Multiple crystallization as a potential strategy for efficient recovery of succinic acid following fermentation with immobilized cells

Luthfi, Abdullah Amru Indera; Tan, Jian Ping; Isa, Nur Fatin Ajeera Mohd; Bukhari, Nurul Adela; Shah, Siti Syazwani Mohd; Mahmod, Safa Senan; Jahim, Jamaliah Md

doi:10.1007/s00449-020-02311-x

Cited by 14 publications

(8 citation statements)

References 61 publications

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“…The lesson learned was to include low-pH fermentation and an efficient DSP to make SA market ready 36,37 . Furthermore, several studies have attempted to recover SA from fermentation broth via direct acidification and crystallization 4,35,38,39,40,41 . In these studies, direct crystallization was highlighted as an effective technique to recover SA from fermentation broth.…”

Section: Discussionmentioning

confidence: 99%

An End-to-end Pipeline for Succinic Acid Production at an Industrially Relevant Scale usingIssatchenkia orientalis

Tran

Mishra

Bhagwat

et al. 2023

Preprint

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As one of the top value-added chemicals, succinic acid has been the focus of numerous metabolic engineering campaigns since the 1990s. However, microbial production of succinic acid at an industrially relevant scale has been hindered by high downstream processing costs arising from neutral pH fermentation. Here we describe the metabolic engineering of Issatchenkia orientalis, a non-conventional yeast with superior tolerance to highly acidic conditions, for cost-effective succinic acid production. Through deletion of byproduct pathways, transport engineering, and expanding the substrate scope, the resulting strains could produce succinic acid at the highest titers in sugar-based media at low pH (pH 3) in fed-batch fermentations using bench-top reactors, i.e. 109.5 g/L in minimal medium and 104.6 g/L in sugarcane juice medium. We further performed batch fermentation in a pilot-scale fermenter with a scaling factor of 300-fold, achieving 63.1 g/L of succinic acid using sugarcane juice medium. A downstream processing comprising of two-stage vacuum distillation and crystallization enabled direct recovery of succinic acid, without further acidification of fermentation broth, with an overall yield of 64.0%. Finally, we simulated an end-to-end low-pH succinic acid production pipeline, and techno-economic analysis and life cycle assessment indicate our process is financially viable and can reduce life cycle greenhouse gas emissions by 34-90% relative to fossil-based production processes. We expect I. orientalis can serve as a general industrial platform for the production of a wide variety of organic acids.

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Section: Discussionmentioning

confidence: 99%

An End-to-end Pipeline for Succinic Acid Production at an Industrially Relevant Scale usingIssatchenkia orientalis

Tran

Mishra

Bhagwat

et al. 2023

Preprint

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“…The demerit can be overcome by selecting a good support material for cell adhesion as a key success for the immobilization process. Coconut shell-activated carbon (CSAC) is the potential support material with the criteria as inert, costeffective, has a greater mechanical strength, good wear resistance, higher surface area and porosity due to the internal granular structure than any other activated carbons of a similar grade [25]. The determining factor for an excellent support material is to have high speci c surface area result in high productivity of lactic acid [26].…”

Section: Introductionmentioning

confidence: 99%

Biotechnological enhancement of lactic acid conversion from palm kernel cake by immobilized Actinobacillus succinogenes 130Z

