Multiple cis Regulatory Elements for Maximal Expression of the Cauliflower Mosaic Virus 35S Promoter in Transgenic Plants

Fang, Rongxiang; Nagy, Ferenc; Sivasubramaniam, Shanthi; Chua, Nam‐Hai

doi:10.2307/3869070

Cited by 112 publications

(163 citation statements)

References 0 publications

Supporting

Mentioning

148

Contrasting

Unclassified

Order By: Relevance

“…The as-7 element was shown to be the primary determinant for the transcriptional activity of the -90 35s promoter Fang et al, 1989;Lam et al, 1989). Furthermore, nuclear extracts have been shown to contain a sequence-specific as-7 binding activity, designated ASF-1 .…”

Section: A 21-bp As-1 Element 1s Sufficient To Confer the Sa Responsementioning

confidence: 99%

Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-1 element.

et al. 1994

View full text Add to dashboard Cite

Transgenic tobacco plants carrying a number of regulatory sequences derived from the cauliflower mosaic virus 35s promoter were tested for their response to treatment with salicylic acid (SA), an endogenous signal involved in plant defense responses. PGlucuronidase (GUS) gene fusions with the full-length (-343 to +8) 35s promoter or the -90 truncation were found to be induced by SA. Time course experiments revealed that, in the continuous presence of SA, the -90 promoter construct (-90 35s-GOS) displayed rapid and transient induction kinetics, with maximum RNA levels at 1 to 4 hr, which declined to low levels by 24 hr. lnduction was still apparent in the presence of the protein synthesis inhibitor cycloheximide (CHX). Moreover, mRNA levels continued to accumulate over 24 hr rather than to decline. By contrast, mRNA from the endogenous pathogenesis-related protein-ia (PR-la) gene began to accumulate at later times during SA treatment and steadily increased thmugh 24 hr; transcription of this gene was almost completely blocked by the presence of CHX. Further dissection of the region from -90 and -46 of the 35s promoter revealed that the SAresponsive element corresponds to the previously characterized activation sequence-1 (as-í). These results represent a definitive analysis of immediate early responses to SA, relative to the late induction of PR genes, and potentially elucidate the early events of SA signal transduction during the plant defense response.

show abstract

Section: A 21-bp As-1 Element 1s Sufficient To Confer the Sa Responsementioning

confidence: 99%

Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-1 element.

et al. 1994

View full text Add to dashboard Cite

show abstract

“…This minimal promoter only drives expression of the reporter gene when there is a closely linked enhancer [24]. The expression patterns of the GUS reporter gene from TinII-(−60)-GUS, TinII-GUS, and two of the three (−60)-GUS lines were as expected, but only very weak expression was detected in one (−60)-GUS transgenic plant line ( Figure 2C, lane 9).…”

Section: Discussionmentioning

confidence: 73%

TinII intron, an enhancer to affect the function of the cytoplasmic male sterility related gene T in Brassica juncea

Jin

Cao

et al. 2013

Sci. China Life Sci.

View full text Add to dashboard Cite

The T gene, which was cloned from the mitochondria of tumorous stem mustard (Brassica juncea var. tumida), is a cytoplasmic male sterility (CMS)-related gene that can produce two transcripts, T1170 and T1243. The latter is transcribed with the uncleaved intron TinII. In our previous study, transgenic Arabidopsis thaliana plants over-expressing the T1243 transcript (OE-T1243) showed a severe male-sterile phenotype, whereas OE-T1170 plants did not. However, the functional mechanism of the T gene in B. Juncea remained unknown. In this study, microscopic analyses of paraffin sections of anthers confirmed that OE-T1243 plants did not produce normal pollen, whereas OE-T1170 plants did. We analyzed the transcription of 15 anther development-related genes and found that transcript levels of nozzle/sporocyteless and barely any meristem 1 and 2 were markedly lower in OE-T1243 plants than those in wild type, while the transcript levels of these genes in OE-T1170 plants were unchanged. To investigate the potential roles of TinII, we inserted the TinII sequence upstream of a minimal region (−60) of the cauliflower mosaic virus 35S promoter fused to the 5′ end of the β-glucuronidase (GUS) reporter gene. Analysis of the transgenic plants suggested that TinII acted as an enhancer to significantly increase GUS expression. The potential action mechanism is that the TinII intron acts as an enhancer to affect the function of the CMS-related gene T. cytoplasmic male sterility (CMS), alternative splicing, enhancer, intron Citation:Jin Z P, Wu L L, Cao J S, et al. TinII intron, an enhancer to affect the function of the cytoplasmic male sterility related gene T in

show abstract

“…This assumption is strongly supported by the result that the two regions share the two consensus sequences, 'GTGGAAA' and 'GAAGA'. The former motif is similar to the core sequence ('GTGGAAAG') of the SV40 enhancer (Fang et al 1989;Weiher et al 1983), whose binding affinity to plant DNA binding factors is unclear. The fact that the Ϫ273 to Ϫ255 region did not effectively compete for the binding of Ϫ275 to Ϫ250 and Ϫ149 to Ϫ124 probes suggests that other sequence motifs are also required.…”

Section: Forming Complexes Of 35s Enhancer Probes With Gentian Nucleamentioning

confidence: 99%

De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian

Yamasaki

Oda

Koizumi

et al. 2011

Plant Biotechnology

View full text Add to dashboard Cite

We have found that cauliflower mosaic virus (CaMV) 35S promoter-specific transgene silencing is mediated by DNA methylation in gentian (Gentiana triflora ϫ G. scabra). De novo methylation of asymmetric cytosines (CpHpH; where H is A, C, or T) sequence has been detected at the enhancer region (Ϫ148 to Ϫ85) of the 35S promoter in transgenic gentians, and is thought to be responsible for the silencing mechanism. To clarify the concept of de novo methylation, the present study examined the detailed DNA methylation profile of the entire T-DNA sequence (ca. 4 kb) integrated into transgenic gentians. Although highly methylated cytosines at CpG and CpWpG (W is A or T) sequences were broadly distributed, except in the sGFP coding region, highly methylated cytosines at CpHpH and CpCpG sequences were mainly limited to the 35S enhancer region. In addition to the previously identified de novo methylation peak (Ϫ148 to Ϫ85), another peak was discovered at Ϫ298 to Ϫ241. Electrophoretic mobility shift assays showed that gentian nuclear extracts could bind to the corresponding probes (Ϫ149 to Ϫ124 and Ϫ275 to Ϫ250), and that the probes could compete with one another for binding. Thus, a nuclear factor might be involved in the de novo methylation of the two regions. In addition, the present data indicated that the methylation patterns at CpCpG sites could be categorized as CpHpH methylation rather than CpWpG methylation.

show abstract

Multiple cis Regulatory Elements for Maximal Expression of the Cauliflower Mosaic Virus 35S Promoter in Transgenic Plants

Cited by 112 publications

References 0 publications

Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-1 element.

Immediate early transcription activation by salicylic acid via the cauliflower mosaic virus as-1 element.

TinII intron, an enhancer to affect the function of the cytoplasmic male sterility related gene T in Brassica juncea

De novo DNA methylation of the 35S enhancer revealed by high-resolution methylation analysis of an entire T-DNA segment in transgenic gentian

Contact Info

Product

Resources

About