Multiple antibiotic resistance indexing of coliforms to identify high risk contamination sites in aquatic environment

Chitanand, M. P.; Kadam, Tukaram; Gyananath, G.; Totewad, N. D.; Balhal, D. K.

doi:10.1007/s12088-010-0042-9

Cited by 73 publications

(48 citation statements)

References 10 publications

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“…MAR range 0.1-0.7, 0.3-0.9 and 0.35-1.0 were recorded among the isolates from site I (polluted), site II (polluted) and site III (less polluted receiving the treated water near the treatment plant), respectively. No significant difference among the isolates from polluted and less polluted sites was observed regarding their antibiotic (Chitanand et al, 2010) All E. coli strains isolated from river and polluted waters show a high incidence of MAR phenotype. Many investigators have recognized that wastewater treatment plants are the principal recipients of enteric bacteria with multiple antibiotic resistance (Selvaratnum and Kuberger, 2004) and an important site for horizontal gene transfer, by containing nutrients and high concentrations of microorganisms (Sloan et al, 2014).…”

Section: Discussionmentioning

confidence: 81%

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Akhter¹,

Imran²,

Akhter³

2014

Afr. J. Microbiol. Res.

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The antibiotic resistance profiles of Escherichia coli isolated from three different sampling sites of the Gomti River at Lucknow city was evaluated. Water samples were collected and then analyzed for the presence of E. coli, using standard methods. Antibiotic susceptibility testing was performed by the disc diffusion method. Of the 77 E. coli isolates tested, marked antibiotic resistances (over 70%) were observed for amoxicillin, nitrofurozone, chloramphenicol, polymixin B, methicilin, ampicillin, nalidixic acid, cefpodoxime, erythromycin, penicillin, rifampicin and ofloxacin depending upon the sampling sites. All E. coli isolates also showed multiple resistance patterns in different combination of antibiotics. The MAR index ranges were found very high indicating the high risk of environmental contamination. The findings indicated that pollution of aquatic environments from different sources of the city may have a potential impact on the dissemination and survival of E. coli, as well as other pathogenic bacteria in the Gomti River water for public and animal health. This may result to a negative effect on antibiotic therapy for infectious diseases.

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Section: Discussionmentioning

confidence: 81%

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Akhter¹,

Imran²,

Akhter³

2014

Afr. J. Microbiol. Res.

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“…Similarly, Tambekar et al, [15] reported that the thermotolerant E.coli isolated from the drinking water from salinity affected villages of Purna River basin of Vidarbha were 75% human fecal origin and 25% were non human fecal origin. The study done by Chitanand et al, [16] showed that multiple antibiotic resistances was prominently seen in coliforms at downstream sites while it was low in coliforms at up streams sites along the bank of river and the differences in MAR indices provide a method for distinguishing high risk contamination sites in aquatic environment. The antibiotic resistance index for detected thermotolerant E.coli in ODNF (Open defecation not free) villages were found higher than in ODF (open defecation free) villages [17].…”

Section: Resultsmentioning

confidence: 99%

Microbial source tracking through antibiotic resistance indexing of Escherichia coli to identify source of fecal contamination in drinking water

2017

IJAPSA

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Fecal pollution of water leads to introduce variety of intestinal pathogens that causes water borne diseases. Microbial source tracking (MST) methods are increasingly being used to identify fecal contamination sources in drinking water A total of 400 drinking water samples were collected from the houses (200), schools (100), hostels (50) and commercially available water cans (50), out of which 160 water samples were found nonpotable. A total of 154 E.coli were isolated and identified from these 160 nonpotable drinking water samples. The 123(79%) from human fecal origin and 31(21%) animal fecal origin E. coli were found after the microbial source tracking by multiple antibiotic resistances (MAR) analysis. In hostels and School's water identified a total of 19 and 18 human fecal originated E.coli. Out of 12 E.coli isolated from 15 Can's nonpotable water; 10(83%) were human and 2(17%) animal fecal origin. The 105 E.coli were isolated from the household drinking water out of these 76(72%) was human and 29(28%) was animal fecal origin. The study concluded that the water in urban area was mainly polluted due human fecal origin as compare to animal fecal origin.

