Multiple Allelism in Flavonoid Hydroxylation in Eustoma grandiflorum (Raf.) Shinn. Flowers

Hashimoto, Fumio; Uddin, A. F. M. Jamal; Shimizu, Kazuo; Sakata, Yusuke

doi:10.2503/jjshs.73.235

Cited by 3 publications

(6 citation statements)

References 34 publications

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“…In this study, we examined the genomic sequences of the ANS gene in lisianthus to elucidate the cause of the acyanic flower. We report that the 94-bp deletion in the different self-and cross-pollinated progenies were transplanted 110 days after sowing to a polyethylene house at Kagoshima University Experimental Farm under standard conditions as previously described (Hashimoto et al, 2004;Uddin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Shimizu

Ohnishi

Morikawa

et al. 2011

J. Japan. Soc. Hort. Sci.

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We studied the genomic sequence of the anthocyanidin synthase (ANS) gene in acyanic flower lines of lisianthus and detected a 94-bp deletion of the sequence in comparison with that of cyanic flower lines. Cross-pollination was made between acyanic and cyanic flower lines to yield F 1 progeny, followed by its self-pollination. The segregated individuals between cyanic and acyanic flowers among the F 2 population were of 24 and 8, respectively (3 : 1), which suggested that one gene of the F 1 hybrid was unequivocally involved in anthocyanin production. The homozygous ANS genes that have a set of 94-bp deletion co-segregated a recessive phenotype of white color in the F 2 population. Genomic PCR of the ANS genes of the 14 independent lisianthus lines of acyanic flowers, such as white, yellow, pale yellow, and pale green flowers, revealed that all possessed a homozygous 94-bp deletion. The data support that the acyanic flower colors are associated with a 94-bp deletion in the ANS gene as a result of the loss of anthocyanin accumulation.

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Section: Introductionmentioning

confidence: 99%

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Shimizu

Ohnishi

Morikawa

et al. 2011

J. Japan. Soc. Hort. Sci.

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“…Seeds of the lisianthus cultivars 'Asuka no Kurenai' and 'Papillon Rose Pink' were obtained from Sakata Seed Corporation (Yokohama, Japan) and Miyoshi & Co., Ltd. (Tokyo, Japan), respectively. Seedlings of the cultivars and their different self-and cross-pollinated progenies were transplanted to a polyethylene house at Kagoshima University Experimental Farm under standard conditions 110 days after sowing as described by Hashimoto et al (2004) and Uddin et al (2002).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The difference among Dp, Cy, and Pg is attributed to the variation in the hydroxylation pattern of the anthocyanin B-ring. From genetic analysis of cross-pollination in several varieties, Hashimoto et al (2004) concluded that multiple allelic genes control the B-ring hydroxylation-related anthocyanidin composition in lisianthus.…”

Section: Introductionmentioning

confidence: 99%

Flavonoid 3',5'-Hydroxylase (F3'5'H) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

et al. 2022

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This study investigated genetic polymorphism related to anthocyanin composition variation in lisianthus (Eustoma grandiflorum) flower petals. Three different bands were detected by genomic polymerase chain reaction using specific primers based on the reported flavonoid 3',5'-hydroxylase (F3'5'H) gene sequence. Our genetic study revealed that they were allelic. They were named F3'5'H1-1, F3'5'H1-2, and f3'5'h1-2. F3'5'H1-1 and F3'5'H1-2 differed in the length of the intron. Allele f3'5'h1-2 possessed a retrotransposon insertion in the first exon, but otherwise, its gene sequence was almost identical to that of F3'5'H1-2. This retrotransposon is a Ty1-copia type retrotransposon and was designated as the retrotransposon of Eustoma grandiflorum 1 (rTeg1). The mauve flower line 'ME' and red-purple flower line 'PRP' were homozygous for F3'5'H1-1 and mostly accumulated cyanidin, with a small amount of delphinidin, in flower petals (the Cy major phenotype). The violet flower line 'RV' was homozygous for F3'5'H1-2 and accumulated only delphinidin (the Dp major phenotype). The pink-red flower line 'AK' was homozygous for f3'5'h1-2, accumulated pelargonidin, and lacked delphinidin (the Pg major-non Dp phenotype). Segregation analysis of the F 2 population from 'ME' × 'RV' revealed that F3'5'H1-2 was associated with the Dp major phenotype, and the delphinidin major trait was dominant in the Cy major phenotype. All the Cy major phenotype plants in the F 2 population possessed homozygous F3'5'H1-1. Genetic analysis of the F 2 population from the cross between delphinidin-containing and delphinidin-lacking strains revealed that the homozygous f3'5'h1-2 genotype co-segregated with the Pg major-non Dp phenotype, which was recessive to the delphinidin-containing phenotypes. Expression analysis of the F3'5'H gene demonstrated that an abnormal mRNA was transcribed from the F3'5'H gene in the homozygous f3'5'h1-2 line by the insertion of rTeg1. Therefore, these F3'5'H gene polymorphisms can be used as DNA markers for breeding lisianthus based on flower color.

