Multiple crosstalk between TOR and the cell integrity MAPK signaling pathway in fission yeast

Madrid, Marisa; Vázquez-Marín, Beatriz; Franco, Alejandro; Soto, Teresa; Vicente-Soler, Jero; Gacto, Mariano; Cansado, José

doi:10.1038/srep37515

Cited by 36 publications

(33 citation statements)

References 55 publications

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“…Our results support recently findings related to a cross‐regulatory relationship between CaMK and TOR pathways . And, significantly reduced transcription of the ght5 gene by increased iron stress has been thought that Ght5 protein might play a central role in complicated cross‐regulatory relationship.…”

Section: Discussionsupporting

confidence: 92%

Iron regulates hexose transporters in Schizosaccharomyces pombe

et al. 2019

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This study focuses on the effect of iron on hexose transporters which perform glucose uptake. For this aim, we investigated the role of iron in glucose utilization and expression of hexose transporters in Schizosaccharomyces pombe. We applied different iron concentrations (1, 2, 5, 10 mM) to the cells grown up to mid-logarithmic phase. According to analysis of cell viability and morphology, we determined 2 mM and 5 mM as non-toxic and toxic doses, respectively. Besides, glucose consumption efficiency increased (1.5-fold) in the cells which were exposed to these iron concentrations. qRT-PCR analysis of hexose transporter genes showed that the expression of ght2 and ght8 genes were downregulated under both non-toxic and toxic iron conditions, but that of ght5 gene was significantly decreased only by toxic iron dose. In conclusion, it was suggested for the first time in this study that the Ght5 protein, as being high affinity hexose transporter, might play a role in sensing and signaling of iron stress. K E Y W O R D Shexose transporters, iron stress, Schizosaccharomyces pombe

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Section: Discussionsupporting

confidence: 92%

Iron regulates hexose transporters in Schizosaccharomyces pombe

et al. 2019

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“…Whereas the reduction of the TORC1 activity leads to ectopic entry into sexual differentiation, the disruption of the psk1 gene shows little effect on the growth mode [ 20 ]. It has been recently suggested that Psk1 cooperates with TORC2-target Gad8 to adapt to cell integrity stress and glucose starvation, although the precise mechanism remains unclear [ 25 ].…”

Section: Conserved Phosphorylation Targets Of Torc1mentioning

confidence: 99%

TORC1-Dependent Phosphorylation Targets in Fission Yeast

et al. 2017

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Target of rapamycin (TOR) kinase controls cell metabolism and growth in response to environmental cues such as nutrients, growth factors, and stress. TOR kinase is widely conserved across eukaryotes. As in other organisms, the fission yeast Schizosaccharomyces pombe has two types of TOR complex, namely TOR complex 1 (TORC1) and TORC2. It is interesting that the two TOR complexes in S. pombe have opposite roles in sexual differentiation, which is induced by nutrient starvation. TORC1, which contains Tor2 as a catalytic subunit, promotes vegetative growth and represses sexual differentiation in nutrient-rich conditions, while TORC2 is required for the initiation of sexual differentiation. Multiple targets of TORC1 have been identified. Some of these, such as S6 kinase and an autophagy regulator Atg13, are known targets in other organisms. In addition, there is a novel group of TORC1 targets involved in the regulation of sexual differentiation. Here, we review recent findings on phosphorylation targets of TORC1 in S. pombe. Furthermore, we briefly report a novel S. pombe target of TORC1.

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“…Multiple cross-regulatory interactions were identified between the TOR and the Mitogen Activated Protein Kinase (MAPK) signalling pathways in the fission yeast and these cross-talk were proposed as mechanisms to elicit adaptive responses to extra- and intracellular conditions by regulating essential cellular functions [ 31 ]. MAPK signalling pathways, particularly those of mating and filamentous growth, were shown to be responsive specifically to intermediary levels of oxygen availability in the baker’s yeast.…”

Section: Introductionmentioning

confidence: 99%

Saccharomyces cerevisiae adapted to grow in the presence of low-dose rapamycin exhibit altered amino acid metabolism

et al. 2018

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BackgroundRapamycin is a potent inhibitor of the highly conserved TOR kinase, the nutrient-sensitive controller of growth and aging. It has been utilised as a chemotherapeutic agent due to its anti-proliferative properties and as an immunosuppressive drug, and is also known to extend lifespan in a range of eukaryotes from yeast to mammals. However, the mechanisms through which eukaryotic cells adapt to sustained exposure to rapamycin have not yet been thoroughly investigated.MethodsHere, S. cerevisiae response to long-term rapamycin exposure was investigated by identifying the physiological, transcriptomic and metabolic differences observed for yeast populations inoculated into low-dose rapamycin-containing environment. The effect of oxygen availability and acidity of extracellular environment on this response was further deliberated by controlling or monitoring the dissolved oxygen level and pH of the culture.ResultsYeast populations grown in the presence of rapamycin reached higher cell densities complemented by an increase in their chronological lifespan, and these physiological adaptations were associated with a rewiring of the amino acid metabolism, particularly that of arginine. The ability to synthesise amino acids emerges as the key factor leading to the major mechanistic differences between mammalian and microbial TOR signalling pathways in relation to nutrient recognition.ConclusionOxygen levels and extracellular acidity of the culture were observed to conjointly affect yeast populations, virtually acting as coupled physiological effectors; cells were best adapted when maximal oxygenation of the culture was maintained in slightly acidic pH, any deviation necessitated more extensive readjustment to additional stress factors.Electronic supplementary materialThe online version of this article (10.1186/s12964-018-0298-y) contains supplementary material, which is available to authorized users.

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Multiple crosstalk between TOR and the cell integrity MAPK signaling pathway in fission yeast

Cited by 36 publications

References 55 publications

Iron regulates hexose transporters in Schizosaccharomyces pombe

Iron regulates hexose transporters in Schizosaccharomyces pombe

TORC1-Dependent Phosphorylation Targets in Fission Yeast

Saccharomyces cerevisiae adapted to grow in the presence of low-dose rapamycin exhibit altered amino acid metabolism

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