Multiple drug resistance and strength of attachment to surfaces in Pseudomonas aeruginosa isolates

Abstract

Aims: To investigate the presence of a relationship between the strength of attachment of Pseudomonas aeruginosa to stainless steel surfaces and their observed multiple drug resistance. Methods and Results: Multiple drug resistance of clinical and environmental isolates of Ps. aeruginosa was evaluated using disc diffusion method. The blot succession technique was used to quantify the strength of attachment of Ps. aeruginosa isolates. Different multiple drug–resistant Ps. aeruginosa isolates exhibited variable attachment strength. Although the highest multiple drug–resistant clinical isolate was shown to have the least attachment strength among clinical isolates, a weak correlation was found between attachment strength and multiple resistance among our investigated Ps. aeruginosa isolates. Conclusions: There is a weak correlation between multiple drug resistance and strength of attachment to stainless steel surfaces. Significance and Impact of the Study: Even low‐resistant Ps. aeruginosa could have the potential of attaching firmly to surfaces and forming biofilm.

Investigating possible association between multidrug resistance and isolate origin with some virulence factors ofEscherichia colistrains isolated from infant faeces and fresh green vegetables

“…In addition, biofilm formation was comparable among the four groups of isolates studied (R-Plant, S-Plant, R-Faecal and S-Faecal). These results are in line with those previously reported by Haddadin et al (2010a). However, in that study they could not detect a correlation between MDR or non-MDR Pseudomonas aeruginosa and the strength of attachment to stainless steel surfaces.…”

“…These results are in line with those previously reported by Haddadin et al . (). However, in that study they could not detect a correlation between MDR or non‐MDR Pseudomonas aeruginosa and the strength of attachment to stainless steel surfaces.…”

Quaternary Ammonium Compounds (QACs) induced inactivation of Pseudomonas spp.: Effect of material surface

“…This increase in resistance to QACs can be demonstrated by the significant increase in the MIC value (Minimum inhibitory concentration) in adapted cells (Haddadin et al, 2010;To et al, 2002). Degradation of these compounds and, changes in fatty acid composition and expression of efflux pump genes are some adaptive mechanisms to QACs showed by Pseudomonas spp.…”

“…In the present study, by supplementing the TSB medium with the QACs, the lag phase has been considerably extended to 24 hours and a lower OD was observed (0.68) after 48 hours. According toLucchesi et al (2010) and(Haddadin et al, 2010), this low increase in cell population in the presence of QACs could be related to the accumulation of degradation metabolites of QACs which prevented further cell proliferation.…”

“…The lag phase in Pseudomonas fragi and Pseudomonas fluorescens was longer compared toPseudomonas putida and entered the stationary phase after 24 h with a max OD = 2.6.Figure 2clearly demonstrates that the growth of all adapted Pseudomonas strains was significantly slowed down in the presence of QACs. According to other researchers(Haddadin et al, 2010), one of the adaptation mechanisms of Pseudomonas strains to QACs is attributed to the induction of stress response and the slow growth of bacteria. In the present study, by supplementing the TSB medium with the QACs, the lag phase has been considerably extended to 24 hours and a lower OD was observed (0.68) after 48 hours.…”