Multiple clustered genes of the haemoglobin VIIB subfamily of Chironomus thummi thummi (Diptera)

Trewitt, Patrick M.; Saffarini, Daâd A.; Bergtrom, Gerald

doi:10.1016/0378-1119(88)90381-2

Cited by 23 publications

(24 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The many paralogous genes of the C. thummi Gb I, Gb IA, Gb III/IV, Gb VI, and Gb VIII3 families are intronless (Antoine and Niessing 1984;Antoine et al 1987;Trewitt et al 1987Trewitt et al , 1988Hankeln et al 1988Hankeln et al , 1991Rozynek et al 1989Rozynek et al , 1991Kao and Bergtrom, unpublished data). Thus, the presence of an intron in two members of the C. thummi globin II family was completely unexpected, especially in light of the absence of the intron in their respective C. tentans homologues.…”

Section: Discussionmentioning

confidence: 99%

“…Intron loss is thought to be widespread in insects, perhaps the result of selective pressure to maintain smaller genome size (Hawkins 1988). Thus, in contrast to the conserved intron/exon organization of the globin genes of other organisms (vertebrates, invertebrates, plants), all of the nearly 30 known globin genes of the genus Chironomus are intronless (Antoine and Niessing 1984;Antoine et al 1987;Trewitt et al 1987Trewitt et al , 1988Hankeln et al 1988Hankeln et al , 1991Rozynek et al 1989Rozynek et al , 1991. The presumption has been that the ancestral chironomid globin gene lost its introns soon after the insect and vertebrate lineage separation i>600 million years ago (Lewin 1984).…”

Section: Introductionmentioning

confidence: 99%

“…Subsequent to the putative correction, more differences appear to have accumulated in the intronic DNA than in the flanking exonic DNA within the 123-nt region. We note that partial gene corrections, albeit of somewhat larger regions, have been previously implicated in the evolution of the C. thummi globin III/IV and globin VII[3 subfamilies (Trewitt et al 1988). …”

Section: Sequence Similarity Of the Gb I1t~ And Gb IX Intronsmentioning

confidence: 99%

See 2 more Smart Citations

Intron-containing globin genes in the insect Chironomus thummi

1994

Self Cite

View full text Add to dashboard Cite

All previously reported chironomid globin genes are intronless, suggesting that the ancestral chironomid globin gene was also intronless. In this study, the coding regions of the closely linked Chironomus thummi globin (Gbs) II beta and IX genes are shown to be interrupted by noncoding DNA bounded by a 5'-GT and a 3'-AG. Both genes have appropriately placed transcription and translation signals. Polymerase chain reactions on genomic DNA with oligonucleotides flanking and within the putative Gb II beta intron generated products the size predicted for a gene with a 64-nucleotide intron, and sequencing of a cloned PCR fragment also revealed the intron. A partial-length Gb II beta cDNA sequence exactly matches that of the Gb II beta coding regions. We conclude that the intron-containing chironomid globin genes are functional. Regions of the Gb II beta and IX genes spanning the introns are more similar (86%) than the exons themselves (72% similarity), possibly due to partial gene correction. Surprisingly, Gb II beta and IX gene homologues in C. tentans are intronless. If the common ancestor of chironomid globin genes was not intronless, introns were lost in at least three C. thummi globin-gene lineages, and more recently by Gb II beta and Gb IX genes in C. tentans. If, as previously believed, the ancestral chironomid globin gene was intronless, the ancestral chironomid globin gene was intronless, then an intron was recently acquired in only one C. thummi globin sublineage. These alternatives are discussed.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Sequence Similarity Of the Gb I1t~ And Gb IX Intronsmentioning

confidence: 99%

See 1 more Smart Citation

Intron-containing globin genes in the insect Chironomus thummi

1994

Self Cite

View full text Add to dashboard Cite

show abstract

“…For S I nuclease protection experiments, uniformly labeled single-stranded probes were synthesized from M 13 genomic subclone templates as described previously (Trewitt et al 1988). After digestion with the appropriate multicloning region restriction enzyme, probes were liberated from their template by alkaline gel electrophoresis (Sambrook et al 1989).…”

Section: Methodsmentioning

confidence: 99%

The boll weevil vitellogenin gene: Nucleotide sequence, structure, and evolutionary relationship to nematode and vertebrate vitellogenin genes

