Multiple forms of extracellular superoxide dismutase in needles, stem tissues and seedlings of Scots pine

Schinkel, Helga; Streller, Steffen; Wingsle, Gunnar

doi:10.1093/jxb/49.323.931

Cited by 18 publications

(8 citation statements)

References 32 publications

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“…2(B) and 5(A)), has been widely documented in plants in response to abiotic or biotic stresses (Mika et al, 2004) and was in agreement with the increase in PA observed during treatment with copper found in previous studies conducted in our lab (Navari-Izzo et al, 2006). Extracellular SOD could be in close association with the NADPH oxidase complex and could catalyse the dismutation to H 2 O 2 determining the oxidative burst (Schinkel et al, 1998). Maximum production of H 2 O 2 occurred within the first 15 min of treatment (Fig.…”

Section: Article In Presssupporting

confidence: 89%

Early production of activated oxygen species in root apoplast of wheat following copper excess

Sgherri

Quartacci

Navari-Izzo

2007

Journal of Plant Physiology

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Section: Article In Presssupporting

confidence: 89%

Early production of activated oxygen species in root apoplast of wheat following copper excess

Sgherri

Quartacci

Navari-Izzo

2007

Journal of Plant Physiology

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“…Values of about 200% recovered activity of the cytosolic PEPC were found in the 82 000 g supernatant fractions after differential centrifugation ( Tables 1 and 2), indicating a high degree of correlation between the supernatant fractions and the cytosolic compartment ( Winter et al 1982 ; Stitt et al 1989 ; Schinkel, Streller & Winsgle 1998). Taken together, these results show that the purity of the 82 000 g supernatant fractions is about 70–80%, which we consider sufficient and more suitable than crude extracts to make a meaningful studies of the soluble antioxidant enzymes.…”

Section: Discussionmentioning

confidence: 97%

Tolerance of pea (Pisum sativum L.) to long‐term salt stress is associated with induction of antioxidant defences

Hernández

Jiménez

Mullineaux

et al. 2000

Plant Cell & Environment

760

480

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Using two cultivars of Pisum sativum L. with different sensitivity to NaCl, the effect of long-term (15 d) NaCl (70 mM) treatments on the activity and expression of the foliar ascorbate-glutathione cycle enzymes, superoxide dismutase isozymes and their mRNAs was evaluated and related to their ascorbate and glutathione contents. High-speed supernatant (soluble) fractions, enriched for cytosolic components of the antioxidant system, were used. In this fraction from the NaCl-tolerant variety (cv Granada), the activities of ascorbate peroxidase (APX), glutathione reductase (GR), monodehydroascorbate reductase (MDHAR), Mn-superoxide dismutase (Mn-SOD) and dehydroascorbate reductase (DHAR) increased, while CuZn-SOD activity remained constant. In the NaClsensitive plants (cv Challis), salinity did not produce significant changes in APX, MDHAR and GR activities. Only DHAR activity was induced in cv Challis, whereas soluble CuZn-SOD activity decreased by about 35%. Total ascorbate and glutathione contents decreased in both cultivars, but the decline was greater in NaCl-sensitive plants. This difference between the two cultivars was more pronounced when the transcript levels of some these enzymes were examined. Transcript levels for mitochondrial Mn-SOD, chloroplastic CuZn-SOD and phospholipid hydroperoxide glutathione peroxidase (PHGPX), cytosolic GR and APX were strongly induced in the NaCl-tolerant variety but not in the NaCl-sensitive variety. These data strongly suggest that induction of antioxidant defences is at least one component of the tolerance mechanism of peas to long-term salt-stress.

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“…2002). On the other hand, even though representing a small percentage of total superoxide dismutase (SOD), an apoplastic Cu–Zn SOD could scavenge, at least in part, the superoxide ion (Schinkel et al. 1998; Srivastava 1999).…”

Section: Introductionmentioning

confidence: 99%

Metabolic‐dependent changes in plant cell redox power after ozone exposure

et al. 2009

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The tropospheric level of the phytotoxic air pollutant ozone has increased considerably during the last century, and is expected to continue to rise. Long-term exposure of higher plants to low ozone concentrations affects biochemical processes prior to any visible symptoms of injury. The current critical level of ozone used to determine the threshold for damaging plants (biomass loss) is still based on the seasonal sum of the external concentration above 40 nl.l(-1) (AOT40). Taking into account stomatal conductance and the internal capacity of leaf defences, a more relevant concept should be based upon the 'effective ozone flux', the balance between the stomatal flux and the intensity of cellular detoxification. The large decrease in the Rubisco/PEPc ratio reflects photosynthetic damage from ozone, and a large increase in activity of cytosolic PEPc, which allows increased malate production. Although the direct detoxification of ozone (and ROS produced from its decomposition) is carried out primarily by cell wall ascorbate, the existing level of this antioxidant is not sufficient to indicate the degree of cell sensitivity. In order to regenerate ascorbate, NAD(P)H is needed as the primary supplier of reducing power. It is hypothesised that increased activity of the catabolic pathways and associated shunts (glucose-6-phosphate dehydrogenase, NADP-dependent glyceraldehyde-3-phosphate dehydrogenase, isocitrate dehydrogenase and malic enzyme) can provide sufficient NAD(P)H to maintain intracellular detoxification. Thus, measurement of the level of redox power would contribute to determination of the 'effective ozone dose', serving ultimately to improve the ozone risk index for higher plants.

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Multiple forms of extracellular superoxide dismutase in needles, stem tissues and seedlings of Scots pine

Cited by 18 publications

References 32 publications

Early production of activated oxygen species in root apoplast of wheat following copper excess

Early production of activated oxygen species in root apoplast of wheat following copper excess

Tolerance of pea (Pisum sativum L.) to long‐term salt stress is associated with induction of antioxidant defences

Metabolic‐dependent changes in plant cell redox power after ozone exposure

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