Multiple genetic copy number alterations in oral squamous cell carcinoma: study of MYC
, TP53
, CCDN1, EGFR
 and ERBB2
 status in primary and metastatic tumours

Martín‐Ezquerra, Gemma; Salgado, Rocío; Toll, Agustí; Gilaberte, Montserrat; Baró, Teresa; Quitllet, Francesc Alameda; Yébenes, Mireia; Solé, Françesc; García-Muret, M. Pilar; Espinet, Blanca

doi:10.1111/j.1365-2133.2010.09947.x

Cited by 43 publications

(39 citation statements)

References 35 publications

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“…This high degree of identity is reflected by the signal obtained for this antibody in our experiments (Fig 1). The qualitative expression of c-Myc is increased in 80% of oral squamous cell carcinoma in a South Indian population [51] and it was reported that there is an amplification in the gene copy number in both c-Myc and CD1 in human oral carcinomas [52]. c-Myc is also seen to be upregulated in both multiple myeloma and colon cancer as part of the interleukin signalling pathway [32, 33].…”

Section: Discussionmentioning

confidence: 99%

Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma

et al. 2016

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Feline oral squamous cell carcinoma (FOSCC) is an aggressive neoplasm in cats. Little is known about the possible molecular mechanisms that may be involved in the initiation, maintenance and progression of FOSCC. Wnt signalling is critical in development and disease, including many mammalian cancers. In this study, we have investigated the expression of Wnt signalling related proteins using quantitative immunohistochemical techniques on tissue arrays. We constructed tissue arrays with 58 individual replicate tissue samples. We tested for the expression of four key Wnt/ß-catenin transcription targets, namely Cyclin D1 (CCND1 or CD1), FRA1, c-Myc and MMP7. All antibodies showed cross reactivity in feline tissue except MMP7. Quantitative immunohistochemical analysis of single proteins (expressed as area fraction / amount of tissue for normal vs tumor, mean ± SE) showed that the expression of CD1 (3.9 ± 0.5 vs 12.2 ± 0.9), FRA1 (5.5 ± 0.6 vs 16.8 ± 1.1) and c-Myc (5.4 ± 0.5 vs 12.5 ± 0.9) was increased in FOSCC tissue by 2.3 to 3 fold compared to normal controls (p<0.0001). By using a multilabel, quantitative fluorophore technique we further investigated if the co-localization of these proteins (all transcription factors) with each other and in the nucleus (stained with 4',6-diamidino-2-phenylindole, DAPI) was altered in FOSCC compared to normal tissue. The global intersection coefficients, a measure of the proximity of two fluorophore labeled entities, showed that there was a significant change (p < 0.01) in the co-localization for all permutations (e.g. CD1/FRA1 etc), except for the nuclear localization of CD1. Our results show that putative targets of Wnt signalling transcription are up-regulated in FOSCC with alterations in the co-localization of these proteins and could serve as a useful marker for the disease.

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Section: Discussionmentioning

confidence: 99%

Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma

et al. 2016

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“…Several studies have analyzed c-myc expression in this type of tumor showing different results, with an average positivity of 41.28% (2.4-75%) between the different studies (6,(26)(27)(28)(29)(30)(31)(32)(33)(34). The results are extremely contradictory, as well as the variation in the quantification methods.…”

Section: Discussionmentioning

confidence: 99%

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

et al. 2014

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Abstract.Myc genes are a family of proto-oncogenes whose proteins are implicated in the regulation of cell proliferation, differentiation and apoptosis, and in regulating the activity of genes involved in cell division. The aim of the present study was to establish a quantitative description of the expression of c-myc and evaluate its relationship with other clinical and prognostic factors, as well as to establish a multivariate survival prediction model. This is a retrospective study of 68 patients diagnosed with oral squamous cell carcinoma (OSCC). We constructed a tissue microarray for investigating the expression of c-myc by immunohistochemistry. Statistical analyses were carried out, and a multivariate model that predicts survival was established. The average expression of c-myc was 50.32 (SD, 26.05) with a range from 6.60 to 99.

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“…11,12 They seem to have a relevant role in the biological behavior of several carcinomas. Frequent integration of HPV at 8q24 in cervical carcinoma cell lines has also been reported.…”

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confidence: 99%

MYC Copy Number Gains are Associated with Poor Outcome in Penile Squamous Cell Carcinoma

Masferrer

Ferrándiz-Pulido

Lloveras³

et al. 2012

Journal of Urology

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Multiple genetic copy number alterations in oral squamous cell carcinoma: study of MYC , TP53 , CCDN1, EGFR and ERBB2 status in primary and metastatic tumours

Cited by 43 publications

References 35 publications

Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma

Quantitative Expression and Co-Localization of Wnt Signalling Related Proteins in Feline Squamous Cell Carcinoma

Quantitative determination of c-myc facilitates the assessment of prognosis of OSCC patients

MYC Copy Number Gains are Associated with Poor Outcome in Penile Squamous Cell Carcinoma

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