Trewitt

Heilmann²,

Degrugillier³

et al. 1992

J Mol Evol

Self Cite

View full text Add to dashboard Cite

Boll weevil (Anthonomus grandis) eggs contain two yolk proteins, YP47 and YP160. Using anti-YP160 antiserum as probe, a partial-length complementary DNA (cDNA) was isolated from a lambda gt11 adult female cDNA library. A second partial-length cDNA was isolated from a lambda gt10 adult female cDNA library by differential screening with male vs. female cDNAs. Northern blot analysis showed that each cloned cDNA hybridized to a 6-kb female-specific transcript. These cDNAs were used to probe a genomic library, and two overlapping genomic clones were obtained that span the boll weevil vitellogenin gene. The entire transcription unit was sequenced, and introns were mapped by a combination of primer extension experiments, S1 nuclease protection experiments, and polymerase chain reaction-mediated synthesis of two additional cDNA clones. Based on these data, the vitellogenin mRNA is 5511 nucleotides [plus a poly(A) tail of undetermined length] and specifies a provitellogenin of 1790 amino acids. The deduced protein has a Glu+Gln content of 16.3%, which is a relatively high value that is typical of most vitellogenins. Protein sequence similarities including Cys clusters conserved between boll weevil vitellogenin and Xenopus laevis A2 or Caenorhabditis elegans vit-5 vitellogenins indicated that the boll weevil protein is a member of the ancient nematode-vertebrate vitellogenin family. Moreover, the six introns in the boll weevil vitellogenin gene interrupt the coding region at positions closely or exactly corresponding to a subset of the positions of the 34 vertebrate vitellogenin introns, further supporting the argument for a common evolutionary relationship. This report represents the first complete nucleotide sequence and structural analysis of a nondipteran insect vitellogenin gene.

show abstract

“…Therefore, 7211/7447 may contain core elements, which are present within 7211/7285, as well as essential auxiliary elements for the full promoter activity. Three putative Sp1 elements (boxes 2, 3, and 4) and one HES-1 binding (1) Tsujimoto and Suzuki (1979); (2) Trewitt et al (1988); (3) Rajput et al (1994); (4) Hillarp et al (1993); (5) Leutwiler et al (1986); (6) Sanicola et al (1990); (7) site are present within the nucleotide sequence from 7286 to 7447, where the auxiliary elements are thought to be located. Theoretically, the presence of multiple Sp1 elements can further increase the basal promoter activity.…”

Section: Discussionmentioning

confidence: 99%

Human Dbf4/ASK promoter is activated through the Sp1 and MluI cell-cycle box (MCB) transcription elements

Wan

Lee

2002

Oncogene

View full text Add to dashboard Cite

Dbf4 is the regulatory subunit of Cdc7 kinase, which is essential for entry into and traversing through S phase. The level of Dbf4, which is critical for the activation of Cdc7, is regulated by transcription and protein degradation. To gain a better understanding as to how the transcription of human Dbf4 (HuDbf4) is regulated, we have cloned and characterized its promoter. We found that HuDbf4 core promoter is localized within 7 211 to 7285 of the translation start-codon. This 75 bp DNA segment contains, among others, a putative MluI Cellcycle Box (MCB). A point mutation within the MCB dramatically reduced the promoter activity. This is the first example that an MCB element plays an essential role in the activation of a core promoter in mammalian cells. The auxiliary elements required for the full promoter activity are present within 162-bp upstream from the core promoter (i.e., 7286/7447). A point mutation within the Sp1 element at 7353/7361 resulted in a decrease of promoter activity to the basal level, while the deletion of the putative HES-1 at 7326/7331 dramatically increased the promoter activity. Taken together, our data suggests that the MCB element is essential for the core promoter activation, while the Sp1 positive regulator and the HES-1 repressor coordinately determine the efficiency of the HuDbf4 promoter. We have also found: (i) that the major transcription initiations occur at 7220, 7235 and 7245; (ii) that HuDbf4 gene consists of 12 exons, which spread over a 33-kb region.

show abstract

Multiple clustered genes of the haemoglobin VIIB subfamily of Chironomus thummi thummi (Diptera)

Cited by 23 publications

References 14 publications

Intron-containing globin genes in the insect Chironomus thummi

Intron-containing globin genes in the insect Chironomus thummi

The boll weevil vitellogenin gene: Nucleotide sequence, structure, and evolutionary relationship to nematode and vertebrate vitellogenin genes

Human Dbf4/ASK promoter is activated through the Sp1 and MluI cell-cycle box (MCB) transcription elements

Contact Info

Product

Resources

About