Multiple Applications of Endophytic Colletotrichum Species Occurring in Medicinal Plants

Rai, Mahendra; Agarkar, Gauravi; Rathod, Dnyaneshwar

doi:10.1002/9781118460566.ch18

Cited by 15 publications

(9 citation statements)

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“…Endophytic fungi inhibit plants having a broad spectrum of environmental habitats and thus require diverse adaptative potentials due to their exposure to these unusual environments. These characteristics permit them to be ideal candidates for bio-control processes and thus are applicable in pharmaceuticals as well as medical and agricultural industries (Rai et al 2014;Gouda et al 2016;Fareed et al 2017). In addition, endophytic fungi are generally known to produce secondary metabolites (4-hydroxybenzoic acid, indole-3-acetic acid (IAA) and gibepyrone D) that exhibit biological activities against a wide variety of microbes (Deshmukh et al 2014;Bogner et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Antibacterial activity of endophytic fungi isolated from Sceletium tortuosum L. (Kougoed)

et al. 2019

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Endophytic fungi have the ability to co-exist with their host plants without causing any harm and are beneficial to both the plant and the fungi. The current study determined the antimicrobial properties and identify the chemical compounds of secondary metabolites produced by endophytic fungi isolated from Sceletium tortuosum L. A total of 60 endophytic fungi produced secondary metabolites that were detected after fermentation and extraction. Antibacterial properties of the secondary metabolites were determined using the disc diffusion assay against pathogenic environmental Gram-positive and Gram-negative bacteria as well as control stains. The chemical compounds were characterized by GC-MS. Overall, 15% of fungal extracts displayed narrow spectrum of activity against the bacteria strains. Despite this, none of the fungal extracts inhibited growth of Enterococcus faecalis (ATCC S1299) and Enterococcus gallinarum (ATCC 700425) while Bacillus cereus (ATCC 10876) was the most susceptible against the fungal extracts. Fusarium oxysporum (GG 008) with accession no. KJ774041.1 displayed significant antibacterial activity that was linked to high levels of 5-hydroxymethylfurfural (HMF) and octadecanoic acid as revealed by GC-MS. This study revealed the presence of bioactive secondary metabolites with antibacterial activities from fungi isolated from Sceletium tortuosum L.

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Section: Resultsmentioning

confidence: 99%

Antibacterial activity of endophytic fungi isolated from Sceletium tortuosum L. (Kougoed)

et al. 2019

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“…One such mechanism is to explore the biotechnological potential of the microbial communities residing within the floral resources that are commonly referred to as endophytes. Endophytes have been proven to show positive effects on the host plants by serving as growth promoter, insect and pest repellents, antimicrobial agents against plant pathogens and stress modulators (Ryan et al 2008 ; Staniek et al 2008 ; Nagabhyru et al 2013 ; Rai et al 2014 ). A deeper look on endophytes also revealed that they are a source of various bioactive substances that find importance to ecology, medicine, pathology and agriculture (Wang et al 2008 ).…”

Section: Introductionmentioning

confidence: 99%

Culturable endophytic bacteria associated with medicinal plant Ferula songorica: molecular phylogeny, distribution and screening for industrially important traits

et al. 2016

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Xinjiang, a region of high salinity and drought, is a host to many arid and semi-arid plants. Many of these plants including Ferula spp. have indigenous pharmaceutical histories. As many of the medicinal properties of plants are in tandem with the associated microorganisms residing within the plant tissues, it is advisable to explore the endophytic potential of such plants. In the present study, diversity of culturable bacteria isolated from medicinal plants Ferula songorica collected from Hebukesaier, Xinjiang were analyzed. A total of 170 endophytic bacteria belonging to three phyla, 15 orders, 20 families and 27 genera were isolated and characterized by 16S rRNA gene sequencing. The phylum Actinobacteria constitutes a major portion of the endophytic microbes isolated from the plant Ferula songorica (107 isolates). Overall endophytic species richness of the sample was 58 taxa while the sample has statistical values of 4.02, 0.97, 0.65 and 16.55 with Shannon’s, Simpson, Species evenness and Margalef, respectively. Root tissues were found to be more suitable host for endophytes as compared to leaf and stem tissues. Among these endophytic strains, 88 % can grow on nitrogen-free media, 19 % solubilize phosphate, while 26 and 40 % are positive for production of protease and cellulase, respectively. The results confirm that the medicinal plant Ferula songorica represents an extremely rich reservoir for the isolation of diverged bacteria with potential for growth promoting factors and biologically active compounds including enzymes.

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“…and Curvularia sp. of several medicinal plants 23,25,26 . So, the isolated fungi have potentiality to produce biologically active compounds for antimicrobial agent development as these previous reports.…”

Section: Discussionmentioning

confidence: 99%

“…and Alternaria sp. of several medicinal plants 23,25,27,28,30 . However, Colletotrichum gloeosporioides isolated from leaves of C. papaya exhibited moderate antioxidant activity in the DPPH assay and moderate cytotoxic activity against cisplatin-sensitive ovarian cancer cell line 2780 (sens) and cisplatin-resistant ovarian cancer cell line 2780 (CisR) 8 .…”

Section: Discussionmentioning

confidence: 99%

Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of Carica papaya L. Leaves

Lini

Zinnurine

Rahman

et al. 2021

JNR

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Objectives: This work describes the chemical and in-vitro bioactivity studies on endophytic fungi derived from the leaves of Carica papaya L. Methods: Column Chromatography was employed for isolation of fungal metabolites. Nuclear Magnetic Resonance (NMR) spectral data was utilized to elucidate the structure of the isolated compounds and physicochemical properties of them were also examined. As a part of in-vitro bioactivity screening disc diffusion method, 1, 1-diphenyl-2- picryl-hydrazyl (DPPH) scavenging assay and brine shrimp lethality bioassay were conducted to evaluate antimicrobial, antioxidant and cytotoxic activities, respectively. Results: A total of four fungal isolates were identified as endophytic fungi and purified from the young leaves of Carica papaya. These fungi, encoded as CPLE-1, CPLE-2, CPLE-3 and CPLE-4, were identified up to the genus level on the basis of their macroscopic and microscopic characteristics as Colletotrichum sp., Carvularia sp., and next two as Alternaria sp., respectively. Chemical profiling and preliminary bioactivity screening of the fungal endophytes signified strain CPLE-3 with highest potentiality to produce bioactive compounds which directs its large scale cultivation. Ergosterol and Alternariol monomethyl ether were isolated from the ethyl acetate extract of the fungus, CPLE-3. Conclusion: Bioactivity and chemical screening of the endophytic fungal extracts of Carica papaya leaf along with the isolation of fungal secondary metabolites suggested these endophytic fungi to be possible source of bioactive leads for developing new and improved medicines.

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Multiple Applications of Endophytic Colletotrichum Species Occurring in Medicinal Plants

Cited by 15 publications

References 50 publications

Antibacterial activity of endophytic fungi isolated from Sceletium tortuosum L. (Kougoed)

Antibacterial activity of endophytic fungi isolated from Sceletium tortuosum L. (Kougoed)

Culturable endophytic bacteria associated with medicinal plant Ferula songorica: molecular phylogeny, distribution and screening for industrially important traits

Bioactivity Screening and Identification of Secondary Metabolites from Fungal Endophytes of <i>Carica papaya</i> L. Leaves

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