Rahim

Luthfi

Bukhari

et al. 2023

Preprint

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The aim of this study was to establish an improved pretreatment and fermentation method i.e. immobilized cells for high recovery of fermentable sugars from PKC and its effects on fermentability performance by Actinobacillus succinogenes 130Z in the conversion of lactic acid. The effects of oxalic acid concentrations (1–6% w/v) and residence times (1–5 h) on the sugar recovery were initially investigated and it was found that the highest mannose concentration was 25.1 g/L at the optimum hydrolysis conditions of 4 h and 3% (w/v) oxalic acid. The subsequent enzymatic saccharification of the pretreated PKC afforded the highest enzymatic digestibility with the recovered sugars amounting to 25.18 g/L and 9.14 g/L of mannose and glucose, respectively. Subsequently, the fermentability performance of PKC hydrolysate was evaluated and compared in terms of cultivation phases (i.e. mono and dual-phases), carbonate loadings (i.e. magnesium and sodium carbonates), and types of sugars (i.e. glucose and mannose). The highest titer of 19.4 g/L lactic acid was obtained from the fermentation involving A. succinogenes 130Z in dual-phase cultivation supplemented with 30 g/L of magnesium carbonate. Lactic acid production was further enhanced by using immobilized cells with coconut shell-activated carbon (CSAC) of different sizes (A, B, C, and D) in the repeated batch cultivation of dual-phase fermentation producing 31.64 g/L of lactic acid. This work sheds light on the possibilities to enhance the utilization of PKC for lactic acid production via immobilized A. succinogenes 130Z.

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“…The demerit can be overcome by selecting a good support material for cell adhesion as a key success for the immobilization process. Coconut shell-activated carbon (CSAC) is the potential support material with the criteria as inert, cost-effective, has a greater mechanical strength, good wear resistance, higher surface area and porosity due to the internal granular structure than any other activated carbons of a similar grade 26 . The determining factor for an excellent support material is to have a high specific surface area, resulting in high productivity of lactic acid 26 .…”

Section: Introductionmentioning

confidence: 99%

“…Coconut shell-activated carbon (CSAC) is the potential support material with the criteria as inert, cost-effective, has a greater mechanical strength, good wear resistance, higher surface area and porosity due to the internal granular structure than any other activated carbons of a similar grade 26 . The determining factor for an excellent support material is to have a high specific surface area, resulting in high productivity of lactic acid 26 . Therefore, the different sizes of specific surface area of CSAC were compared to develop an industrially feasible fermentation using PKC hydrolysate.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

Rahim

Luthfi

Bukhari

et al. 2023

Sci Rep

Self Cite

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The aim of this study was to establish an improved pretreatment and fermentation method i.e. immobilized cells for high recovery of fermentable sugars from palm kernel cake (PKC) and its effects on fermentability performance by Actinobacillus succinogenes 130Z in the conversion of the fermentable sugar to lactic acid. The effects of oxalic acid concentrations (1–6% w/v) and residence times (1–5 h) on the sugar recovery were initially investigated and it was found that the highest mannose concentration was 25.1 g/L at the optimum hydrolysis conditions of 4 h and 3% (w/v) oxalic acid. The subsequent enzymatic saccharification of the pretreated PKC afforded the highest enzymatic digestibility with the recovered sugars amounting to 25.18 g/L and 9.14 g/L of mannose and glucose, respectively. Subsequently, the fermentability performance of PKC hydrolysate was evaluated and compared in terms of cultivation phases (i.e. mono and dual-phases), carbonate loadings (i.e. magnesium and sodium carbonates), and types of sugars (i.e. glucose and mannose). The highest titer of 19.4 g/L lactic acid was obtained from the fermentation involving A. succinogenes 130Z in dual-phase cultivation supplemented with 30 g/L of magnesium carbonate. Lactic acid production was further enhanced by using immobilized cells with coconut shell-activated carbon (CSAC) of different sizes (A, B, C, and D) in the repeated batch cultivation of dual-phase fermentation producing 31.64 g/L of lactic acid. This work sheds light on the possibilities to enhance the utilization of PKC for lactic acid production via immobilized A. succinogenes 130Z.

show abstract

Multiple crystallization as a potential strategy for efficient recovery of succinic acid following fermentation with immobilized cells

Cited by 14 publications

References 61 publications

An End-to-end Pipeline for Succinic Acid Production at an Industrially Relevant Scale usingIssatchenkia orientalis

An End-to-end Pipeline for Succinic Acid Production at an Industrially Relevant Scale usingIssatchenkia orientalis

Biotechnological enhancement of lactic acid conversion from palm kernel cake by immobilized Actinobacillus succinogenes 130Z

Biotechnological enhancement of lactic acid conversion from pretreated palm kernel cake hydrolysate by Actinobacillus succinogenes 130Z

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