Multiple endpoints of polylactic acid biomicroplastic toxicity in adult zebrafish (Danio rerio)

Chagas, Thales Quintão; Freitas, Ítalo Nascimento; Montalvão, Mateus Flores; Nóbrega, Rafael Henrique; Machado, M. R. F.; Charlie-Silva, Ives; Araújo, Amanda Pereira da Costa; Guimarães, Abraão Tiago Batista; Alvarez, Tenilce Gabriela da Silva; Malafaia, Guilherme

doi:10.1016/j.chemosphere.2021.130279

Cited by 60 publications

(10 citation statements)

References 68 publications

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“…Ecotoxicity data for the target micro-bioplastics are limited and have received little attention to date compared to conventional plastics. ,,,− However, available studies indicate that micro-bioplastics have the potential to adversely affect exposed organisms, including freshwater organisms (Chlamydomonas reinhardtii , Anabaena sp., and Daphnia magna) (at concentrations of 25–100 mg/L), zebrafish larvae (Danio rerio) (3 and 9 mg/L), adult zebrafish (D. rerio) (2.5 and 5 mg/L), dragonfly larvae (Aphylla williamsoni) (6 mg/L), D. magna (10–500 mg/L), and marine algae (Chlorella vulgaris) (100 and 1000 mg/L), but at concentrations (high mg/L) above those detected in the environmental samples in this study.…”

Section: Resultsmentioning

confidence: 99%

Identification and Quantification of Micro-Bioplastics in Environmental Samples by Pyrolysis–Gas Chromatography–Mass Spectrometry

Okoffo

Chan

Rauert

et al. 2022

Environ. Sci. Technol.

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Bioplastics are materials that are biobased and/or biodegradable, but not necessarily both. Concerns about environmental plastic pollution are constantly growing with increasing demand for substituting fossil-based plastics with those made using renewable resource feedstocks. For many conventional bioplastics to completely decompose/degrade, they require specific environmental conditions that are rarely met in natural ecosystems, leading to rapid formation of micro-bioplastics. As global bioplastic production and consumption/use continue to increase, there is growing concern regarding the potential for environmental pollution from micro-bioplastics. However, the actual extent of their environmental occurrence and potential impacts remains unclear, and there is insufficient mass concentration-based quantitative data due to the lack of quantitative analytical methods. This study developed and validated an analytical method coupling pressurized liquid extraction and pyrolysis–gas chromatography–mass spectrometry combined with thermochemolysis to simultaneously identify and quantify five targeted micro-bioplastics (i.e., polylactic acid (PLA), polyhydroxyalkanoate, polybutylene succinate, polycaprolactone, and polybutylene adipate terephthalate (PBAT)) in environmental samples on a polymer-specific mass-based concentration. The recovery of spiked micro-bioplastics in environmental samples (biosolids) ranged from 74 to 116%. The limits of quantification for the target micro-bioplastics were between 0.02 and 0.05 mg/g. PLA and PBAT were commonly detected in wastewater, biosolids, and sediment samples at concentrations between 0.07 and 0.18 mg/g. The presented analytical method enables the accurate identification, quantification, and monitoring of micro-bioplastics in environmental samples. This study quantified five micro-bioplastic types in complex environmental samples for the first time, filling in gaps in our knowledge about bioplastic pollution and providing a useful methodology and important reference data for future research.

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Section: Resultsmentioning

confidence: 99%

Identification and Quantification of Micro-Bioplastics in Environmental Samples by Pyrolysis–Gas Chromatography–Mass Spectrometry

Okoffo

Chan

Rauert

et al. 2022

Environ. Sci. Technol.

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“…Moreover, a redox imbalance caused by the decreased activity of superoxide dismutase and total thiol levels was reported as well. When studying Danio rerio adult specimens, Chagas et al [83] also demonstrated the anticholinergic activity of PLA bio-MPs on dragonfly larvae by way of decreased AChE activity, the consequences of which included changes in various neurophysiological functions.…”

Section: Freshwater Ecosystemsmentioning

confidence: 99%

“…Moreover, the high toxicity of degraded PLA bio-MPs was evidenced by the oxidative stress and mitochondrial structural damage, as well as depolarisation, fission inhibition, and apoptosis. Once the fact that PLA bio-MPs cause behavioural changes and biochemical dysfunctions on Danio rerio larvae was demonstrated, Chagas et al [83] studied the consequences of exposing adult fish to the biopolymer. PLA bio-MPs at concentrations of 2.5 and 5.0 mg l −1 accumulated in the liver, brain, gills, and carcass of those fishes.…”

Section: Freshwater Ecosystemsmentioning

confidence: 99%

Biodegradable plastics in aquatic ecosystems: latest findings, research gaps, and recommendations

Ribba

Lopretti

Oca-Vásquez³

et al. 2022

Environ. Res. Lett.

