Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

Chen, Youting; Chen, Jing; Chen, Hong; Hong, Zhipeng; Zhu, Xiaodong; Zhuo, Shuangmu; Chen, Yanling; Chen, Jianxin

doi:10.1117/1.jbo.19.9.096008

Cited by 11 publications

(7 citation statements)

References 28 publications

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“…20 cells with cytoplasm and nuclei demonstrating complete and clearly discernible outlines were selected in each TPEF or H&E stained image. And then the cells were circled and measured by using an optional fine‐focusing stage (HRZ 200, Carl Zeiss) (Chen et al ., ).…”

Section: Methodsmentioning

confidence: 97%

Two-photon excited fluorescence imaging of the pancreatic solid pseudopapillary tumor without hematoxylin and eosin stains

Liao

Chen

et al. 2015

Scanning

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Solid pseudopapillary tumor (SPT) of the pancreas is an epithelial tumor with low-grade malignant potential and present more common in females. At present, the gold standard for accurate diagnosis of pancreatic tumor was mostly depending on the pathological and/or cytological evaluation. In this work, TPEF microscopy was applied to obtain the images of human normal pancreas and SPT of the pancreas without hematoxylin and eosin (H&E) staining, for the purpose of identifying the organization structural, cell morphological, and cytoplasm changing, which were then compared to their corresponding H&E stained histopathological results. Our results showed that high-resolution TPEF imaging of the pancreatic SPT can clearly distinguish the pathological features from normal pancreas in unstained histological sections, and the results are consistent with the histological results. Moreover, we measured the nuclear-cytoplasmic ratios of the pancreatic SPT and normal pancreas to characterize their difference in the cytomorphological feature. It indicated that this technique can achieve the consistent information of pathological diagnosis, and has the potential to substantially improve the optical diagnosis and treatment of the pancreatic SPT without H&E staining in the future. SCANNING 38:245-250, 2016. © 2015 Wiley Periodicals, Inc.

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Section: Methodsmentioning

confidence: 97%

Two-photon excited fluorescence imaging of the pancreatic solid pseudopapillary tumor without hematoxylin and eosin stains

Liao

Chen

et al. 2015

Scanning

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“…Another group has demonstrated that MPM images clearly distinguish between structures of normal versus tumorigenic pancreatic tissues [87]. Figure 3 presents images of normal and deteriored pancreatic tissues imaged by MPM and by standard H&E staining, extracted from [87] with author's and editor's reprint permissions. Thanks to the colocalization, it appears that unstained multiphoton images deliver similar information with a better resolution than H&E staining.…”

Section: Striking Application Of Biomedical Mpmmentioning

confidence: 99%

A review of biomedical multiphoton microscopy and its laser sources

Lefort¹

2017

J. Phys. D: Appl. Phys.

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Multiphoton microscopy (MPM) has been the subject of major development efforts for about 25 years for imaging biological specimens at micron scale and presented as an elegant alternative to classical fluorescence methods such as confocal microscopy. In this topical review, the main interests and technical requirements of MPM are addressed with a focus on the crucial role of excitation source for optimization of multiphoton processes. Then, an overview of the different sources successfully demonstrated in literature for MPM is presented, and their physical parameters are inventoried. A classification of these sources in function with their ability to optimize multiphoton processes is proposed, following a protocol found in literature. Starting from these considerations, a suggestion of a possible identikit of the ideal laser source for MPM concludes this topical review.

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“…Mucins and proteoglycans and is routinely used in pathology (as described in 50 ). The cytoplasmic hematoxylin staining in the ducts cells in Fig.…”

Section: Concentrated Hematoxylin At Normal Ph Binds Tomentioning

confidence: 99%

Noninvasivein vivoimaging of embryonic β-cell development in the anterior chamber of the eye

Cras-Méneur

Elghazi

Fort

et al. 2016

Islets

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The fetal environment plays a decisive role in modifying the risk for developing diabetes later in life. Developing novel methodology for noninvasive imaging of β-cell development in vivo under the controlled physiological conditions of the host can serve to understand how this environment affects β-cell growth and differentiation. A number of culture models have been designed for pancreatic rudiment but none match the complexity of the in utero or even normal physiological environment. Speier et al. recently developed a platform of noninvasive in vivo imaging of pancreatic islets using the anterior chamber of the eye where islets get vascularized, grow and respond to physiological changes. The same methodology was adapted for the study of pancreatic development. E13.0, still undifferentiated rudiments with fluorescent lineage tracing were implanted in the AC of the eye, allowing the longitudinal study of their growth and differentiation. Within 48 h the anlages get vascularized and grow but their mesenchyme displays a selective growth advantage. The resulting imbalance leads to alteration in the differentiation pattern of the progenitors. Reducing the mesenchyme to its bare minimum before implantation allows the restoration of a proper balance and a development that mimics the normal pancreatic development. These groundbreaking observations demonstrate that the anterior chamber of the eye provides a good system for noninvasive in vivo fluorescence imaging of the developing pancreas under the physiology of the host and can have important implications for designing strategies to prevent or reverse the deleterious effects of hyperglycemia on altering β-cell function later in life.

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Multiphoton microscopy as a diagnostic imaging modality for pancreatic neoplasms without hematoxylin and eosin stains

Cited by 11 publications

References 28 publications

Two-photon excited fluorescence imaging of the pancreatic solid pseudopapillary tumor without hematoxylin and eosin stains

Two-photon excited fluorescence imaging of the pancreatic solid pseudopapillary tumor without hematoxylin and eosin stains

A review of biomedical multiphoton microscopy and its laser sources

Noninvasivein vivoimaging of embryonic β-cell development in the anterior chamber of the eye

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