Multiple enzymatic profiles of Vibrio parahaemolyticus strains isolated from oysters

Costa, Renata Albuquerque; Amorim, Ludimila M.M. Conde; Araújo, Rayza Lima; Vieira, Regine Helena Silva dos Fernandes

doi:10.1016/s0325-7541(13)70035-x

Cited by 16 publications

(23 citation statements)

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“…Four tests of elastase, lipase, hemolysin and chitinase showed no activity for 100% of collected isolates both AHPND and non -AHPND ones. For elastase and lipase, it is somewhat in agreement with previous study, which did not detect elastase but lipase (80%) in V. paraheamolyticus (Costa et al, 2013). The variation of lipase expression in the two studies may due to the origin of V. paraheamolyticus isolates, one from Coptodisca rhizophorae oyster from Brazil, the other from Penaeus monodon shrimp (Costa et al, 2013).…”

Section: Resultssupporting

confidence: 91%

“…Vibrio species such as Vibrio cholera, Vibrio vulnificus and V. parahaemolyticus have been carried out (Oliver et al, 1986;Vermelho et al, 1996;Fiore et al, 1997;Kadokura et al, 2007;Costa et al, 2013). However, in Vietnam, there has not been any similar project on V. parahaemolyticus until now.…”

Section: Studies On Production Of Extracellular Enzymes Ofmentioning

confidence: 99%

“…There is evidence that V. parahaemolyticus harbors other extracellular enzymes that would contribute to its virulence (Kadokura et al, 2007;Costa et al, 2013). Various extracellular factors including enterotoxin, hemolysin, cytotoxin, protease, collagenase, phospholipase, siderophore, and hemagglutinin are known to be produced by pathogenic Vibrios (Janda et al, 1988;Costa et al, 2013;Bunpa et al, 2015). Among them, the most common virulence types are extracellular enzymes to attack cell membrane (Vergis et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Evaluating the Anti-Bacterial Activity of Mycelium Extract From Different Wood-Decay-Fungus Species Collected in Southern Vietnam

Hoa¹,

Vu²,

Linh³

2018

Vietnam J. Biotechnol.

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The antibacterial activity of twelve different wood-decay fungal isolated in Southern Vietnam was investigated to find out the new potential antibiotics produced by macro fungi. The antibacterial activity of the collected fungal isolates was tested by three screening methods against seven different strains of bacteria: Bacillus subtilis, Enterococcus faecalis, Staphylococcus aureus, Escherichia coli, Vibrio parahaemolyticus, Vibrio cholerae, and Salmonella typhi. The screening test were performed at first with mycelium grew on surface of agar medium similar to a disc diffusion test, next by an extracellular metabolite test by flood-plate method, and finally with an intracellular metabolite test using grounded mycelium fluid. To determine the activity of metabolites produced in the mycelium, the fungal isolates were grown on culture substrates to promote mycelium formation. Antibacterial activity of the selected fungi was confirmed by disc diffusion method using crude extract of the mycelium harvested from the substrate cultures. Four fungal species identified with strong antibacterial compounds produced by their mycelium including Pycnoporus sanguineus, Flavodon flavus, Fomitopsis ostreiformis and Meripilus giganteus. Crude extract of Pycnoporus sanguineus showed the strongest antibacterial activity, with 7/7 bacterial strains inhibited. Fomitopsis ostreiformis antibacterial activity was recognized with the ability to inhibit 6/7 bacterial strains. Flavodon flavus and Meripilus giganteus showed weaker activity than other two species, with only 4/7 strains inhibited. The fungal isolates identified with antibacterial activity discovered in this study can be potential candidates for the new source of antibiotic to fight against new generations of antibiotic resistance bacteria in the future.

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Section: Resultssupporting

confidence: 91%

Section: Studies On Production Of Extracellular Enzymes Ofmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Evaluating the Anti-Bacterial Activity of Mycelium Extract From Different Wood-Decay-Fungus Species Collected in Southern Vietnam

Hoa¹,

Vu²,

Linh³

2018

Vietnam J. Biotechnol.

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“…It has been known that the ability of Vibrio spp. to cause gastroenteritis is associated with the haemolysin production (Costa et al, ). Both V. parahaemolyticus and V. diabolicus were negative, and V. fluvialis (71%), V. alginolyticus (22%) , V. anguillarum (50%) and V. aestuarianus (100%) were positive for haemolysis.…”

Section: Resultsmentioning

confidence: 99%

“…such as V. cholerae and V. fluvialis are also known to harbor specific virulence-related genes (Chakraborty et al, 2000;Liang, Cui, Du, Kan, & Liang, 2013). In addition to the haemolysins, there are various extracellular enzymes such as lipase, protease, gelatinase, phospho-lipase, amylase, and DNase produced by vibrios which involve in their pathogenicity and infection (Costa, Amorim, Araújo, & dos Fernandes Vieira, 2013).…”

