Multiple Copies of β-Lactoglobulin Promoter Do Not Function as LCR

James, R. M.; Neil, C.; Webster, John; Roos, Stefan; Clark, Anthony; Whitelaw, Bruce

doi:10.1006/bbrc.2000.2766

Cited by 5 publications

(2 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An additional, less characterized site has been located at −2500 bp [10,12]. Although hypersensitive sites usually indicate the presence of cis ‐regulatory elements, only the 400‐bp proximal region of the BLG promoter has been studied in detail [12–14].…”

Section: Introductionmentioning

confidence: 99%

BLG‐e1 – A novel regulatory element in the distal region of the β‐lactoglobulin gene promoter

2005

View full text Add to dashboard Cite

b-Lactoglobulin (BLG) is a major ruminant milk protein. A regulatory element, termed BLG-e1, was defined in the distal region of the ovine BLG gene promoter. This 299-bp element lacks the established cis-regulatory sequences that affect milk-protein gene expression. Nevertheless, it alters the binding of downstream BLG sequences to histone H4 and the sensitivity of the histone-DNA complexes to trichostatin A treatment. In mammary cells cultured under favorable lactogenic conditions, BLG-e1 acts as a potent, position-independent silencer of BLG/luciferase expression, and similarly affects the promoter activity of the mouse whey acidic protein gene. Intragenic sequences upstream of BLG exon 2 reverse the silencing effect of BLG-e1 in vitro and in transgenic mice.

show abstract

Section: Introductionmentioning

confidence: 99%

BLG‐e1 – A novel regulatory element in the distal region of the β‐lactoglobulin gene promoter

2005

View full text Add to dashboard Cite

show abstract

“…In this way, expression of genes normally active in the liver are directed to the mammary gland and hence into the milk. Attempts to improve transgene expression have largely focussed on enhancing transcription frequency within a population of founder animals , increasing transcription level within a line (James et al, 2000;Pantano et al, 2003) or through the use of large genomic fragments that are considered to contain the necessary regulatory elements (Giraldo & Montoliu, 2001).…”

Section: Introductionmentioning

confidence: 99%

On the Use of Post-Transcriptional Processing Elements in Transgenes

et al. 2004

View full text Add to dashboard Cite

show abstract

In vitro and in vivo effects of a multimerized αs1‐casein enhancer on whey acidic protein gene promoter activity

Pantano

Rival‐Gervier²,

Prince³

et al. 2003

Molecular Reproduction Devel

View full text Add to dashboard Cite

Experimental data obtained in previous works have led to postulate that enhancers increase the frequency of action of a linked promoter in a given cell and may have some insulating effects. The multimerized rabbit alpha s1-casein gene enhancer, the 6i multimer, was added upstream of the rabbit whey acidic protein gene (WAP) promoter (-6,300; +28 bp) fused to the firefly luciferase (luc) gene (6i WAP-luc construct). The 6i multimer increased reporter gene expression in mouse mammary HC11 cells. In transgenic mice, a very weak but significant increase was also observed. More noticeable, no silent lines were found when the 6i multimer was associated to the WAP-luc construct. This reflects the fact that the 6i multimer tends to prevent the silencing of the WAP-luc construct. After addition of the 5'HS4 insulator region from the chicken beta-globin locus upstream of the 6i multimer, similar luciferase levels were measured in 6i WAP-luc and 5'HS4 WAP-luc transgenic mice. Our present data and previous ones, which show that the 6i multimer has no insulating activity on a TK gene promoter construct indicate that the insulating activity of the 6i multimer is construct-dependent and not amplified by the 5'HS4 insulator.

show abstract

Multiple Copies of β-Lactoglobulin Promoter Do Not Function as LCR

Cited by 5 publications

References 31 publications

BLG‐e1 – A novel regulatory element in the distal region of the β‐lactoglobulin gene promoter

BLG‐e1 – A novel regulatory element in the distal region of the β‐lactoglobulin gene promoter

On the Use of Post-Transcriptional Processing Elements in Transgenes

In vitro and in vivo effects of a multimerized αs1‐casein enhancer on whey acidic protein gene promoter activity

Contact Info

Product

Resources

About