Multiple biological activities and biosynthesis mechanisms of specific conjugated linoleic acid isomers and analytical methods for prospective application

Li, Suhong; Xu, Li; Qing, Junjun; Wu, Xiaodan; Li, Haixing; Chen, Hongbing; Liu, Xiaohua

doi:10.1016/j.foodchem.2022.135257

Cited by 21 publications

(8 citation statements)

References 113 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several strains in the genus Lactobacillus , which was increased in recipients of diet A microbiota, can saturate PUFA 17 . Metabolic intermediates in this process are known to promote metabolic health 38 , 39 . Finally, we found that differences in vena porta bile acid in mice fed diet A and MF were largely transmitted through gut microbiota transfer.…”

Section: Discussionmentioning

confidence: 99%

The interplay between dietary fatty acids and gut microbiota influences host metabolism and hepatic steatosis

Schoeler,

Ellero-Simatos,

Birkner

et al. 2023

Nat Commun

View full text Add to dashboard Cite

Dietary lipids can affect metabolic health through gut microbiota-mediated mechanisms, but the influence of lipid-microbiota interaction on liver steatosis is largely unknown. We investigate the impact of dietary lipids on human gut microbiota composition and the effects of microbiota-lipid interactions on steatosis in male mice. In humans, low intake of saturated fatty acids (SFA) is associated with increased microbial diversity independent of fiber intake. In mice, poorly absorbed dietary long-chain SFA, particularly stearic acid, induce a shift in bile acid profile and improved metabolism and steatosis. These benefits are dependent on the gut microbiota, as they are transmitted by microbial transfer. Diets enriched in polyunsaturated fatty acids are protective against steatosis but have minor influence on the microbiota. In summary, we find that diets enriched in poorly absorbed long-chain SFA modulate gut microbiota profiles independent of fiber intake, and this interaction is relevant to improve metabolism and decrease liver steatosis.

show abstract

Section: Discussionmentioning

confidence: 99%

The interplay between dietary fatty acids and gut microbiota influences host metabolism and hepatic steatosis

Schoeler,

Ellero-Simatos,

Birkner

et al. 2023

Nat Commun

View full text Add to dashboard Cite

show abstract

“…Despite the 163°C GC temperature regimen with SIC treatment facilitating the detection of 15 t-18:1, effective differentiation between the 6 t/7 t/8 t- to 11 t-18:1 isomers remained challenging ( 17 ). While GC–MS is an effective method for determining fatty acid structures, its application in the analysis of TFAs isomers is limited due to potential isomerization under high electron impact energy ( 31 ).…”

Section: Resultsmentioning

confidence: 99%

“…15 t-18:1, effective differentiation between the 6 t/7 t/8 t-to 11 t-18:1 isomers remained challenging (17). While GC-MS is an effective method for determining fatty acid structures, its application in the analysis of TFAs isomers is limited due to potential isomerization under high electron impact energy (31). In this study, SIC fractionation for the removal of cis-FAs from mixed fats was evaluated to enhance the determination of VA and EA contents in margarine, butter, cheeses, and ice creams using GCxGC-TOFMS (Table 4).…”

Section: Effect Of Sic Treatment On Va and Ea Contentsmentioning

confidence: 99%

Analysis of Vaccenic and Elaidic acid in foods using a silver ion cartridge applied to GC × GC-TOFMS

Yoo,

Lee,

Lee

et al. 2024

Front. Nutr.

View full text Add to dashboard Cite

BackgroundTrans fatty acids (TFAs) are unsaturated fatty acids, with vaccenic acid (VA) and elaidic acid (EA) being the major constituents. While VA has been associated with beneficial effects on health and anti-cancer properties, EA is found in hardened vegetable oils and is linked to an increased risk of cardiovascular diseases. Therefore, this study aimed to develop a novel method for the quantitative measurement of VA and EA, aiming to accurately analyze individual TFA and apply it for the assessment of products containing TFAs.MethodsThe ratio of VA to EA (V/E ratio) was evaluated using a silver ion cartridge (SIC) solid phase extraction method removing cis-fatty acids (cis-FAs). Additionally, comparative analysis of the V/E ratio was conducted by the two methods (SIC treatment and untreated) using comprehensive two-dimensional gas chromatography combined with time-of-flight mass spectrometry (GC × GC-TOFMS).ResultsThe removal efficiency of cis-FAs was greater than 97.8%. However, the total TFA contents were not so different from SIC treatment. Moreover, this approach not only allowed for a more precise determination of the V/E ratio but also revealed a significant distinction between natural trans fatty acids (N-TFAs) and hydrogenated trans fatty acids (H-TFAs).ConclusionTherefore, the SIC coupled to the GC × GC-TOFMS presented in this study could be applied to discriminate N-TFA and H-TFA contents in dairy and fatty foods.

