Multiple Forms of<i>β</i><sub>2</sub>-Microglobulin in Human Colostrum

Tanahashi, Nobuyasu; Watanabe, Yutaka; Yamada, Fujizo

doi:10.1080/00021369.1979.10863606

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“…Our observation, that an average of only one methylamine molecule was incorporated per (-+2-m monomer in the isotope incorporation experiment, suggests the presence of few (perhaps only one) appropriate glutamine residue(s) for the enzyme in the sequence . Also, deamination Of ,82 -M may occur during the preparation of the protein or in vivo (there have been reports showing isomeric forms of /32-m [3,14]), and eliminate the appropriate glutamine residue(s) of #2-m from some of the molecules. Second, the conformation of ,82-m (5) may allow the Tgase-catalyzed formation of intramolecular e-(y-glutamyl) lysine crosslinks .…”

Section: Resultsmentioning

confidence: 99%

Human beta 2-microglobulin is a substrate of tissue transglutaminase: polymerization in solution and on the cell surface.

Fésüs

Falus

Erdei

et al. 1981

The Journal of Cell Biology

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Incubation of purified human beta 2-microglobulin (B2-m) with tissue transglutaminase (Tgase) resulted in the formation of high molecular weight polymers revealed by sodium dodecyl sulfate polyacrylamide gel electrophoresis. In the presence of 30 mM [14C]methylamine, the polymer formation was prevented, but incorporation of methylamine into beta 2-m (equal to 1 methylamine per 1 molecule) could be observed. From the sheddings of peripheral blood mononuclear cells occurring in the presence of Tgase, it is apparent that anti-beta 2-m immunoadsorbent removed, in addition to human leukocyte antigen (HLA) and beta 2-m, some other proteins. The enzyme could incorporate [14C]methylamine into beta 2-m of the shedding cells. On addition of rabbit anti-human beta 2-m antibody, followed by fluoresceine-labeled goat anti-rabbit IgG antibody to human mononuclear blood cells, the otherwise homogeneous distribution of fluorescence turned into spots and patches on cells previously incubated with Tgase or Ca2+-ionophore A23187.

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Section: Resultsmentioning

confidence: 99%