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Plastic accumulation's negative impact on aquatic ecosystems is a known and undeniable problem. Much of the scientific community's efforts are focused on the effects of the most common commodity plastics, but the consequences of the so-called biodegradable plastics in these ecosystems have been little discussed. Although their biodegradable characteristic generates the widespread belief that they are harmless to the environment, it has been proven many years ago that this property cannot be taken lightly. The material´s end-of-life fate is critical to classify it as biodegradable or not. In this context, many plastics classified as biodegradable do not meet the requirements of norms and standards to be considered biodegradable in aquatic ecosystems. Furthermore, during the last five years, the scientific community has shown that they can give rise to the formation of bio-microplastics during their degradation, which can have similar effects to those of conventional microplastics or even worse. This review will detail all recent information regarding how biodegradable plastics can influence aquatic ecosystems, causing adverse health effects in living beings or acting as vectors of chemical pollutants. Besides, the key points that must be addressed in greater depth will be identified, including the need to consider a greater variety of biodegradable plastics and develop systematic methods that allow quantifying and identifying the remains of these pollutants in living species. Another aspect to consider is the dynamics of arrival and mobilization of microplastics in the oceans. It should be studied how small animals fed by filterings, such as red crabs and other zooplankton organisms, move the microplastic through the water column and get into food webs. These particles are mistakenly ingested by the number of species at different trophic levels, where bioaccumulation in tissues must be considered a toxicity factor. Finally, a series of recommendations and future perspectives will be listed.

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“…Some studies also investigated exploration, aversion to novelty, partner preference and social interaction ( Gillera et al, 2020 ; Krentzel et al, 2021 ). The impact of plastic particles on behavior is much less established, with only few studies reporting altered predator-prey interactions and hiding responses, decreased motility or changes to social interactions upon MP exposure of fish ( Sarasamma et al, 2020 ; de Sá et al, 2015 ; Chagas et al, 2021 ; Wen et al, 2018 ; Yin et al, 2018 ; Chen et al, 2020b ; Santos et al, 2021 ) or crustaceans ( Gambardella et al, 2017 ; Tosetto et al, 2016b ; Rehse et al, 2016 ; Suwaki et al, 2020 ; Bai et al, 2021 ). Given the reproductive deficit seen with MPs/NPs and the alterations seen in sex steroid hormone levels (as discussed below in MPs/NPs Alter Nuclear Hormone Signaling and Biotransformation ), an interesting question is whether and to what extent MPs and NPs disturb related complex social behaviors, such as sexual behavior.…”

Section: The Effects Of Microplastics and Nanoplastics On Reproductiv...mentioning

confidence: 99%

Microplastics and Their Impact on Reproduction—Can we Learn From the C. elegans Model?

et al. 2022

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Biologically active environmental pollutants have significant impact on ecosystems, wildlife, and human health. Microplastic (MP) and nanoplastic (NP) particles are pollutants that are present in the terrestrial and aquatic ecosystems at virtually every level of the food chain. Moreover, recently, airborne microplastic particles have been shown to reach and potentially damage respiratory systems. Microplastics and nanoplastics have been shown to cause increased oxidative stress, inflammation, altered metabolism leading to cellular damage, which ultimately affects tissue and organismal homeostasis in numerous animal species and human cells. However, the full impact of these plastic particles on living organisms is not completely understood. The ability of MPs/NPs to carry contaminants, toxic chemicals, pesticides, and bioactive compounds, such as endocrine disrupting chemicals, present an additional risk to animal and human health. This review will discusses the current knowledge on pathways by which microplastic and nanoplastic particles impact reproduction and reproductive behaviors from the level of the whole organism down to plastics-induced cellular defects, while also identifying gaps in current knowledge regarding mechanisms of action. Furthermore, we suggest that the nematode Caenorhabditis elegans provides an advantageous high-throughput model system for determining the effect of plastic particles on animal reproduction, using reproductive behavioral end points and cellular readouts.

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Multiple endpoints of polylactic acid biomicroplastic toxicity in adult zebrafish (Danio rerio)

Cited by 60 publications

References 68 publications

Identification and Quantification of Micro-Bioplastics in Environmental Samples by Pyrolysis–Gas Chromatography–Mass Spectrometry

Identification and Quantification of Micro-Bioplastics in Environmental Samples by Pyrolysis–Gas Chromatography–Mass Spectrometry

Biodegradable plastics in aquatic ecosystems: latest findings, research gaps, and recommendations

Microplastics and Their Impact on Reproduction—Can we Learn From the C. elegans Model?

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