Multiple fluorescence immunoassay for the simultaneous detection of Zearalenone and Ochratoxin A

Zhou, Jingming; Liu, Zhanxiang; Yang, Qingbao; Qian, Wenjing; Chen, Yumei; Qi, Yanhua; Wang, Aiping

doi:10.1016/j.ab.2021.114288

Cited by 18 publications

(3 citation statements)

References 47 publications

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“…The supernatant was isolated after filtration using medium‐speed quantitative filter paper. Then, the filtrate was diluted 20‐fold with PBS (Zhou, Liu, et al, 2021). The pH of supernatant was adjusted to 7.0 with HCl (0.1 mol/L).…”

Section: Methodsmentioning

confidence: 99%

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Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

Zhou

Wang

et al. 2022

Journal of Food Safety

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Zearalenone (ZEN) is one of the most common mycotoxin contaminants worldwide. In this study, a phage-based direct competitive fluorescence immunosorbent assay (P-dcFLISA) was developed for the detection of ZEN. In this P-dcFLISA, phage mimotope was used to replace chemically synthesized antigens to improve the safety of experiments. Furthermore, ZnCdSe/ZnS (core/shell) quantum dots (QDs) as fluorescent labeling material replaced chromogenic substrate, which are used in traditional ELISA. The 50% inhibition value (IC 50 ) of P-dcFLISA was 0.301 ng/ml, which was approximately 12-fold lower than that of phage-based indirect competitive enzyme-linked immunosorbent assay (P-icELISA). The limit of detection (LOD) of P-dcFLISA was 0.023 ng/ml and the detection range was 0.060-1.531 ng/ml. In the added recovery assay, recovery rate was 93.18-105.52%, with the coefficient variation (CV) ranging from 8.39% to 10.55%. The establishment of P-icFLISA not only save time, but also avoid the use of mycotoxins in the detection process, providing a safe method for the detection of mycotoxins. In conclusion, phage-based fluorescence immunoassay method has great potential application in ZEN detection and agricultural monitoring.

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Section: Methodsmentioning

confidence: 99%

“…Although the developed immunoassay methods are excellent, they are used for detection of ZEN involving the use of ZEN itself, including the free form and the bound form (Li & Deng, 2016; Zhou, Liu, et al, 2021). Mycotoxin standards are also expensive.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

Zhou

Wang

et al. 2022

Journal of Food Safety

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“…Ochratoxin A (OTA) was one of the most toxic ochratoxins, which had universally contaminated for the coffee, wine, cereal, and their by‐products (Akgonullu et al., 2022; Ngolong et al., 2020). The excessive OTA might cause the hepatotoxicity, nephrotoxicity, immunotoxicity, and teratogenic and carcinogenic risk (Zhou et al., 2021). OTA had always been concerned by governments and consumers worldwide, which was divided into grade 2B for carcinogens by the International Agency for Research on Cancer (IARC) (Li et al., 2022).…”

Section: Introductionmentioning

confidence: 99%

DNA‐bridged double gold nanoparticles‐based immunochromatography for dual‐mode detection of ochratoxin A

Qiu,

Liu,

Mao

et al. 2023

Journal of Food Science

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A novel immunochromatography strip (ICS) based on the DNA‐bridged double gold nanoparticles was established and evaluated for the dual‐mode detection of ochratoxin A (OTA). For this purpose, the streptavidin was coupled with the big size of gold nanoparticle (40 nm, AuNP), the OTA monoclonal antibody and biotinylated DNA‐SH were simultaneously immobilized on the small size of AuNP (20 nm), and then the enhanced ICS was self‐assembled and evaluated. The dual‐mode detection of semi‐quantification and quantification had been achieved and performed by the proposed ICS. The LOD for semi‐quantification (semi‐Q‐LOD) was 0.06 ng/mL by the directly naked eye (eightfold enhanced than conventional ICS). By the on‐site reader, the LOD for quantification (Q‐LOD) was 0.03 ng/mL (threefold enhanced), with the detection range between 0.03 and 1.2 ng/mL. The specificity, reliability, and practicability had been well represented. Furthermore, the OTA‐positive results of the enhanced ICS method correlated well with those obtained by the referenced HPLC–MS/MS for the market samples. This study provided a new ICS pattern of semi‐quantification and quantification for OTA contamination, which could be used as a valuable reference for improving the ICS technology and enhancing the sensitivity.

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Ultrasensitive electrochemical immunosensor based on Fe3O4@g-C3N4 nanocomposites for detection of TCM root-rot early warning biomarker - zearalenone

Sun,

Dang,

Gao

et al. 2024

J Solid State Electrochem

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Multiple fluorescence immunoassay for the simultaneous detection of Zearalenone and Ochratoxin A

Cited by 18 publications

References 47 publications

Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

Fluorescence immunoassay based on phage mimotope for nontoxic detection of Zearalenone in maize

DNA‐bridged double gold nanoparticles‐based immunochromatography for dual‐mode detection of ochratoxin A

Ultrasensitive electrochemical immunosensor based on Fe3O4@g-C3N4 nanocomposites for detection of TCM root-rot early warning biomarker - zearalenone

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