Multiple Bacteriocin Production by Leuconostoc mesenteroides TA33a and Other Leuconostoc/Weissella Strains

Abstract

Leuconostoc (Lc.) mesenteroides TA33a produced three bacteriocins with different inhibitory activity spectra. Bacteriocins were purified by adsorption/desorption from producer cells and reverse phase high-performance liquid chromatography. Leucocin C-TA33a, a novel bacteriocin with a predicted molecular mass of 4598 Da, inhibited Listeria and other lactic acid bacteria (LAB). Leucocin B-TA33a has a predicted molecular mass of 3466 Da, with activity against Leuconostoc/Weissella (W.) strains, and appears similar to mesenterocin 52B and dextranicin 24, while leucocin A-TA33a, which also inhibited Listeria and other LAB strains, is identical to leucocin A-UAL 187. A survey of other known bacteriocin-producing Leuconostoc/Weissella strains for the presence of the three different bacteriocins revealed that production of leucocin A-, B- and C-type bacteriocins was widespread. Lc. carnosum LA54a, W. paramesenteroides LA7a, and Lc. gelidum UAL 187-22 produced all three bacteriocins, whereas W. paramesenteroides OX and Lc. carnosum TA11a produced only leucocin A- and B-type bacteriocins.

Genetic characterisation and heterologous expression of leucocin C, a class IIa bacteriocin from Leuconostoc carnosum 4010

“…However, the contradiction between the calculated molecular mass according to the sequence and the mass spectrometry results (4597-4598 Da) revealed that more residues were missing (Papathanasopoulos et al 1997). Five years later, the complete amino acid sequence of mature LecC was determined, and its theoretical molecular weight (4596 Da) was closely consistent with the previous experimental value (Papathanasopoulos et al 1997;Fimland et al 2002b). Pediocin-like bacteriocins are translated as inactive precursors with Nterminal leader peptide mediating exportation of the bacteriocins ).…”

“…gelidum UAL187, have been reported to produce different class II bacteriocins, namely leucocin A (class IIa), leucocin B (class IId) and leucocin C (class IIa) van Belkum and Stiles 1995;Papathanasopoulos et al 1997). Ln.…”

Sequence and structural relationships of leucocins A-, B- and C-TA33a from Leuconostoc mesenteroides TA33a

“…Bacteriocin activity assay. Bacteriocin activity was assayed using the agar well diffusion method, as described previously (Papathanasopoulos et al, 1997;.…”

“…Leucocins A-, B-and C-TA33a were purified by adsorption/desorption of bacteriocins from the producer cells of L. mesenteroides TA33a, followed by reversed-phase HPLC, as described previously (Papathanasopoulos et al, 1997;Yang et al, 1992). Aliquots of each purified preparation were lyophilized for further analysis.…”

“…Recently, Leuconostoc mesenteroides TA33a was found to produce three different bacteriocins with different antimicrobial activity spectra (Papathanasopoulos et al, 1997). This paper reports on the primary structures of leucocins A-, B-and C-TA33a produced by L. mesenteroides TA33a and a comparison of the conformations adopted by these peptides in membrane-mimicking environments.…”