Multiphoton microscopy for tumor regression grading after neoadjuvant treatment for colorectal carcinoma

Li, Lian-Huang; Chen, Zhifen; Wang, Xingfu; Zhuo, Shuangmu; Li, Hongsheng; Jiang, Weizhong; Guan, Guoxian; Chen, Jianxin

doi:10.3748/wjg.v21.i14.4210

Cited by 12 publications

(6 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nonlinear optical microscopy has been extensively used for the diagnosis of cancer diseases based on specific estimating parameters, which are tabulated in Table 2 as an overview on the existing applications. Among them, the intensity ratio of TPEF/SHG has been used to differentiate between cancerous and normal tissues; 15,17,27,28 however, to our knowledge, this approach has not previously been used for the staging of ESCC. Here we investigated the single collagen fibers in submucosa of cancerous tissues and counted the intensities of TPEF and SHG for ratiometric analysis.…”

Section: Resultsmentioning

confidence: 99%

Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy

Chen

Lin

et al. 2020

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

A quantitative analytical method to discriminate among the various types of cancerous esophagus tissue is essential for accurate cancer staging. This paper reports on the use of ratiometric nonlinear optical microscopy to reveal the ratio of two-photon excited fluorescence (TPEF) to second harmonic generation (SHG) and forward to backward (F/B) SHG from single collagen fibers only in submucosa of esophageal squamous cell carcinoma. This makes it possible to accurately differentiate among the four stages of esophageal cancer, providing results that are in good agreement with histopathology. Furthermore, it is confirmed by polarization-dependent SHG that the varied SHG response in esophageal cancer tissues is mainly from the shrinkage in diameter of collagen fibers instead of the collagen triple helixes altered by cancer cells. Based on the results of TPEF/SHG and F/B SHG ratio, they can cooperatively improve the precision of diagnostics on esophageal cancer and could be transferred to other types of cancer diseases with changed collagen fibers. Impact statement The issue of classifying esophageal cancer at various developmental stages is crucial for determining the optimized treatment protocol for the patients, as well as the prognosis. Precision improvement in staging esophageal cancer keeps seeking quantitative and analytical imaging methods that could augment histopathological techniques. In this work, we used nonlinear optical microscopy for ratiometric analysis on the intrinsic signal of two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) from single collagen fibers only in submucosa of esophageal squamous cell carcinoma (ESCC). The blind tests of TPEF/SHG and forward (F)/backward (B) SHG were demonstrated to compare with the histology conclusion. The discussion of sensitivity and specificity was provided via statistical comparison between the four stages of esophageal cancer. To the best of our knowledge, this is the first study of using these two ratios in combination for staging ESCC.

show abstract

Section: Resultsmentioning

confidence: 99%

Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy

Chen

Lin

et al. 2020

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

show abstract

“…Dynamic contrast enhanced MRI and positron emission tomography may be valuable to evaluate the response of CRT . A recent study showed that multiphoton microscopy could be used to perform real‐time diagnosis of tumour response for colorectal cancer after neoadjuvant treatment . Microarray gene expression analysis suggested a gene set comprising the top‐ranked 95 genes to predict complete response to CRT .…”

Section: Discussionmentioning

confidence: 99%

Selecting stage ypT0–1N0 for locally advanced rectal cancer following preoperative chemoradiotherapy: implications for potential candidates of organ‐sparing management

Huang

Chi

et al. 2016

Colorectal Disease

View full text Add to dashboard Cite

show abstract

“…The radiation-induced changes of normal rectum tissue such as degradation and subsequent remodeling of collagen also can be estimated by using SHG imaging [31]. The extent of stromal fibrosis and observation of residual tumors after neoadjuvant radiochemotherapy can be established to distinguish tumor regression grading of colorectal adenocarcinomas treated with neoadjuvant therapy (figure 3) [32].…”

Section: Evaluating Colorectal Cancer Therapy Responsementioning

confidence: 99%

“…Scale bar: 100 µm. Adapted from [32]. multiphoton absorption has also been used as a useful tool for the selective ablation of tissue structures.…”

Section: Visualizing and Ablating Pre-invasive Colorectal Cancer Cellsmentioning

confidence: 99%

Applications of multiphoton microscopy in the field of colorectal cancer

Wang¹,

Li²,

Zhu³

et al. 2018

Laser Phys.

View full text Add to dashboard Cite

Multiphoton microscopy (MPM) is a powerful tool for visualizing cellular and subcellular details within living tissue by its unique advantages of being label-free, its intrinsic optical sectioning ability, near-infrared excitation for deep penetration depth into tissue, reduced photobleaching and phototoxicity in the out-of-focus regions, and being capable of providing quantitative information. In this review, we focus on applications of MPM in the field of colorectal cancer, including monitoring cancer progression, detecting tumor metastasis and microenvironment, evaluating the cancer therapy response, and visualizing and ablating preinvasive cancer cells. We also present one of the major challenges and the future research direction to exploit a colorectal multiphoton endoscope.

show abstract

Multiphoton microscopy for tumor regression grading after neoadjuvant treatment for colorectal carcinoma

Abstract: MPM can be used to perform real-time diagnosis of tumor response after neoadjuvant treatment, and can be applied to evaluate TRG.

Cited by 12 publications

References 30 publications

Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy

Label-free characterization of collagen fibers in cancerous esophagus tissues using ratiometric nonlinear optical microscopy

Selecting stage ypT0–1N0 for locally advanced rectal cancer following preoperative chemoradiotherapy: implications for potential candidates of organ‐sparing management

Applications of multiphoton microscopy in the field of colorectal cancer

Contact Info

Product

Resources

About