome Sequences of Heteropolysaccharide-Forming Acetic Acid Bacteria

Cited by 6 publications

References 16 publications

Insights into the pH-dependent, extracellular sucrose utilization and concomitant levan formation by Gluconobacter albidus TMW 2.1191

Insights into the pH-dependent, extracellular sucrose utilization and concomitant levan formation by Gluconobacter albidus TMW 2.1191

Candidate Acetic Acid Bacteria Strains for Levan Production

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