Multiple enzyme linked immunosorbent assay system on a capillary-assembled microchip integrating valving and immuno-reaction functions

Henares, Terence G.; Funano, Shun‐ichi; Terabe, Shigeru; Mizutani, Fumio; Sekizawa, Ryuichi; Hisamoto, Hideaki

doi:10.1016/j.aca.2007.02.060

Cited by 41 publications

(30 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Thus, the treated microchannels can provide a wider dynamic range and a lower detection limit (20 pg/mL) than the conventional 96-well microtiter plate. It is also worthwhile to note that the detection limit of 20 pg/mL achieved in the treated microchannels is much lower than that obtained in other microchip-based ELISA immunoassays which range from several hundred pg/mL to ng/mL (Herrmann et al, 2007;Henares et al, 2007;Jang et al, 2006;Kakuta et al, 2006;Yu et al, 2005).…”

Section: ããmentioning

confidence: 96%

“…Since the surface area in the microdevices is very small, the total bioactivity of antibodies will predominantly rely on the immunoreaction activity of immobilized antibody (Bai et al, 2006). So, several antibody-polymer immobilization strategies have been developed to improve the sensitivity of ELISA in polymeric microdevices, ranging from antibody passive adsorption or covalent immobilization on microbead/gel (Herrmann et al, 2007;Kakuta et al, 2006;Sato et al, 2004), covalent crosslinking via linkage reagents, (Henares et al, 2007;Jang et al, 2006;Yu et al, 2005), to covalent immobilization by a polymer linker . However, these methods either need special manipulation devices (Herrmann et al, 2007) or involve several intermediates and environmentally unfriendly solvents (Jang et al, 2006;Kakuta et al, 2006;Yu et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

Yuan

Lee

2008

Biotech & Bioengineering

View full text Add to dashboard Cite

Highly efficient antibody immobilization is extremely crucial for the development of high-performance polymeric microdevices for enzyme-linked immunosorbent assay (ELISA). In this article, a site-selective tyrosinase (TR)-catalyzed protein A strategy for antibody immobilization was developed to enhance the sensitivity of ELISA in poly-(methyl methacrylate) (PMMA) microchannels for interferon-gamma (IFN-gamma) assay. To effectively immobilize the target antibodies, oxygen plasma was first used to activate the inert PMMA. This is followed by poly(ethyleneimine) (PEI) coating, an amine-containing functional polymer. For comparison, protein A was also immobilized through the commonly used amine-glutaraldehyde (GA) chemistry. Oxygen plasma treatment effectively increased the amount of PEI attachment and subsequent binding efficiency of the primary antibody. The antibody immobilized via TR-catalyzed protein A was able to provide much better specific antigen capture efficiency than GA chemistry due to the optimal spacing and orientation. Consequently, by using this new method, the detection signal and the signal-to-noise ratio of the ELISA immunoassay in microdevices were all significantly improved. In comparison to the standard assay carried out in the 96-well microtiter plate, the treated microchannels exhibited a broader detection range and a shorter detection time. And the detection limit was also decreased to 20 pg/mL, much lower than that obtained in other microdevices.

show abstract

Section: ããmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

Yuan

Lee

2008

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…Sixty-four simultaneous assays were successfully demonstrated. A new approach for integration of various MUOs using a capillary-assembled microchip is one of the promising methods for general and multiplexed immunoassays [93]. chips has elicited enormous interest because the amount of original matter available for genetic analysis is often limited.…”

Section: Integration Of Immunoassays On Microchipsmentioning

confidence: 99%

Microflow Systems for Chemical Synthesis and Analysis: Approaches to Full Integration of Chemical Process

Mawatari¹,

Kazoe²,

Aota³

et al. 2011

J Flow Chem

View full text Add to dashboard Cite

Integrated microchemical systems on microchips, which are based on continuous microflows, are expected to become important tools for analysis and chemical synthesis applications for biological sciences and technologies. For these purposes, general integration concepts have been developed, including microunit operations (MUOs) and continuousflow chemical processing (CFCP) to create fully functional systems for various chemical processing applications. The general methodology has enabled analysis, synthesis, and fabrication of chemical systems on microchips, and these microsystems have demonstrated superior performance (e.g., rapid, simple, easy operation, and highly efficient processing) compared to conventional methodologies. Microchemical technology has now entered the phase of practical application. In this review, we discuss the methods for integration of continuous flow-based chemical process on microchips, relevant technologies, and applications.

show abstract

“…44 Moreover, simultaneous multiple capillary ELISA was carried out in a single CAs-CHIP system with valving operation. 45 Using a thermal lens microscope, simultaneous detection of human, chicken, and goat antigens was measured. The on-chip CAs-CHIP results with valving function were determined to be comparable with the results of off-chip capillary immunosensor detection (Fig.…”

Section: ·1·4 Immunoassaymentioning

confidence: 99%

Advancements in Capillary-Assembled Microchip (CAs-CHIP) Development for Multiple Analyte Sensing and Microchip Electrophoresis

et al. 2014

Self Cite

View full text Add to dashboard Cite

Multiple enzyme linked immunosorbent assay system on a capillary-assembled microchip integrating valving and immuno-reaction functions

Cited by 41 publications

References 19 publications

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

Protein A‐based antibody immobilization onto polymeric microdevices for enhanced sensitivity of enzyme‐linked immunosorbent assay

Microflow Systems for Chemical Synthesis and Analysis: Approaches to Full Integration of Chemical Process

Advancements in Capillary-Assembled Microchip (CAs-CHIP) Development for Multiple Analyte Sensing and Microchip Electrophoresis

Contact Info

Product

Resources

About