Multiple Argonaute family genes contribute to the siRNA-mediated RNAi pathway in Locusta migratoria

Wang, Yanli; Fan, Yunhe; Abbas, Mureed; Ma, Enbo; Cooper, Anastasia M.W.; Silver, Kristopher; Zhu, Kun Yan; Zhang, Jianzhen

doi:10.1016/j.pestbp.2020.104700

Cited by 16 publications

(12 citation statements)

References 48 publications

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“…However, up-regulation of OfAgo1 was observed 2 h post-injection of ds EGFP , which implies that OfAgo1 could also be involved in siRNA pathway at an early stage of dsRNA exposure in O. furnacalis. These results are similar to the functions of Ago1 in L. migratoria, which has been shown to be involved in siRNA-mediated RNAi pathway [ 31 ]. However, further research is needed to clarify the role of OfAgo1 in siRNA-mediated RNAi pathway.…”

Section: Discussionsupporting

confidence: 82%

“…A previous study conducted by Tomoyasu et al [ 30 ] has shown that two Ago2 ( TcAgo2a and TcAgo2b ) genes identified from the T. castaneum genome are involved in systemic RNAi response of RNAi. Similarly, both LmAgo2a and LmAgo2b have been found to contribute to the robust RNAi response in L. migratoria [ 31 ]. In L. decemlineata, two Dicer2 genes ( Dicer2a and Dicer2b ) and two Ago2 genes ( Ago2a and Ago2b ) were identified in L. decemlineata transcriptome database [ 23 ], which support the hypothesis that the number of core RNAi pathway genes may contribute to the variability of RNAi efficiency in insects.…”

Section: Discussionmentioning

confidence: 99%

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Increased RNAi Efficiency by dsEGFP-Induced Up-Regulation of Two Core RNAi Pathway Genes (OfDicer2 and OfAgo2) in the Asian Corn Borer (Ostrinia furnacalis)

Fan

Abbas

Liu

et al. 2022

Insects

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RNA interference (RNAi) is a sequence-specific gene silencing mechanism that holds great promise for effective management of agricultural pests. Previous studies have shown that the efficacy of RNAi varies among different insect species, which limits its wide spread application in the field of crop protection. In this study, we identified and characterized six core RNAi pathway genes including OfDicer1, OfDicer2, OfR2D2, OfAgo1, OfAgo2, and OfAgo3 from the transcriptomic database of the Asian corn borer (Ostrinia furnacalis). Domain analysis showed that the six deduced proteins contained the necessary functional domains. Insect developmental stage- and tissue-specific expression analysis showed that five genes were expressed in all the stages and tissues examined except OfAgo3, which showed low expression in larvae, and high expression in pupae and adults and in the midgut. RT-qPCR was performed to examine the response of these six genes to exogenous double-stranded RNA (dsRNA). Interestingly, the transcript levels of OfDicer2 and OfAgo2 were significantly enhanced after the injection of dsEGFP at different time points and tissues investigated. Consequently, the RNAi efficiency in targeting the insect endogenous genes can be greatly enhanced in the hemolymph or midgut. Taken together, our investigations suggest that RNAi efficiency can be enhanced by pre-injection of dsRNA to induce the RNAi core machinery genes, which could be a useful strategy to improving RNAi efficiency for studying gene functions under laboratory conditions.

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Section: Discussionsupporting

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Increased RNAi Efficiency by dsEGFP-Induced Up-Regulation of Two Core RNAi Pathway Genes (OfDicer2 and OfAgo2) in the Asian Corn Borer (Ostrinia furnacalis)

Fan

Abbas

Liu

et al. 2022

Insects

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“…The expression level of Ago1 was the highest in the ovary followed by the fat body in the spatial expression. Ago1 in Z. cucurbitae shows a consistent mRNA expression pattern with its homologs ( Gao et al, 2020 ). Gawky expression was the highest in eggs followed by an adult in temporal expression, while in spatial expression, its level was the highest in the head of Z. cucurbitae .…”

