Multiple Brain Tumors of Diffuse Large B Cell Lymphoma in a Patient with Waldenstroem's Macroglobulinemia/Lymphoplasmacytic Lymphoma: PCR and DNA Sequence Analysis Show Evidence of Differences in Clonality of the Two B Cell Malignancies

B cell monoclonality of intraocular lymphoma and breast lymphoma

“…DNA was extracted from the vitrectomy sample and from paraffin-embedded breast tissues obtained earlier, and seminested PCR with primers for the CDR III locus of IgH was performed 10. The same-sized bands were detected in both samples (fig 1C), and direct sequencing of the PCR products revealed identical monoclonal rearrangements of the IgH gene.…”

Clonal relationship between Hashimoto thyroiditis and thyroid lymphoma

“…These include those based on the identical band size of the rearranged genes, e.g. T-cell receptor-γ and/or -β gene,18 19 IgH sequence-base analysis of sequence similarity,20 – 22 combining the two approaches, i.e. both IgH sequence and band size analysis approaches,23 digestion of chromosomal DNA samples with “rare-site” restriction endonucleases followed by pulsed field gel electrophoresis,24 mutation aberrations and loss of heterozygozity profile analysis,25 and comparative genomic hybridisation analysis 26.…”

“…Clonal similarity based on sequence analysis of IgH VDJ rearrangements has been studied in multiple tumours. For example, sequence comparison analysis of the variable (V H ) region of IgH rearrangement was used to establish the clonal relationship in Richter’s transformation of chronic lymphocytic leukaemia20 21 and DLCL transformation of lymphoplasmacytic lymphoma 22. The clonal relationship between FL and DLCL has been supported by identical sequence of the Bcl-2/IgH rearrangement 27…”

Paravertebral Waldenstrom's macroglobulinaemia presenting as pseudoclaudication

“…Tojo et al reported a paravertebral mass in a patient with WM. This case occurred in the context of advanced disease and a coexistent B-cell lymphoma 5. Our case presented with lumbosacral pseudoclaudication due to invasion of WM lymphoplasmacytic cells into the paravertebral soft tissues impinging on the neuroforamina.…”