Multiple cag genotypes of Helicobacter pylori isolates colonize the oesophagus in individual hosts in a Venezuelan population

Abstract

These results demonstrate colonization with multiple H. pylori isolates in the oesophageal mucosa, like those found in the stomach of individual hosts. H. pylori was characterized by a dominant partial island, low interleukin 8 induction with lower histopathological damage and lower antibiotic resistance, suggesting that the microenvironmental changes in individual hosts select less virulent isolates in the oesophagus than in the stomach. New approaches to ensure effective eradication therapy in multi-resistant H. pylori strains must be developed.

Point Mutations at gyrA and gyrB Genes of Levofloxacin-Resistant Helicobacter pylori Isolates in the Esophageal Mucosa from a Venezuelan Population

“…Subjects were selected from those having matching H. pylori-positive cultures of biopsies of both gastric and esophageal mucosae from a previous study (8 out of 80 dyspeptic patients) taken at the gastroenterology unit of the Oncology Service Hospital of Instituto Venezolano de los Seguros Sociales (IVSS). 5,6 All volunteers signed an informed consent form in accordance with the bioethics committees of Instituto Venezolano de Investigaciones Científicas (IVIC) and IVSS. During endoscopy procedure, antral and esophageal biopsy specimens were collected and submitted for H. pylori culture as previously described.…”

“…During endoscopy procedure, antral and esophageal biopsy specimens were collected and submitted for H. pylori culture as previously described. 6 Patients were selected from those H. pylori-positive in both gastroesophageal biopsies and culture. 5,6 Antimicrobial susceptibility testing by E-test had previously demonstrated a high LVX resistance rate in 96 isolates of the eight patients: 48 isolates from the gastric mucosa (40%) and 48 isolates from the esophageal mucosa (19%).…”

“…Helicobacter pylori in the esophageal mucosa was characterized by a partial cag-PAI island and low antibiotic resistance, whereas a higher antibiotic resistance and a complete island were found in the gastric mucosa. 6 Helicobacter pylori has a remarkable genetic variability and high allelic diversity, determining that isolates from different patients and/or same individual may have distinct genotypes; this is driven by an elevated mutation rate and frequent intraspecific recombination that contribute to host adaptation by the increment of antibiotic resistance probabilities. 7 Antibiotic resistance is a major problem in the treatment of H. pylori as it is the principal reason for the failure of eradication regimens (20-30%).…”

“…11,12 High resistance to LVX has been confirmed in H. pylori isolates from the gastroesophageal mucosa by E-test. 6 The present study aimed to investigate point mutations in the QRDR of gyrA and gyrB genes of LVX-resistant H. pylori isolates from the gastric and esophageal mucosa in individual Venezuelan patients.…”

Increased Red Blood Cell Volume Distribution Width: Important Clinical Implications in Predicting Gastric Diseases

“…Gastric diseases cause serious damage to one's health and affects one's quality of life. Today, helicobacter pylori (H. pylori) infection [4], non-steroid anti-inflammatory drug (NSAID) use [5], perceived stress [6], and smoking are considered the main causes of gastric disease. However, the mechanisms of gastric diseases remain completely unknown.…”