Section: Discussionmentioning

confidence: 85%

Argonaute1 and Gawky Are Required for the Development and Reproduction of Melon fly, Zeugodacus cucurbitae

et al. 2022

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Argonaute family genes encode a highly conserved group of proteins that have been associated with RNA silencing in both animals and plants. This study investigates the importance of microRNA biogenesis key regulators Argonaute1 (Ago1) and Gawky genes in the post-embryonic and ovarian development of the melon fly, Zeugodacus cucurbitae. The expression levels of these genes were mapped in all developmental stages and different adult tissues. Their roles in development were investigated using RNA interference (RNAi) via two different dsRNA delivery techniques. Embryo microinjection and oral feeding of third instar larvae successfully knocked down and greatly reduced the expression level of the target genes. Additionally, ex vivo essays revealed the stability of dsRNA in food was sufficient for gene silencing, although its integrity was affected in midgut. A wide range of phenotypes were observed on pupation, segmentation, pigmentation, and ovarian development. RNAi-mediated silencing of Gawky caused high mortality and loss of body segmentation, while Ago1 knockdown affected ovarian development and pigmentation. Developmental abnormalities and ovarian malformations caused by silencing these genes suggest that these genes are crucial for viability and reproductive capacity of Z. cucurbitae, and may be used as potential target genes in pest management.

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“…Argonautes (AGO) are important core proteins in the RNA interference (RNAi) pathway of eukaryotic cells. There are mainly four AGO members (AGO1-4) in humans, which are expressed in cells and tissues [ 37 ]. In human cells, tRF is associated with Argonaute 1, 3, and 4, but not with Argonaute 2.…”

Section: Discussionmentioning

confidence: 99%

Differential Expression and Bioinformatics Analysis of tRF/tiRNA in Endometriosis Patients

Wangshu¹,

Li²,

Cao³

et al. 2022

BioMed Research International

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Background. Endometriosis (EMs) is a benign chronic condition that tends to recur in women of childbearing age, with an incidence of approximately 10%. It is a multifactorial disease for which the pathogenesis is currently unclear. This study is aimed at investigating the expression and clinical significance of tRNA-derived small RNA (tsRNA), a novel noncoding small RNA with potential regulatory functions, in endometriosis. Methods. The tRF/tiRNA expression profiles in endometrial tissues from three pairs of endometriosis patients and controls were detected by tRF&tiRNA PCR microarray technology and then verified by quantitative real-time polymerase chain reaction (qPCR). The target genes and target sites of TRF396, tiRNA-5030-GlnTTG-3, TRF308, and TRF320 were predicted by miRanda, and the network diagram of their interaction with miRNA was drawn. The impact of tRNA-derived fragments on the pathogenesis of endometriosis was analyzed using the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG). Results. Two upregulated and 19 downregulated tRNA-derived fragments were identified. The qRT-PCR results of 2 upregulated and 2 downregulated RNA-derived fragments were consistent with the RNA Seq data. The OR2B4 gene related to TRF396, the DGAT1 gene related to tiRNA-5030-GlnTTG-3, the KLF16 gene of TRF308, and the RNF213 gene of TRF320 had significant correlations. Gene Ontology and pathway analysis showed that the target genes of TRF396 and tiRNA-5030-GlnTTG-3 were mainly involved in the intrinsic components of the membrane and the overall composition of the membrane in cell components; molecular functions mainly involve olfactory conduction and G protein-coupled receptor activity. In the biological process, it was mainly involved in the detection of sensory stimuli. The target genes of TRF308 and TRF320 were mainly involved in the intracellular part; molecular functions are mainly related to DNA binding transcription factor activity and protein binding and mainly related to biological regulation of biological processes. Pathway analysis showed that the RAP1 signaling pathway and the AXON GUIDANCE signaling pathway may participate in the progression of endometriosis. Conclusion. The differential expression of tRF/tiRNA in endometriosis may be related to the pathogenesis of endometriosis. Furthermore, tRF/tiRNA may be a biomarker for the diagnosis and treatment of EMs in the future.

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Multiple Argonaute family genes contribute to the siRNA-mediated RNAi pathway in Locusta migratoria

Cited by 16 publications

References 48 publications

Increased RNAi Efficiency by dsEGFP-Induced Up-Regulation of Two Core RNAi Pathway Genes (OfDicer2 and OfAgo2) in the Asian Corn Borer (Ostrinia furnacalis)

Increased RNAi Efficiency by dsEGFP-Induced Up-Regulation of Two Core RNAi Pathway Genes (OfDicer2 and OfAgo2) in the Asian Corn Borer (Ostrinia furnacalis)

Argonaute1 and Gawky Are Required for the Development and Reproduction of Melon fly, Zeugodacus cucurbitae

Differential Expression and Bioinformatics Analysis of tRF/tiRNA in Endometriosis Patients

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