Multiple duplications of the rare ace-1 mutation F290V in Culex pipiens natural populations

Alout, Haoués; Labbé, Pierrick; Berthomieu, Arnaud; Pasteur, Nicole; Weill, Mylène

doi:10.1016/j.ibmb.2009.10.005

Cited by 38 publications

(64 citation statements)

References 36 publications

(38 reference statements)

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“…Ducos is homozygous for the ace-1 D1 allele, a duplicated allele with a susceptible copy and the resistant R copy, in the Slab genetic background (Labbé et al, 2007). HomoVAL is homozygous for the ace-1 V allele (Alout et al, 2009).…”

Section: Strains and Crossing Experimentsmentioning

confidence: 99%

“…AChE1 phenotypes encoded by the ace-1 locus were determined with the témoin-dichlorvos-prpopoxur test on head homogenates of single adults (Alout et al, 2009). This test discriminates between phenotype S (that is, homozygotes for the S allele), phenotype R or V (homozygotes for the G119S or the F290V mutation, respectively) and phenotypes SR or SV that contain at least one S copy and one resistant R or V copy (for example,…”

Section: Bioassays and Insecticide Selectionsmentioning

confidence: 99%

“…Interaction between the T allele and resistant alleles of the ace-1 gene. Two main resistant mutations have been selected at the ace-1 locus in Mediterranean mosquito populations (Alout et al, 2009): the R allele carrying the G119S mutation and the V allele carrying the F290V mutation. When homozygous in a strain, the R allele confers 19-fold and the V allele confers 8.4-fold resistance to chlorpyrifos at LC 50 , respectively (Table 1).…”

Section: Synergistic Interaction Between Resistance Genes H Alout Et Almentioning

confidence: 99%

“…In the mosquito Culex pipiens, various amplified esterases (encoded by the Ester super locus) and two substitutions on the AChE1 (encoded by the ace-1 gene) have been identified; they confer resistance to a large variety of OP and carbamate insecticides. Mutations at the ace-1 gene include the G119S and the F290V substitutions (the R and V alleles, respectively, Weill et al, 2003;Alout et al, 2007), as well as several duplications (D alleles) containing, in tandem, a susceptible copy (S) and a resistant copy (R or V, Labbé et al, 2007;Alout et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes

et al. 2015

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We investigated the genetic determinism of high chlorpyrifos resistance (HCR), a phenotype first described in 1999 in Culex pipiens mosquitoes surviving chlorpyrifos doses ⩾ 1 mg l − 1 and more recently found in field samples from Tunisia, Israel or Indian Ocean islands. Through chlorpyrifos selection, we selected several HCR strains that displayed over 10 000-fold resistance. All strains were homozygous for resistant alleles at two main loci: the ace-1 gene, with the resistant ace-1 R allele expressing the insensitive G119S acetylcholinesterase, and a resistant allele of an unknown gene (named T) linked to the sex and ace-2 genes. We constructed a strain carrying only the T-resistant allele and studied its resistance characteristics. By crossing this strain with strains harboring different alleles at the ace-1 locus, we showed that the resistant ace-1 R and the T alleles act in strong synergy, as they elicited a resistance 100 times higher than expected from a simple multiplicative effect. This effect was specific to chlorpyrifos and parathion and was not affected by synergists. We also examined how HCR was expressed in strains carrying other ace-1-resistant alleles, such as ace-1 V or the duplicated ace-1 D allele, currently spreading worldwide. We identified two major parameters that influenced the level of resistance: the number and the nature of the ace-1-resistant alleles and the number of T alleles. Our data fit a model that predicts that the T allele acts by decreasing chlorpyrifos concentration in the compartment targeted in insects.

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Section: Strains and Crossing Experimentsmentioning

confidence: 99%

Section: Bioassays and Insecticide Selectionsmentioning

confidence: 99%

Section: Synergistic Interaction Between Resistance Genes H Alout Et Almentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes

et al. 2015

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“…Two collections, Ste Suzanne and St Leu, were allowed to breed in the laboratory for establishing two strains (Suz and Leu) which were maintained during the course of this study. Reference strains included Slab susceptible line (Georghiou et al, 1966), SR and Homoval lines which are homozygous for the G119S or F290V resistant AChE1 alleles, respectively (Alout et al, 2009;Berticat et al, 2002), and various lines homozygous for different over-produced esterases [SA1, SA2, SA4, Berticat et al, 2002]. Ae.…”

Section: Mosquito Samples and Strainsmentioning

confidence: 99%

Insecticide resistance in Culex pipiens quinquefasciatus and Aedes albopictus mosquitoes from La Réunion Island

Tantely

Tortosa

Alout

et al. 2010

Insect Biochemistry and Molecular Biology

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Susceptibility status of Culex quinquefasciatus (Diptera: Culicidae) populations to the chemical insecticide temephos in Pernambuco, Brazil

et al. 2013

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Multiple duplications of the rare ace-1 mutation F290V in Culex pipiens natural populations

Cited by 38 publications

References 36 publications

High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes

High chlorpyrifos resistance in Culex pipiens mosquitoes: strong synergy between resistance genes

Insecticide resistance in Culex pipiens quinquefasciatus and Aedes albopictus mosquitoes from La Réunion Island

Susceptibility status of Culex quinquefasciatus (Diptera: Culicidae) populations to the chemical insecticide temephos in Pernambuco, Brazil

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