Multiple Controls over the Efficiency of Translation of the mRNAs Encoding Transition Proteins, Protamines, and the Mitochondrial Capsule Selenoprotein in Late Spermatids in Mice

Kleene, Kenneth C.

doi:10.1006/dbio.1993.1277

Cited by 63 publications

(72 citation statements)

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“…These results suggest that the chromatoid body of male germ cells and P-bodies in somatic cells are functionally related, both acting as a site for mRNA decay and mRNA translational repression by the miRNA pathway. Thus, our findings provide an attractive interpretation of the phenomenon of translational repression that occurs postmeiotically in spermatids (42,43).…”

Section: Discussionmentioning

confidence: 69%

The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway components

Kotaja

Bhattacharyya

Jaśkiewicz

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

318

319

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The chromatoid body is a perinuclear, cytoplasmic cloud-like structure in male germ cells whose function has remained elusive. Here we show that the chromatoid body is related to the RNA-processing body of somatic cells. Dicer and components of microRNP complexes (including Ago proteins and microRNAs) are highly concentrated in chromatoid bodies. Furthermore, we show that Dicer interacts with a germ cell-specific chromatoid body component, the RNA helicase MVH (mouse VASA homolog). Thus, chromatoid bodies seem to operate as intracellular nerve centers of the microRNA pathway. Our findings underscore the importance of posttranscriptional gene regulation and of the microRNA pathway in the control of postmeiotic male germ cell differentiation.Argonaute ͉ microRNA ͉ spermatogenesis ͉ RNA processing

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Section: Discussionmentioning

confidence: 69%

The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway components

Kotaja

Bhattacharyya

Jaśkiewicz

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

318

319

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“…The reaction was stopped by adding EGTA to a final concentration of 25 mM prior to layering on the sucrose gradient, which was supplemented with EGTA as well. (43,45). Briefly, seminiferous tubules from 7-day postpartum (dpp) mili ϩ/Ϫ or mili Ϫ/Ϫ testes were dissected in RPMI 1640 medium supplemented with 1 mM pyruvic acid and 6 mM lactic acid and then transferred to RPMI medium minus methionine.…”

Section: Methodsmentioning

confidence: 99%

“…S1 and S2). Hence, by isolating seminiferous tubules, we eliminated extratubular somatic cells, thus allowing our measurements to reflect largely the status of protein synthesis in germ line stem cells yet under a physiologically normal condition that could not be achieved if we used isolated germ line stem cells (43,45).…”

Section: Many Poly(a)mentioning

confidence: 99%

MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation

Unhavaithaya

Hao

Beyret

et al. 2009

Journal of Biological Chemistry

195

210

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The Argonaute/PIWI protein family consists of Argonaute and PIWI subfamilies. Argonautes function in RNA interference and micro-RNA pathways; whereas PIWIs bind to PIWIinteracting RNAs and regulate germ line development, stem cell maintenance, epigenetic regulation, and transposition. However, the role of PIWIs in mammalian stem cells has not been demonstrated, and molecular mechanisms mediated by PIWIs remain elusive. Here we show that MILI, a murine PIWI protein, is expressed in the cytoplasm of testicular germ line stem cells, spermatogonia, and early spermatocytes, where it is enriched in chromatoid bodies. MILI is essential for the self-renewing division and differentiation of germ line stem cells but does not affect initial establishment of the germ line stem cell population at 7 days postpartum. Furthermore, MILI forms a stable RNAindependent complex with eIF3a and associates with the eIF4E-and eIF4G-containing m 7 G cap-binding complex. In isolated 7 days postpartum seminiferous tubules containing mostly germ line stem cells, the mili mutation has no effect on the cellular mRNA level yet significantly reduces the rate of protein synthesis. These observations indicate that MILI may positively regulate translation and that such regulation is required for germ line stem cell self-renewal.

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“…For example, some mRNAs required for spermiogenesis are stored for several days in chromatoid bodies, until exiting and entering translation at appropriate developmental timepoints. 121,122 Similarly, a subtype of germ granule referred to as "storage bodies" in the C. elegans gonad rely on the presence of CGH-1 (homolog of the P-body factor Dhh1/RCK) for stabilization of several maternal mRNAs. 123 Stress granules and P-bodies may also harbor stable mRNAs, given that they contain mRNA stabilizing proteins such as HuR and Pab1.…”

Section: Role Of Mrnp Granules In Mrna Stabilitymentioning

confidence: 99%

mRNP granules

2014

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Multiple Controls over the Efficiency of Translation of the mRNAs Encoding Transition Proteins, Protamines, and the Mitochondrial Capsule Selenoprotein in Late Spermatids in Mice

Cited by 63 publications

References 0 publications

The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway components

The chromatoid body of male germ cells: Similarity with processing bodies and presence of Dicer and microRNA pathway components

MILI, a PIWI-interacting RNA-binding Protein, Is Required for Germ Line Stem Cell Self-renewal and Appears to Positively Regulate Translation

mRNP granules

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