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“…Sebaliknya menurut Rocha et al (2016) sebanyak 70 strain vibrio dari sampel air dan sedimen di lingkungan tambak udang bersifat resisten terhadap salah satu antibiotik seperti siprofloksasin dan kloramfenikol. Beberapa strain bakteri yang bersifat resisten terhadap siprofloksasin merupakan permasalahan serius saat ini karena antibiotik ini merupakan pilihan antibiotik yang cukup baik untuk beberapa bakteri gram negatif (Chitanand, Kadam, Gyananath, Totewad, & Balhal, 2010).…”

Section: Pola Resistensi Bakteri Terhadap Antibiotikunclassified

“…Chitanand et al (2010) melaporkan bahwa indeks MAR kisaran rendah (0,15) di wilayah upstream mengindikasikan adanya risiko kontaminasi rendah. Sementara indeks yang moderat berada di wilayah midstream, dan indeks MAR yang lebih tinggi di wilayah down stream.…”

Section: Nilai Indeks Mar (Multiple Antibiotic Resistance)unclassified

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

Kusmarwati

Yenni

Indriati

2017

JPBKP

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ABSTRAKKeberadaan bakteri V. parahaemolyticus pada produk udang yang bersifat resisten terhadap antibiotik saat ini menjadi permasalahan serius yang berdampak pada jaminan mutu dan keamanan produk. Hal ini menjadi ancaman serius bagi manusia ketika mengkonsumsi udang tersebut. Penelitian ini bertujuan untuk mengetahui resistensi antibiotik dan potensi risiko antibiotik dari bakteri V. parahaemolyticus pada udang vaname. Pengambilan sampel udang vaname segar dari tambak dilakukan pada musim hujan dan musim kemarau di wilayah Pantai Utara Jawa pada bulan Februari hingga Oktober 2015 dengan metode purposive random sampling. Sebanyak 36 isolat bakteri V. parahaemolyticus yang mewakili 103 sampel udang vaname segar dari tambak udang di Jawa Barat, Jawa Tengah dan Jawa Timur telah diuji resistensinya terhadap 8 jenis antibiotik (doksisiklin, nitrofurantoin, siprofloksasin, asam nalidiksat, amoksisilin-asam klavulanat, kloramfenikol, streptomisin, dan eritromisin). Uji kepekaan bakteri terhadap antibiotik dilakukan menggunakan metode Kirby-Bauer. Perhitungan indeks Multiple Antibiotic Resistance (MAR) juga dilakukan untuk mengetahui potensi risiko antibiotik terhadap kesehatan manusia. Hasil analisis menunjukkan bahwa 100% isolat bakteri yang diuji resisten terhadap streptomisin, 90% isolat resisten terhadap eritromisin, dan berikutnya resisten terhadap amoksisilin-asam klavulanat dan nitrofurantoin masing-masing sebesar 83,33% dan 58,33%. Terdapat beberapa antibiotik yang masih mampu melawan bakteri V. parahaemolyticus yaitu siprofloksasin (88,89%), kloramfenikol (81,25%) dan doksisiklin (33,33%). Selain itu, sebanyak 63,89% dari total isolat bakteri tersebut memiliki indeks MAR>0,2 yang mengindikasikan adanya potensi risiko bagi kesehatan. Secara keseluruhan, bakteri V. parahaemolyticus dari sampel yang diambil pada musim hujan menunjukkan resistensi yang lebih tinggi. (doxycycline, nitrofurantoin, ciprofloxacin, nalidixic acid, amoxicillin-clavulanic acid, chloramphenicol, streptomycin, and erythromycin KATA KUNCI : multiple antibiotic resistance, udang, tambak, kloramfenikol ABSTRACT Recently, the occurence of V. parahaemolyticus bacteria in shrimp products that was resistant to antibiotics became a serious problem that affects quality assurance and product safety. It will be a serious threat to humans when consuming the shrimp product. Research was conducted to know antibiotic resistance and antibiotic risk potency of V. parahaemolyticus bacteria on vannamei shrimp. Sampling of fresh vannamei shrimp from the ponds was conducted in rainy and dry season in the Northern Coast of Java from February to October 2015 with purposive randomize sampling method. A total of 36 isolates of V. parahaemolyticus bacteria representing 103 samples of fresh vannamei shrimp from shrimp ponds in West Java, Central Java and East Java have been tested for resistance to 8 types of antibiotics

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Multiple antibiotic resistance indexing of coliforms to identify high risk contamination sites in aquatic environment

Cited by 73 publications

References 10 publications

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Determination of multiple antibiotic resistance patterns and indexing among metal tolerant -lactamase-producing Escherichia coli

Microbial source tracking through antibiotic resistance indexing of Escherichia coli to identify source of fecal contamination in drinking water

RESISTENSI ANTIBIOTIK PADA Vibrio parahaemolyticus DARI UDANG VANAME ASAL PANTAI UTARA JAWA UNTUK PASAR EKSPOR

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