Multiple and sensitive SERS detection of cancer-related exosomes based on gold–silver bimetallic nanotrepangs

Ning, Cui-Fang; Wang, Luying; Tian, Ya-Fei; Yin, Bin‐Cheng; Ye, Bang‐Ce

doi:10.1039/c9an02180a

Cited by 73 publications

(55 citation statements)

References 39 publications

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“…Aptamers (PSMA, HER2, and AFP) for three kinds of target exosomes were modified on magnetic beads. [ 42 ] The proposed SERS‐based sensor in multiplex exosomes detection is highly promising in the application of clinical diagnosis.…”

Section: Exosome Detection Methodologiesmentioning

confidence: 99%

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Xiong

Huang

Yang

et al. 2021

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Exosomes, known as nanometer‐sized vesicles (30–200 nm), are secreted by many types of cells. Cancer‐derived exosomes have great potential to be biomarkers for early clinical diagnosis and evaluation of cancer therapeutic efficacy. Conventional detection methods are limited to low sensitivity and reproducibility. There are hundreds of papers published with different detection methods in recent years to address these challenges. Therefore, in this review, pioneering researches about various detection strategies are comprehensively summarized and the analytical performance of these tests is evaluated. Furthermore, the exosome molecular composition (protein and nucleic acid) profiling, a single exosome profiling, and their application in clinical cancer diagnosis are reviewed. Finally, the principles and applications of machine learning method in exosomes researches are presented.

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Section: Exosome Detection Methodologiesmentioning

confidence: 99%

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Xiong

Huang

Yang

et al. 2021

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“…Measurement of HER2 was highly sensitive and reproducible (RSD < 7%), and EVs were detected at 50 EVs/µL with a low RSD of 6.4% [104]. Ning et al developed a reliable SERS sensor for the simultaneous detection of multiple cancer-related EVs [105]. The SERS probes consisted of Au-Ag-Ag core-shell-shell nanotrepangs (GSSNTs) and functionalized DNA with a bumpy structured surface.…”

Section: Raman-based Analysis Methods For Ev Detectionmentioning

confidence: 99%

Applications of Bionano Sensor for Extracellular Vesicles Analysis

2020

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Recently, extracellular vesicles (EVs) and their contents have been revealed to play crucial roles in the intrinsic intercellular communications and have received extensive attention as next-generation biomarkers for diagnosis of diseases such as cancers. However, due to the structural nature of the EVs, the precise isolation and characterization are extremely challenging. To this end, tremendous efforts have been made to develop bionano sensors for the precise and sensitive characterization of EVs from a complex biologic fluid. In this review, we will provide a detailed discussion of recently developed bionano sensors in which EVs analysis applications were achieved, typically in optical and electrochemical methods. We believe that the topics discussed in this review will be useful to provide a concise guideline in the development of bionano sensors for EVs monitoring in the future. The development of a novel strategy to monitor various bio/chemical materials from EVs will provide promising information to understand cellular activities in a more precise manner and accelerates research on both cancer and cell-based therapy.

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“…Ning et al. synthesized the aptamer-based SERS detection probes based on gold–silver–silver core-shell–shell nanotrepangs (GSSNTs) nanotags and magnetic beads for simultaneous detection of multiple cancer-related exosomes: the biomarkers (PSMA, Her2, and AFP proteins) for the prostate cancer cell line (LNCaP), breast cancer cell line (SKBR3), and hepatocellular cancer cell line (HepG2) ( 48 ). For the simultaneous detection of multiple kinds of exosomes (SKBR3, T84, and LNCaP), three different SERS probes types were designed to have three different types of Raman reporters and aptamers by Weng et al.…”

Section: Optical Aptasensors In Early Cancer Detectionmentioning

confidence: 99%

Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types

Zahra

Khan

2021

Front. Oncol.

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Cancer is a life-threatening concern worldwide. Sensitive and early-stage diagnostics of different cancer types can make it possible for patients to get through the best available treatment options to combat this menace. Among several new detection methods, aptamer-based biosensors (aptasensors) have recently shown promising results in terms of sensitivity, identification, or detection of either cancerous cells or the associated biomarkers. In this mini-review, we have summarized the most recent (2016–2020) developments in different approaches belonging to optical aptasensor technologies being widely employed for their simple operation, sensitivity, and early cancer diagnostics. Finally, we shed some light on limitations, advantages, and current challenges of aptasensors in clinical diagnostics, and we elaborated on some future perspectives.

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Multiple and sensitive SERS detection of cancer-related exosomes based on gold–silver bimetallic nanotrepangs

Abstract: Exosomes are endogenous vesicles of cells, and can be used as important biomarkers for cancers.

Cited by 73 publications

References 39 publications

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Recent Progress in Detection and Profiling of Cancer Cell‐Derived Exosomes

Applications of Bionano Sensor for Extracellular Vesicles Analysis

Advances in Optical Aptasensors for Early Detection and Diagnosis of Various Cancer Types

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