Section: Introductionmentioning

confidence: 99%

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Silva

Hossain

Dahanayake

et al. 2019

Journal of Food Safety

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We report the prevalence and characterization of Vibrio spp. isolated from marketed Yesso scallop (Patinopecten yessoensis) in Korea. A total of 30 isolates including, V. parahaemolyticus (n = 2), V. alginolyticus (n = 9), V. fluvialis (n = 7), V. diabolicus (n = 7), V. anguillarum (n = 4) and V. aestuarianus (n = 1) were isolated and identified. The phenotypic pathogenicity tests demonstrated that, 18 (60%), 21 (70%), 18 (60%), 7 (23%), 22 (73%), 21 (70%), 9 (30%), and 11 (33%) of the isolates were positive for DNase, protease, gelatinase, lipase, phospho‐lipase, amylase, slime production, and haemolysis, respectively. PCR assays revealed the prevalence of toxR, tlh, VAC, vfh, hupO, and VPI genes among the isolates with varying combinations. A close genetic affinity among V. alginolyticus and V. diabolicus strains was observed. Also the virulence genes specific to one Vibrio species were detected among other species as well. In addition, 29/30 (97%) isolates were multidrug resistant, while higher resistance rates were shown for ampicillin, colistin, vancomycin, and cephalothin. The results imply that the scallops in Korean markets harbor Vibrio spp., which are potentially virulent and multidrug resistant, thus their public health implications should not be underrated. Practical applications For many decades, vibrios are known for its importance in seafoodborne illnesses. Yesso scallop is the most popular and extensively cultured scallop variety in Korea. Therefore, we sought to assess the marketed fresh Yesso scallops for the prevalence and molecular characterization of Vibrio species. A total of 30 strains were isolated and identified by a series of biochemical tests, subsequent gyrB gene sequencing and phylogenetic analyses. Six Vibrio spp. were identified with V. alginolyticus as the most prevalent. Interestingly, V. alginolyticus was genetically similar to V. diabolicus. Besides, the virulence genes specific to V. alginolyticus and V. parahaemolyticus were observed in other species as well. It suggests that the detection of the species‐specific genes does not ensure the correct identification of pathogenic vibrios. Further, the occurrence of V. parahaemolyticus‐specific virulence genes in other Vibrio spp. potentially complicates the correct tracking of V. parahaemolyticus infections. In addition, 73% of these Vibrio spp. isolates showed multiple antibiotic resistance (MAR) indices higher than 0.2, which signifies their high risk of infection. Collectively, these results provide important evidence that not only the well‐known pathogenic vibrios like V. parahaemolyticus, but also other Vibrio spp. can act alike because of their similar characteristics.

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Identification and analysis of pathogenic bacteria causing outbreaks in Indian major carp aquaculture of Tripura

et al. 2023

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ObjectiveThe objective of this study was to investigate bacterial disease outbreaks in Indian major carp from aquaculture systems in Tripura, India, and identify the bacterial species associated with these outbreaks.MethodsA 3‐year surveillance was conducted in 8 districts of Tripura, during which 9 bacterial disease outbreaks were recorded. Fourteen bacterial strains isolated from diseased Indian major carp were selected and identified using phenotypic, molecular (16S rRNA gene), and phylogenetic analysis. In‐vitro pathogenicity studies were performed to assess the potential pathogenicity of the isolated bacteria.ResultsThe selected isolated strains were preliminarily identified under the genera Aeromonas (9 isolates), Acinetobacter (1 isolate), Citrobacter (3 isolates), and Pseudomonas (1 isolate). Molecular and phylogenetic analysis confirmed the species of the isolated bacteria, including Aeromonas jandaei (with strains COF_AHE09 and COF_AHE61), Aeromonas veronii (with strains COF_AHE13, COF_AHE52, COF_AHE55, COF_AHE56, and COF_AHE62), Aeromonas hydrophila (with strains COF_AHE51 and COF_AHE58), Acinetobacter pittii (with strain COF_AHE14), Citrobacter freundii (with strains COF_AHE20, COF_AHE57, and COF_AHE59), and Pseudomonas aeruginosa (with strain COF_AHE54). Behavioral and clinical signs observed in the diseased fish, such as lethargy, skin hemorrhaging, ulcers, fin and tail rot, pop eye, distended abdomen, scale loss, and skin discoloration, indicated the presence of bacterial septicemia. The in‐vitro pathogenicity studies highlighted the potential role of these bacteria in disease development, especially under environmental stress.ConclusionThis study provides valuable insights into the diversity of bacterial species associated with bacterial disease outbreaks in Indian major carp from aquaculture systems in Tripura. It serves as the first comprehensive investigation of its kind, contributing to our understanding of bacterial infections in Indian major carp.

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Multiple enzymatic profiles of Vibrio parahaemolyticus strains isolated from oysters

Cited by 16 publications

References 13 publications

Evaluating the Anti-Bacterial Activity of Mycelium Extract From Different Wood-Decay-Fungus Species Collected in Southern Vietnam

Evaluating the Anti-Bacterial Activity of Mycelium Extract From Different Wood-Decay-Fungus Species Collected in Southern Vietnam

Vibrio spp. from Yesso scallop (Patinopecten yessoensis) demonstrating virulence properties and antimicrobial resistance

Identification and analysis of pathogenic bacteria causing outbreaks in Indian major carp aquaculture of Tripura

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