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“…The progeny obtained by self-pollination of these cultivars were designated as ME, RV, and AK, respectively (Shimizu et al, 2011). Lisianthus plants were grown for gene cloning and RT-PCR analysis was performed as described previously (Hashimoto et al, 2004;Shimizu et al, 2011;Uddin et al, 2002). RNA was extracted from leaves as well as petals at six different floral developmental stages: 1) bud less than 20 mm long; 2) bud between 20 mm and 30 mm; 3) bud more than 30 mm long and tightly closed; 4) bud more than 30 mm long and loosely closed; 5) opening flower; and 6) fully opened flower.…”

Section: Plant Materialsmentioning

confidence: 99%

“…The anthocyanidin compositions of lisianthus and Japanese morning glory were analyzed by HPLC using previously described procedures (Hashimoto et al, , 2004Uddin et al, 2001Uddin et al, , 2002, with some modifications. Flower petals were harvested at the fully opened stage and stored at −80°C until use.…”

Section: Characterization Of Anthocyanidin Compositionmentioning

confidence: 99%

Cloning of the Flavonoid 3'-Hydroxylase Gene of Eustoma grandiflorum (Raf.) Shinn. (EgF3'H) and Complementation of an F3'H-deficient Mutant of Ipomoea nil (L.) Roth. by Heterologous Expression of EgF3'H

et al. 2015

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A full-length cDNA of a putative flavonoid 3'-hydroxylase (F3'H) gene encoding a key enzyme in the production of cyanidin was cloned from a lisianthus (Eustoma grandiflorum) petal. Lisianthus F3'H (EgF3'H) shares 75.1, 73.8, and 68.2% amino acid identity with Arabidopsis thaliana, Ipomoea nil, and Petunia hybrida, respectively. RT-PCR revealed that wild-type lisianthus flowers accumulated higher levels of F3'H mRNA during the early stages of development than in the late stages. The accumulated F3'H transcript levels in leaves were similar to those in flowers in the early stages of development. Overexpression of lisianthus F3'H cDNA altered flower color from red to blue in the I. nil cultivar 'Violet', which lacks a functional F3'H gene. In addition, the transgenic 'Violet' plants accumulated cyanidin and peonidin at similar levels to wild-type I. nil. Taking these findings together, this study demonstrates that EgF3'H functions as a flavonoid 3'-hydroxylase with a role in the synthesis of cyanidin and peonidin pigments.

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Multiple Allelism in Flavonoid Hydroxylation in Eustoma grandiflorum (Raf.) Shinn. Flowers

Cited by 3 publications

References 34 publications

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Flavonoid 3',5'-Hydroxylase (<i>F3'5'H</i>) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [<i>Eustoma grandiflorum</i> (Raf.) Shinn.]

Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>

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Multiple Allelism in Flavonoid Hydroxylation in Eustoma grandiflorum (Raf.) Shinn. Flowers

Cited by 3 publications

References 34 publications

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

A 94-bp Deletion of Anthocyanidin Synthase Gene in Acyanic Flower Lines of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.]

Flavonoid 3',5'-Hydroxylase (<i>F3'5'H</i>) Gene Polymorphisms Co-segregate with Variation in Anthocyanin Composition in the Flower Petals of Lisianthus [<i>Eustoma grandiflorum</i> (Raf.) Shinn.]

Cloning of the Flavonoid 3&apos;-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3&apos;H</i>) and Complementation of an F3&apos;H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3&apos;H</i>

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Cloning of the Flavonoid 3'-Hydroxylase Gene of <i>Eustoma grandiflorum</i> (Raf.) Shinn. (<i>EgF3'H</i>) and Complementation of an F3'H-deficient Mutant of <i>Ipomoea nil</i> (L.) Roth. by Heterologous Expression of <i>EgF3'H</i>