show abstract

“…It is very essential for human nutrition because its synthesis process cannot be done by the body. Linoleic acid greatly reduces the risk of cardiovascular diseases, promotes healthy skin and hair, improves the health of the brain, strengthens the body's immune function, protects bone density, reduces inflammation in joints, digestive system, lungs and transplanted brain, etc 49 , 50 .…”

Section: Discussionmentioning

confidence: 99%

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Ebadi,

Ahmadi,

Tahmouresi

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Endophytic fungi are microorganisms that are considered as a potential source of natural compounds, and can be applied in various industries. The aims of this research were molecular identification of endophytic fungi isolated from the Gundelia tournefortii stems, and investigation their biological activities as well as phenolic and fatty acid profile. Surface sterilized stems of G. tournefortii were placed on potato dextrose agar (PDA) to isolate the fungal endophytes. Genomic DNA was extracted by CTAB method, and PCR amplification was performed by ITS 1 and ITS 4 as primers. The enzyme production of endophytic fungi was determined based on the formation of a clear zone that appeared around the colonies of fungus. The anti-oxidant activity was evaluated by measuring the amount of free radicals DPPH. Also, the total phenol and flavonoid contents were measured obtained by Folin-Ciocalteu and aluminum chloride colorimetric methods, respectively. Moreover, the separation and identification of phenolic acids and fatty acids were done by HPLC and GC, respectively. Phylogenetic analysis was done based on the Internal Transcribed Spacer (ITS) region, and five isolates were identified as following: Aspergillus niger, Penicillium glabrum, Alternaria alternata, A. tenuissima, and Mucor circinelloides. Evaluation of the enzymatic properties showed that P. gabrum (31 ± 1.9 mm), and A. niger (23 ± 1.7) had more ability for producing pectinase and cellulase. The anti-oxidant activity of isolates showed that A. alternata extract (IC50 = 471 ± 29 µg/mL) had the highest anti-oxidant properties, followed by A. tenuissima extract (IC50 = 512 ± 19 µg/mL). Also, the extract of A. alternata had the greatest amount of total phenols and flavonoids contents (8.2 ± 0.4 mg GAL/g and 2.3 ± 0.3 mg QE/g, respectively). The quantification analysis of phenolic acid showed that rosmarinic acid, para-coumaric acid, and meta-coumaric acid (42.02 ± 1.31, 7.53 ± 0.19, 5.41 ± 0.21 mg/g, respectively) were the main phenolic acids in the studied fungi. The analysis of fatty acids confirmed that, in all fungi, the main fatty acids were stearic acid (27.9–35.2%), oleic acid (11.3–17.3%), palmitic acid (16.9–23.2%), linoleic acid (5.8–11.6%), and caprylic acid (6.3–10.9%). Our finding showed that endophytic fungi are a source of bioactive compounds, which could be used in various industries. This is the first report of endophytic fungi associated with G. tournefortii, which provides knowledge on their future use on biotechnological processes.

show abstract

Multiple biological activities and biosynthesis mechanisms of specific conjugated linoleic acid isomers and analytical methods for prospective application

Cited by 21 publications

References 113 publications

The interplay between dietary fatty acids and gut microbiota influences host metabolism and hepatic steatosis

The interplay between dietary fatty acids and gut microbiota influences host metabolism and hepatic steatosis

Analysis of Vaccenic and Elaidic acid in foods using a silver ion cartridge applied to GC × GC-TOFMS

Investigation the biological activities and the metabolite profiles of endophytic fungi isolated from Gundelia tournefortii L.

Contact Info

Product

Resources

About